Human/Mouse Nbs1 Antibody

Catalog #: MAB15731 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB15731
MAB15731-SP

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Human/Mouse Nbs1 Antibody Summary

Species Reactivity

Human, Mouse

Specificity

Detects human and mouse Nbs1.

Source

Monoclonal Mouse IgG₁ Clone # 206911

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human Nbs1
Pro498-Arg754
Accession # O60934

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Purity

Protein A or G purified from hybridoma culture supernatant

Applications

Recommended Concentration

Sample

Immunoprecipitation

2 µg/500 µg cell lysate

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunoprecipitation

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Immunoprecipitation of Human Nbs1. Nbs1 was immunoprecipitated from 500 µg of K562 human chronic myelogenous leukemia cell line lysate following incubation with 2 µg Mouse Anti-Human/Mouse Nbs1 Mono-clonal Antibody (Catalog # MAB15731) for 1 hour on ice. Nbs1-antibody complexes were absorbed using goat anti-mouse IgG-agarose (Sigma). Immunoprecipitated Nbs1 was detected by Western blot using 1 µg/mL Human/Mouse/Rat Nbs1 Mono-clonal Antibody (Catalog # MAB1573). View our recommended buffer recipes for immunoprecipitation.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Nbs1

The Nijmegen Breakage Syndrome 1 (Nbs1) protein, also called Nibrin, is a member of the Mre11/Rad50/Nbs1 (MRN) protein complex that binds to DNA double-strand breaks in cells exposed to DNA damaging agents. In addition, the MRN complex colocalizes with replication forks during DNA replication. The MRN complex plays an important role in routine cell cycle progression and genotoxic stress responses by facilitating DNA repair. In fact, mutation of the nbs1 gene and resultant loss of Nbs1 protein expression in humans results in the chromosomal instability disease, Nijmegen Breakage Syndrome.

Long Name

Nijmegen Breakage Syndrome 1

Entrez Gene IDs

4683 (Human); 27354 (Mouse); 85482 (Rat)

Alternate Names

ATV; AT-V1; AT-V2; Cell cycle regulatory protein p95; FLJ10155; MGC87362; NBN; NBS; Nbs1; NBS1P95; Nibrin; Nijmegen breakage syndrome 1 (nibrin); Nijmegen breakage syndrome protein 1; p95 protein of the MRE11/RAD50 complex; p95