Human/Mouse NLRP3/NALP3 Antibody Summary
Met1-Arg153
Accession # Q8R4B8
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
Detection of Mouse NLRP3/NALP3 by Western Blot. Western blot shows lysates of J774A.1 mouse reticulum cell sarcoma macrophage cell line and RAW 264.7 mouse monocyte/macrophage cell line. PVDF membrane was probed with 2 µg/mL of Rat Anti-Human/Mouse NLRP3/NALP3 Monoclonal Antibody (Catalog # MAB7578) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for NLRP3/NALP3 at approximately 117 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Catalog # AF6789is recommended for use with human lysates.
NLRP3/NALP3 in RAW 264.7 Mouse Cell Line. NLRP3/NALP3 was detected in immersion fixed RAW 264.7 mouse monocyte/macrophage cell line using Rat Anti-Human/Mouse NLRP3/NALP3 Monoclonal Antibody (Catalog # MAB7578) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the Northern-Lights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
NLRP3/NALP3 in Human Tonsil Tissue. NLRP3/NALP3 was detected in immersion fixed paraffin-embedded sections of human tonsil tissue using Rat Anti-Human/Mouse NLRP3/NALP3 Monoclonal Antibody (Catalog # MAB7578) at 5 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Rat IgG VisUCyte™ HRP Polymer Antibody (brown; Catalog # VC005) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in lymphocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Detection of NLRP3/NALP3 in RAW 264.7 Mouse Cell Line by Flow Cytometry. RAW 264.7 mouse monocyte/macrophage cell line was stained with Rat Anti-Human/Mouse NLRP3/ NALP3 Monoclonal Antibody (Catalog # MAB7578, filled histo-gram) or isotype control antibody (Catalog # MAB006, open histo-gram), followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.
Detection of NLRP3/NALP3 in Human Monocytes by Flow Cytometry. Human peripheral blood monocytes was stained with Rat Anti-Human/Mouse NLRP3/NALP3 Monoclonal Antibody (Catalog # MAB7578, filled histogram) or isotype control antibody (Catalog # MAB006, open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: NLRP3/NALP3
NLRP3, also known as NALP3, CIAS1, PYPAF or Cryopyrin, is a cytosolic ~120 kDa member of the NLRP family of proteins expressed in leukocytes, especially neutrophils. As a component of the inflammasome, NLRP3 activates caspases 1 and 5. Defects in NLRP3 may cause FCAS1, CINCA, or Muckle-Wells autoinflammatory syndromes. Mouse NLRP3 contains an N-terminal Pyrin domain (aa 1-91) followed by a Nacht region (aa 216-532), and seven LRRs (aa 739-988). Within the sequence used as an immunogen, mouse NLRP3 shares 78% and 93% aa identity with human and rat NLRP3, respectively. Alternate splicing of mouse NLRP3 generates additional isoforms that lack either LRR2 and 3, LRR 6 and 7, or LRR4-9.
Product Datasheets
Citations for Human/Mouse NLRP3/NALP3 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 10
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Toxoplasma gondii profilin induces NLRP3 activation and IL-1beta production/secretion in THP-1�cells
Authors: H Pazoki, H Mirjalali, M Niyyati, SJ Seyed Taba, N Mosaffa, S Shahrokh, H Asadzadeh, A Kupz, MR Zali
Microbial pathogenesis, 2023-04-18;180(0):106120.
Species: Human
Sample Types: Protein
Applications: Western Blot -
IRAK-M suppresses the activation of microglial NLRP3 inflammasome and GSDMD-mediated pyroptosis through inhibiting IRAK1 phosphorylation during experimental autoimmune encephalomyelitis
Authors: Y Wang, S Pei, Z Liu, Y Ding, T Qian, H Wen, SW Hsu, Z Zhou, J Zhang, H Wang
Cell Death & Disease, 2023-02-10;14(2):103.
Species: Mouse
Sample Types: Tissue Homogenates, Whole Tissue
Applications: IHC, Western Blot -
Toxoplasma gondii profilin induces NLRP3 activation and IL-1beta production/secretion in THP-1�cells
Authors: H Pazoki, H Mirjalali, M Niyyati, SJ Seyed Taba, N Mosaffa, S Shahrokh, H Asadzadeh, A Kupz, MR Zali
Microbial pathogenesis, 2023;180(0):106120.
