Human/Mouse Jagged 1/Jagged 2 APC-conjugated Antibody Summary
Met27-Asp307
Accession # Q9Y219
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
View Larger
Detection of Jagged 1/Jagged 2 in HUVEC Human Cells by Flow Cytometry. HUVEC human umbilical vein endothelial cells were stained with Mouse Anti-Human/Mouse Jagged 1/Jagged 2 APC-conjugated Monoclonal Antibody (Catalog # FAB1726A, filled histogram) or isotype control antibody (Catalog # IC0041A, open histogram). View our protocol for Staining Membrane-associated Proteins.
View Larger
Detection of Jagged 1/Jagged 2 in D3 Mouse Cell Line by Flow Cytometry. D3 mouse embryonic stem cell line was stained with Mouse Anti-Human/Mouse Jagged 1/Jagged 2 APC-conjugated Monoclonal Antibody (Catalog # FAB1726A, filled histogram) or isotype control antibody (Catalog # IC0041A, open histogram). View our protocol for Staining Membrane-associated Proteins.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: Jagged 1/Jagged 2
Human Jagged 2 is a 131 kDa (predicted) member of the Delta-Serrate-Lag-2 (DSL) family of ligands. This family activates LIN12/Notch proteins and thereby regulates cell fate determination during development (1-5). It is a type 1 transmembrane protein that is synthesized as a 1238 amino acid (aa) precursor. It contains a 23 aa signal sequence, a large 1057 aa extracellular region, a 21 aa transmembrane region, and a short 137 aa cytoplasmic region. The extracellular region contains four potential N-linked glycosylation sites, a DSL domain, 16 EGF-like repeats (many of which are also sites of calcium binding), a von Willebrand factor (vWF) type C domain, and a cysteine-rich region just proximal to the transmembrane segment (2). There are two isoforms for human Jagged 2, named long and short. The short form lacks a splicing variant region (aa 421-461) that is present in the long form of the protein. Human Jagged 2 shares 90% and 87% aa sequence identity with mouse and rat Jagged 2, respectively. During murine embryonic development, Jagged 2 is expressed highest in fetal thymus, epidermis, foregut, dorsal root ganglia, and inner ear (2). In 2 week old mice, the Jagged 2 transcript is most abundant in heart, lung, thymus, skeletal muscle, brain, and testis (2). Functionally, it is suggested that Jagged 2 engages the Notch1 pathway of signal transduction (2). It is involved in the development of the mammalian limb, branchial arches, central and peripheral nervous systems, T cell lineage differentiation, natural killer cells, and the establishment of functional natural killer cell lines (3, 5, 6).
- Shawber, C. et al. (1996) Dev. Biol. 180:370.
- Luo, B. et al. (1997) Mol. Cell. Biol. 17:6057.
- Valsecchi, V. et al. (1997) Mech. Dev. 69:203.
- Schickwann, T. et al. (2000) Blood 96:950.
- DeHart, S. et al. (2005) Blood 105:3521.
- de La Coste, A. and A.A. Freitas (2006) Immunol. Lett. 102:1.
Product Datasheets
Citations for Human/Mouse Jagged 1/Jagged 2 APC-conjugated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
2
Citations: Showing 1 - 2
Filter your results:
Filter by:
-
NOTCH-mediated non-cell autonomous regulation of chromatin structure during senescence
Authors: AJ Parry, M Hoare, D Bihary, R Hänsel-Her, S Smith, K Tomimatsu, E Mannion, A Smith, P D'Santos, IA Russell, S Balasubram, H Kimura, SA Samarajiwa, M Narita
Nat Commun, 2018-05-09;9(1):1840.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
NOTCH1 mediates a switch between two distinct secretomes during senescence
Authors: M Hoare, Y Ito, TW Kang, MP Weekes, NJ Matheson, DA Patten, S Shetty, AJ Parry, S Menon, R Salama, R Antrobus, K Tomimatsu, W Howat, PJ Lehner, L Zender, M Narita
Nat. Cell Biol, 2016-08-15;18(9):979-92.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
FAQs
No product specific FAQs exist for this product, however you may
View all Antibody FAQsReviews for Human/Mouse Jagged 1/Jagged 2 APC-conjugated Antibody
There are currently no reviews for this product. Be the first to review Human/Mouse Jagged 1/Jagged 2 APC-conjugated Antibody and earn rewards!
Have you used Human/Mouse Jagged 1/Jagged 2 APC-conjugated Antibody?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
