Human/Mouse PDGF-AA Quantikine ELISA Kit

Name: Human/Mouse PDGF-AA Quantikine ELISA Kit
Brand: R&D Systems
SKU: DAA00B
Rating: 5 (1 reviews)

Catalog #: DAA00B
20 Citations 1 Review Datasheet / COA / SDS

Discontinued Product

DAA00B has been discontinued.

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Citations (20)

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Summary Precision Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Human/Mouse PDGF-AA Quantikine ELISA Kit Summary

Assay Length

4.5 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (50 uL), Serum (10 uL), Platelet-poor EDTA Plasma (50 uL), Platelet-poor Heparin Plasma (50 uL), Saliva (25 uL)

Sensitivity

6.29 pg/mL

Assay Range

15.6 - 1,000 pg/mL (Cell Culture Supernates, Serum, Platelet-poor EDTA Plasma, Platelet-poor Heparin Plasma, Saliva)

Specificity

Natural and recombinant human and mouse PDGF-AA. Recombinant rat PDGF-AA shares 95.8% amino acid sequence identity with recombinant human PDGF-AA.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Product Summary

The Quantikine Human/Mouse PDGF-AA Immunoassay is a 4.5 hour solid-phase ELISA designed to measure PDGF-AA in human and mouse cell culture supernates, serum, plasma, and human saliva. It contains E. coli-expressed recombinant human PDGF-AA and has been shown to accurately quantitate the recombinant factor. Results obtained using natural PDGF-AA showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural human and natural mouse PDGF-AA.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates, Serum, Platelet-poor EDTA Plasma, Platelet-poor Heparin Plasma, Saliva

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	24	24	24
Mean (pg/mL)	126	469	835	123	417	792
Standard Deviation	10.9	38.7	67.4	14.8	50.6	83
CV%	8.7	8.3	8.1	12	12.1	10.5

Recovery

The recovery of PDGF-AA spiked to three different levels throughout the range of the assay in various matrices was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Media (n=4)	97	80-107
Platelet-poor EDTA Plasma (n=4)	88	70-100
Platelet-poor Heparin Plasma (n=4)	93	83-108

Linearity

To assess the linearity of the assay, samples containing or spiked with high concentrations of PDGF-AA were diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay. Samples were tested with two reage

Scientific Data

N/A

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Human/Mouse PDGF-AA Standard Curve

Product Datasheets

Product Datasheet

COA

SDS

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store the unopened product at 2 - 8 degreesC. Do not use past expiration date.

Background: PDGF-AA

The platelet-derived growth factor (PDGF) family of disulfide-linked dimeric proteins consists of four homodimeric proteins, PDGF-AA, PDGF-BB, PDGF-CC and PDGF-DD, and one heterodimeric protein, PDGF-AB. Members of this protein family act mainly on connective tissue. The dimeric isoforms of PDGF are expressed by different cell types and at different times during embryonic development. All PDGF isoforms are synthesized as inactive precursors and are processed to active forms that contain a PDGF/VEGF cysteine knot domain. Only PDGF-C and PDGF-D, also named spinal cord-derived growth factors SCDGF and SCDGF-B, also contain an N-terminal CUB domain. Mature PDGF dimers bind and induce the homo- or hetero-dimerization of two receptor tyrosine kinases (PDGF R alpha and R beta).

Long Name:

Platelet-derived Growth Factor AA

Entrez Gene IDs:

5154 (Human); 25266 (Rat)

Alternate Names:

PDGF1; PDGF-A; PDGFAA; PDGF-AA

Related Research Areas

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Citations for Human/Mouse PDGF-AA Quantikine ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