Species: Human
Sample Types: Protein
Applications: Western Blot -
Attenuation of hepatic fibrosis by p-Coumaric acid via modulation of NLRP3 inflammasome activation in C57BL/6 mice
Authors: TMT Truong, SH Seo, S Chung, I Kang
The Journal of nutritional biochemistry, 2022-11-15;0(0):109204.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot -
Blockage of KHSRP-NLRP3 by MCC950 Can Reverse the Effect of Manganese-Induced Neuroinflammation in N2a Cells and Rat Brain
Authors: S Singh, IA Shaikh, SS More, MH Mahnashi, HM Almohaimee, M El-Sherbin, MM Ghoneim, A Umar, HK Soni, H Agrawal, BA Mannasaheb, AA Khan, UM Muddapur, SMS Iqubal
International Journal of Molecular Sciences, 2022-10-30;23(21):.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
Modulation of MAPK- and PI3/AKT-Dependent Autophagy Signaling by Stavudine (D4T) in PBMC of Alzheimer's Disease Patients
Authors: F La Rosa, CP Zoia, C Bazzini, A Bolognini, M Saresella, E Conti, C Ferrarese, F Piancone, I Marventano, D Galimberti, C Fenoglio, E Scarpini, M Clerici
Cells, 2022-07-12;11(14):.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Soluble Endoglin Stimulates Inflammatory and Angiogenic Responses in Microglia That Are Associated with Endothelial Dysfunction
Authors: ES Park, S Kim, DC Yao, JPJ Savarraj, HA Choi, PR Chen, E Kim
International Journal of Molecular Sciences, 2022-01-22;23(3):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
TRPV1 channel mediates NLRP3 inflammasome-dependent neuroinflammation in microglia
Authors: Y Zhang, B Hou, P Liang, X Lu, Y Wu, X Zhang, Y Fan, Y Liu, T Chen, W Liu, B Peng, J Yin, S Han, X He
Cell Death & Disease, 2021-12-14;12(12):1159.
Species: Mouse
Sample Types: Cell Lysates, Whole Cells, Whole Tissue
Applications: ICC, IHC, Western Blot -
Leptin Induces Apoptotic and Pyroptotic Cell Death via NLRP3 Inflammasome Activation in Rat Hepatocytes
Authors: A Baral, PH Park
International Journal of Molecular Sciences, 2021-11-22;22(22):.
Species: Rat
Sample Types: Cell Lysates
Applications: Western Blots -
Farrerol Ameliorated Cisplatin-Induced Chronic Kidney Disease Through Mitophagy Induction via Nrf2/PINK1 Pathway
Authors: N Ma, Z Wei, J Hu, W Gu, X Ci
Frontiers in Pharmacology, 2021-11-11;12(0):768700.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot -
IL-1 reprogramming of adult neural stem cells limits neurocognitive recovery after viral encephalitis by maintaining a proinflammatory state
Authors: AL Soung, VA Davé, C Garber, ED Tycksen, LL Vollmer, RS Klein
Brain, Behavior, and Immunity, 2021-10-22;0(0):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Cyanidin-3-O-Glucoside improves the viability of human islet cells treated with amylin or Abeta1-42 in vitro
Authors: J Croden, JR Silva, W Huang, N Gupta, W Fu, K Matovinovi, M Black, X Li, K Chen, Y Wu, J Jhamandas, GR Rayat
PLoS ONE, 2021-10-06;16(10):e0258208.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
AIM2 inflammasome activation may mediate high mobility group box 1 release in murine allergic rhinitis
Authors: Y Wang, S Chen, PL Yang, JJ Chen, WJ Kong, YJ Wang
Brazilian journal of otorhinolaryngology, 2021-02-15;0(0):.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
Gene expression network analyses during infection with virulent and avirulent Trypanosoma cruzi�strains unveil a role for fibroblasts in neutrophil recruitment and activation
Authors: AER Oliveira, MCA Pereira, AT Belew, LRP Ferreira, LMN Pereira, EGA Neves, MDCP Nunes, BA Burleigh, WO Dutra, NM El-Sayed, RT Gazzinelli, SMR Teixeira
PLoS Pathog., 2020-08-18;16(8):e1008781.
Species: Human
Sample Types: Cell Lysates, Protein
Applications: Western Blot -
The Combination of Sulforaphane and Fernblock� XP Improves Individual Beneficial Effects in Normal and Neoplastic Human Skin Cell Lines
Authors: S Serini, R Guarino, R Ottes Vasc, L Celleno, G Calviello
Nutrients, 2020-05-30;12(6):.
