Home / Oct-3/4 / Human/Mouse Oct-3/4 APC-conjugated Antibody

Human/Mouse Oct-3/4 APC-conjugated Antibody

Catalog #: IC1759A
4 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
IC1759A-100
IC1759A-025
Detection of Oct‑3/4 in BG01V Human Cells by Flow Cytometry.
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Product Details
Citations (4)
FAQs
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse Oct-3/4 APC-conjugated Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Detects human Oct-3/4 in Western blots and detects mouse Oct-3/4 in flow cytometry.
Source
Monoclonal Rat IgG2B Clone # 240408
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human Oct‑3/4
Met1-Asn265 (Met262Leu)
Accession # Q01860
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration
Sample
Intracellular Staining by Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Intracellular Staining by Flow Cytometry Detection of Oct‑3/4 antibody in BG01V Human Cells antibody by Flow Cytometry. View Larger

Detection of Oct‑3/4 in BG01V Human Cells by Flow Cytometry. BG01V human embryonic stem cells were stained with Rat Anti-Human/Mouse Oct-3/4 APC-conjugated Monoclonal Antibody (Catalog # IC1759A, filled histogram) or isotype control antibody (Catalog # IC013A, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Intracellular Staining by Flow Cytometry Detection of Oct‑3/4 antibody in D3 Mouse Cell Line antibody by Flow Cytometry. View Larger

Detection of Oct‑3/4 in D3 Mouse Cell Line by Flow Cytometry. D3 mouse embryonic stem cell line treated with 5 µM Retinoic Acid for 3 days was stained with Rat Anti-Human/Mouse Oct-3/4 APC-conjugated Monoclonal Antibody (Catalog # IC1759A, filled histogram) or isotype control antibody (Catalog # IC013A, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: Oct-3/4

Oct-3/4, also termed Oct-3 or Oct-4, is a POU transcription factor that is expressed in totipotent embryonic stem and germ cells.(2, 3) Oct-3/4 is required to sustain stem cell self-renewal and pluripotency.(4) It is considered a master regulator of pluripotency that controls lineage commitment and is the most widely recognized marker of totipotent embryonic stem cells.

References
  1. Takeda, J. et al. (1992) Nucleic Acids Res. 20:4613.
  2. Scholer, H.R. et al. (1989) EMBO J. 8:2543.
  3. Rosner, M.H. et al. (1990) Nature 345:686.
  4. Niwa, H. et al. (2000) Nat. Genet. 24:372.
Long Name
Octamer-binding Protein 3/4
Entrez Gene IDs
5460 (Human); 18999 (Mouse)
Alternate Names
3-Oct; Oct-3/4; OCT3;Oct-3;OCT4;Oct-4;OCT4;Oct3/4;OTF3;OTF-3;OTF4;POU domain protein;POU domain, class 5, transcription factor 1;POU5F1; Oct4; Otf3g; Otf4; Pou5f1

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Citations for Human/Mouse Oct-3/4 APC-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. Engineered cartilage utilizing fetal cartilage-derived progenitor cells for cartilage repair
    Authors: DY Park, BH Min, SR Park, HJ Oh, MD Truong, M Kim, JY Choi, IS Park, BH Choi
    Sci Rep, 2020-03-31;10(1):5722.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Generation of human erythroblast-derived iPSC line using episomal reprogramming system
    Authors: E Varga, M Hansen, T Wüst, M von Linder, E van den Ak
    Stem Cell Res, 2017-10-12;25(0):30-33.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. An alternative pluripotent state confers interspecies chimaeric competency.
    Authors: Wu J, Okamura D, Li M, Suzuki K, Luo C, Ma L, He Y, Li Z, Benner C, Tamura I, Krause M, Nery J, Du T, Zhang Z, Hishida T, Takahashi Y, Aizawa E, Kim N, Lajara J, Guillen P, Campistol J, Esteban C, Ross P, Saghatelian A, Ren B, Ecker J, Izpisua Belmonte J
    Nature, 2015-05-06;521(7552):316-21.
    Species: Human, Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. Murine cytomegalovirus infection of neural stem cells alters neurogenesis in the developing brain.
    Authors: Mutnal MB, Cheeran MC, Hu S
    PLoS ONE, 2011-01-13;6(1):e16211.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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