Human/Mouse Meprin beta Subunit/MEP1B Antibody

Catalog #: MAB28951
4 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB28951
MAB28951-SP

Detection of Human and Mouse Meprin beta Subunit/ MEP1B by Western Blot.

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Citations (4)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse Meprin beta Subunit/MEP1B Antibody Summary

Species Reactivity

Human, Mouse

Specificity

Detects the pro form of human or mouse Meprin beta Subunit/MEP1B in Western blots. It does not react with the mature form.

Source

Monoclonal Rat IgG₁ Clone # 289731

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Human embryonic kidney cell line HEK293-derived recombinant human Meprin beta Subunit/MEP1B
Thr23-Ser293
Accession # Q16820

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Immunohistochemistry

8-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human and Mouse Meprin beta Subunit/ MEP1B by Western Blot. Western blot shows lysates of human intestine tissue and mouse intestine tissue. PVDF membrane was probed with 1 µg/mL of Rat Anti-Human/Mouse Meprin beta Subunit/MEP1B Monoclonal Antibody (Catalog # MAB28951) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Meprin beta Subunit/MEP1B at approximately 97 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry

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Meprin beta Subunit/MEP1B in Human Intestine. Meprin beta Subunit/MEP1B was detected in immersion fixed paraffin-embedded sections of human intestine using Human/Mouse Meprin beta Subunit/MEP1B Monoclonal Antibody (Catalog # MAB28951) at 1 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hemotoxylin (blue). Specific staining was localized to the Brush border of epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections. This application has not been tested in mouse samples

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Meprin beta Subunit/MEP1B

Meprins are multimeric proteases composed of alpha and beta subunits, which are members of the astacin family of zinc endopeptidases (1, 2). Both subunits form disulfide-linked homo- or heterooligomers, which are also referred to as Meprin A (composed of alpha subunits with or without beta subunits) and Merpin B (composed of beta subunits only) (3). Although the two subunits share 42% amino acid (aa) sequence identity, they differ significantly in their oligomeric structure, post-translational processing and subsequently cellular location, and substrate and peptide bond specificity (4). The 701 aa sequence of human Merpin beta subunit precursor consists of a signal peptide (aa 1 to 21), a pro region (aa 22 to 61), and a mature chain (aa 62 to 701) containing catalytic (aa 62 to 259), MAM (aa 260 to 429), MATH (aa 430 to 585), EGF-like (aa 604 to 644), transmembrane (aa 653 to 673), and cytoplasmic (aa 674 to 701) domains.

References

Bond, J.S. and R.J. Beynon (1995) Protein Sci. 4:1247.
Stocker, W. et al. (1995) Protein Sci. 4:823.
Bertenshaw, G.P. et al. (2001) J. Biol. Chem. 276:13248.
Ishmael, F.T. et al. (2005) J. Biol. Chem. 280:13895.

Entrez Gene IDs

4225 (Human); 17288 (Mouse)

Alternate Names

EC 3.4.24.63; endopeptidase-2; MEP1B; meprin A subunit beta; meprin A, beta; Meprin B; Meprin beta Subunit; N-benzoyl-L-tyrosyl-p-amino-benzoic acid hydrolase beta subunit; N-benzoyl-L-tyrosyl-P-amino-benzoic acid hydrolase subunit beta; PABA peptide hydrolase; PPH beta

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Citations for Human/Mouse Meprin beta Subunit/MEP1B Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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