Species: Human
Sample Types: Cell Culture Lysates
Applications: Western Blot -
Inhibition of the NLRP3-inflammasome prevents cognitive deficits in experimental autoimmune encephalomyelitis mice via the alteration of astrocyte phenotype
Authors: B Hou, Y Zhang, P Liang, Y He, B Peng, W Liu, S Han, J Yin, X He
Cell Death Dis, 2020-05-15;11(5):377.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot -
Targeting Macrophage Histone H3 Modification as a Leishmania Strategy to Dampen the NF-&kappaB/NLRP3-Mediated Inflammatory Response
Authors: H Lecoeur, E Prina, T Rosazza, K Kokou, P N'Diaye, N Aulner, H Varet, G Bussotti, Y Xing, G Milon, R Weil, G Meng, GF Späth
Cell Rep, 2020-02-11;30(6):1870-1882.e4.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
NLRP3 Inflammasome and Mineralocorticoid Receptors Are Associated with Vascular Dysfunction in Type 2 Diabetes Mellitus
Authors: NS Ferreira, T Bruder-Nas, CA Pereira, CZ Zanotto, DS Prado, JF Silva, DM Rassi, MC Foss-Freit, JC Alves-Filh, D Carlos, RC Tostes
Cells, 2019-12-08;8(12):.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot -
Magnesium Sulfate Mitigates the Progression of Monocrotaline Pulmonary Hypertension in Rats
Authors: CY Chang, HJ Shih, IT Huang, PS Tsai, KY Chen, CJ Huang
Int J Mol Sci, 2019-09-18;20(18):.
Species: Rat
Sample Types: Whole Tissue
Applications: IHC-P -
DDX3X acts as a live-or-die checkpoint in stressed cells by regulating NLRP3 inflammasome
Authors: P Samir, S Kesavardha, DM Patmore, S Gingras, RKS Malireddi, R Karki, CS Guy, B Briard, DE Place, A Bhattachar, BR Sharma, A Nourse, SV King, A Pitre, AR Burton, S Pelletier, RJ Gilbertson, TD Kanneganti
Nature, 2019-09-11;0(0):.
Species: Mouse
Sample Types: Whole Cells
Applications: ICC -
Anti-Irritant and Anti-Inflammatory Effects of DHA Encapsulated in Resveratrol-Based Solid Lipid Nanoparticles in Human Keratinocytes
Authors: S Serini, R Cassano, E Facchinett, G Amendola, S Trombino, G Calviello
Nutrients, 2019-06-21;11(6):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Evidence that TLR4 Is Not a Receptor for Saturated Fatty Acids but Mediates Lipid-Induced Inflammation by Reprogramming Macrophage Metabolism
Authors: GI Lancaster, KG Langley, NA Berglund, HL Kammoun, S Reibe, E Estevez, J Weir, NA Mellett, G Pernes, JRW Conway, MKS Lee, P Timpson, AJ Murphy, SL Masters, S Gerondakis, N Bartonicek, DC Kaczorowsk, ME Dinger, PJ Meikle, PJ Bond, MA Febbraio
Cell Metab., 2018-04-19;0(0):.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
Downregulation of the NLRP3 inflammasome by adiponectin rescues Duchenne muscular dystrophy
Authors: R Boursereau, M Abou-Samra, S Lecompte, L Noel, SM Brichard
BMC Biol., 2018-03-20;16(1):33.
Species: Mouse
Sample Types: Tissue Homogenates, Whole Tissue
Applications: IHC-P, Western Blot -
Eruca sativa seed extract: A novel natural product able to counteract neuroinflammation
Authors: A Gugliandol, S Giacoppo, M Ficicchia, A Aliquò, P Bramanti, E Mazzon
Mol Med Rep, 2018-03-07;0(0):.
