Human/Mouse IL-1ra/IL-1F3 Antibody

Name: Human/Mouse IL-1ra/IL-1F3 Antibody
Brand: R&D Systems
SKU: MAB480
Rating: 4.5 (2 reviews)

Catalog #: MAB480
2 Citations 2 Reviews Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB480
MAB480-SP

All IL-1ra/IL-1F3 Antibodies

Product Details

Citations (2)

FAQs

Supplemental Products

Reviews (2)

Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse IL-1ra/IL-1F3 Antibody Summary

Species Reactivity

Human, Mouse

Specificity

Detects human and mouse IL-1ra in direct ELISAs and Western blots.

Source

Monoclonal Rat IgG_2A Clone # 40007

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant mouse IL-1ra
Arg27-Gln178
Accession # P25085

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Human IL-1ra/IL-1F3 (Catalog # 280-RA)
Recombinant Mouse IL-1ra/IL-1F3 (Catalog # 480-RM)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-1ra/IL-1F3

IL-1ra (IL-1 receptor antagonist) is a widely expressed acute phase protein that serves to dampen inflammation. IL-1ra binds to both IL-1 RI and IL-1 RII. It competitively inhibits IL-1 (alpha or beta) binding to IL-1 RI and does not trigger recruitment of the accessory molecule IL-1 R AcP or activation of IL-1 RI signaling. IL-1ra blocks the proinflammatory actions of IL-1 including Prostaglandin E2 and IL-6 release from endothelial cells, fever generation, thrombocytosis, the production of hepatic acute phase proteins, and neutrophil expansion and infiltration.

Long Name

Interleukin 1 Receptor Antagonist

Entrez Gene IDs

3557 (Human); 16181 (Mouse); 60582 (Rat); 397499 (Porcine)

Alternate Names

DIRA; ICIL-1ra; IL1F3; IL-1F3; IL1ra; IL-1ra; IL-1ra3; IL1RN; IL-1RN; interleukin 1 receptor antagonist; IRAP; MVCD4

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human/Mouse IL-1ra/IL-1F3 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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Characterization of the TNF and IL-1 systems in human brain and blood after ischemic stroke
Authors: BH Clausen, M Wirenfeldt, SS Høgedal, LH Frich, HH Nielsen, HD Schrøder, K Østergaard, B Finsen, BW Kristensen, KL Lambertsen
Acta Neuropathol Commun, 2020-06-05;8(1):81.
Species: Human
Sample Types: Whole Tissue
Applications: IHC
Transplantation of skeletal myoblasts secreting an IL-1 inhibitor modulates adverse remodeling in infarcted murine myocardium.
Authors: Murtuza B, Suzuki K, Bou-Gharios G, Beauchamp JR, Smolenski RT, Partridge TA, Yacoub MH
Proc. Natl. Acad. Sci. U.S.A., 2004-03-12;101(12):4216-21.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-P