Species: Mouse
Sample Types: Whole Cells
Applications: ICC -
Endothelial GPR124 Exaggerates the Pathogenesis of Atherosclerosis by Activating Inflammation
Authors: DM Gong, YL Zhang, DY Chen, LJ Hong, F Han, QB Liu, JJ Jiang, YM Lu
Cell. Physiol. Biochem., 2018-01-25;45(2):547-557.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Issues with the Specificity of Immunological Reagents for NLRP3: Implications for Age-related Macular Degeneration
Authors: C Kosmidou, NE Efstathiou, MV Hoang, S Notomi, EK Konstantin, M Hirano, K Takahashi, DE Maidana, P Tsoka, L Young, ES Gragoudas, TW Olsen, Y Morizane, JW Miller, DG Vavvas
Sci Rep, 2018-01-11;8(1):461.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
Expression of TLR2, NOD1, and NOD2 and the NLRP3 Inflammasome in Renal Tubular Epithelial Cells of Male versus Female Mice
Authors: SE DeWolf, AA Shigeoka, A Scheinok, SG Kasimsetty, AK Welch, DB McKay
Nephron, 2017-06-15;0(0):.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
Intestinal fungi contribute to development of alcoholic liver disease
Authors: AM Yang, T Inamine, K Hochrath, P Chen, L Wang, C Llorente, S Bluemel, P Hartmann, J Xu, Y Koyama, T Kisseleva, MG Torralba, K Moncera, K Beeri, CS Chen, K Freese, C Hellerbran, SM Lee, HM Hoffman, WZ Mehal, G Garcia-Tsa, EA Mutlu, A Keshavarzi, GD Brown, SB Ho, R Bataller, P Stärkel, DE Fouts, B Schnabl
J. Clin. Invest., 2017-05-22;0(0):.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
NLRP3 signaling drives macrophage-induced adaptive immune suppression in pancreatic carcinoma
Authors: D Daley, VR Mani, N Mohan, N Akkad, GSDB Pandian, S Savadkar, KB Lee, A Torres-Her, B Aykut, B Diskin, W Wang, MS Farooq, AI Mahmud, G Werba, EJ Morales, S Lall, BJ Wadowski, AG Rubin, ME Berman, R Narayanan, M Hundeyin, G Miller
J. Exp. Med., 2017-04-25;0(0):.
Species: Human, Mouse
Sample Types: Whole Cells, Whole Tissue
Applications: Flow Cytometry, IHC, IHC-P -
Inhibition of NLRP3 Inflammasome Pathway by Butyrate Improves Corneal Wound Healing in Corneal Alkali Burn
Authors: F Bian, Y Xiao, M Zaheer, EA Volpe, SC Pflugfelde, DQ Li, CS de Paiva
Int J Mol Sci, 2017-03-05;18(3):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-Fr, Western Blot -
The Antimalarial Chloroquine Suppresses LPS-Induced NLRP3 Inflammasome Activation and Confers Protection against Murine Endotoxic Shock
Authors: X Chen, N Wang, Y Zhu, Y Lu, X Liu, J Zheng
Mediators Inflamm., 2017-02-22;2017(0):6543237.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
Knockout of Ccr2 alleviates photoreceptor cell death in rodent retina exposed to chronic blue light
Cell Death Dis, 2016-11-10;7(11):e2468.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-Fr -
Suppression of NLRP3 inflammasome by gamma-tocotrienol ameliorates type 2 diabetes.
Authors: Kim Y, Wang W, Okla M, Kang I, Moreau R, Chung S
J Lipid Res, 2015-12-01;57(1):66-76.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
Chinese Herbal Preparation Xuebijing Potently Inhibits Inflammasome Activation in Hepatocytes and Ameliorates Mouse Liver Ischemia-Reperfusion Injury.
Authors: Liu X, Hu Z, Zhou B, Li X, Tao R
PLoS ONE, 2015-07-01;10(7):e0131436.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot -
Caspase-8 as an Effector and Regulator of NLRP3 Inflammasome Signaling.
Authors: Antonopoulos C, Russo H, El Sanadi C, Martin B, Li X, Kaiser W, Mocarski E, Dubyak G
J Biol Chem, 2015-06-22;290(33):20167-84.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
The cyclopentenone prostaglandin 15d-PGJ2 inhibits the NLRP1 and NLRP3 inflammasomes.
Authors: Maier N, Leppla S, Moayeri M
J Immunol, 2015-02-13;194(6):2776-85.
Species: Mouse
Sample Types: Cell Lysates
Applications: Immunoprecipitation -
ASC in renal collecting duct epithelial cells contributes to inflammation and injury after unilateral ureteral obstruction.
Authors: Komada T, Usui F, Shirasuna K, Kawashima A, Kimura H, Karasawa T, Nishimura S, Sagara J, Noda T, Taniguchi S, Muto S, Nagata D, Kusano E, Takahashi M
Am J Pathol, 2014-03-05;184(5):1287-98.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot
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