20 Citations: Showing 1 - 10
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Longitudinal multi-omics analyses of the gut-liver axis reveals metabolic dysregulation in hepatitis C infection and cirrhosis
Authors: RO Ali, GM Quinn, R Umarova, JA Haddad, GY Zhang, EC Townsend, L Scheuing, KL Hill, M Gewirtz, S Rampertaap, SD Rosenzweig, AT Remaley, JM Han, V Periwal, H Cai, PJ Walter, C Koh, EB Levy, DE Kleiner, O Etzion, T Heller
Nature Microbiology, 2022-12-15;0(0):.
Species: Human
Sample Types: Serum
Design, characterization and experimental validation of a compact, flexible pulsed power architecture for ex vivo platelet activation
Authors: AL Garner, A Caiafa, Y Jiang, S Klopman, C Morton, AS Torres, AM Loveless, VB Neculaes
PLoS ONE, 2017-07-26;12(7):e0181214.
Species: Human
Sample Types: Plasma
The production method affects the efficacy of platelet derivatives to expand mesenchymal stromal cells in vitro
Authors: M Bernardi, F Agostini, K Chieregato, E Amati, C Durante, M Rassu, M Ruggeri, S Sella, E Lombardi, M Mazzucato, G Astori
J Transl Med, 2017-05-01;15(1):90.
Species: Human
Sample Types: Cell Lysates
Combining bevacizumab and chemoradiation in rectal cancer. Translational results of the AXEBeam trial.
Authors: Verstraete M, Debucquoy A, Dekervel J, Van Pelt J, Verslype C, Devos E, Chiritescu G, Dumon K, D'Hoore A, Gevaert O, Sagaert X, Van Cutsem E, Haustermans K
Br J Cancer, 2015-03-17;112(8):1314-25.
Species: Human
Sample Types: Whole Blood
The systemic influence of platelet-derived growth factors on bone marrow mesenchymal stem cells in fracture patients.
Authors: Tan, Hiang Bo, Giannoudis, Peter V, Boxall, Sally A, McGonagle, Dennis, Jones, Elena
BMC Med, 2015-01-13;13(0):6.
Species: Human
Sample Types: Serum
Platelet-derived growth factor alpha mediates the proliferation of peripheral T-cell lymphoma cells via an autocrine regulatory pathway.
Authors: Piccaluga P, Rossi M, Agostinelli C, Ricci F, Gazzola A, Righi S, Fuligni F, Laginestra M, Mancini M, Sapienza M, De Renzo A, Tazzari P, Gibellini D, Went P, Alviano F, Zinzani P, Bagnara G, Inghirami G, Tripodo C, Pileri S
Leukemia, 2014-01-31;28(8):1687-97.
Species: Human
Sample Types: Cell Culture Supernates
Functional and differential proteomic analyses to identify platelet derived factors affecting ex vivo expansion of mesenchymal stromal cells.
Authors: Kinzebach S, Dietz L, Kluter H, Thierse H, Bieback K
BMC Cell Biol, 2013-10-30;14(0):48.
Species: Human
Sample Types: Cell Lysates
Platelet rich plasma clot releasate preconditioning induced PI3K/AKT/NFkappaB signaling enhances survival and regenerative function of rat bone marrow mesenchymal stem cells in hostile microenvironments.
Authors: Peng Y, Huang S, Wu Y, Cheng B, Nie X, Liu H, Ma K, Zhou J, Gao D, Feng C, Yang S, Fu X
Stem Cells Dev, 2013-08-30;22(24):3236-51.
Species: Human, Rat
Sample Types: Cell Culture Supernates, Platelet Rich Plasma (PRP)
Expression and prognostic value of circulating angiogenic cytokines in pancreatic cancer.
Authors: Rahbari NN, Schmidt T, Falk CS, Hinz U, Herber M, Bork U, Buchler MW, Weitz J, Koch M
BMC Cancer, 2011-07-05;11(0):286.
Species: Human
Sample Types: Serum
Leptin administered in physiological or pharmacological doses does not regulate circulating angiogenesis factors in humans.
Authors: Aronis KN, Diakopoulos KN, Fiorenza CG
Diabetologia, 2011-06-10;54(9):2358-67.
Species: Human
Sample Types: Serum

Deficiency of liver sinusoidal scavenger receptors stabilin-1 and -2 in mice causes glomerulofibrotic nephropathy via impaired hepatic clearance of noxious blood factors.
Authors: Schledzewski K, Geraud C, Arnold B, Wang S, Grone HJ, Kempf T, Wollert KC, Straub BK, Schirmacher P, Demory A, Schonhaber H, Gratchev A, Dietz L, Thierse HJ, Kzhyshkowska J, Goerdt S
J. Clin. Invest., 2011-02-01;121(2):703-14.
Species: Mouse
Sample Types: Plasma

Mesenchymal stem cells produce Wnt isoforms and TGF-beta1 that mediate proliferation and procollagen expression by lung fibroblasts.
Authors: Salazar KD, Lankford SM, Brody AR
Am. J. Physiol. Lung Cell Mol. Physiol., 2009-09-04;297(5):L1002-11.
Species: Mouse
Sample Types: Cell Culture Supernates

Dynamic change in phosphorylated platelet-derived growth factor receptor in peripheral blood leukocytes following docetaxel therapy predicts progression-free and overall survival in prostate cancer.
Authors: Mathew P, Thall PF, Wen S, Bucana C, Jones D, Horne E, Oh WK, Morris MJ, Lee YC, Logothetis CJ, Lin SH, Fidler IJ
Br. J. Cancer, 2008-10-07;99(9):1426-32.
Species: Human
Sample Types: Plasma

Pharmacodynamics and Pharmacokinetics of the Novel Thrombopoietin Mimetic Peptide RWJ-800088 in Humans.
Authors: Liem-Moolenaar M, Cerneus D, Molloy C, End D, Brown K, de Kam M, Cohen A, van Hensbergen Y, Burggraaf J
2008-10-01;84(0):481.
Species: Human
Sample Types: Plasma

Inhaled multiwalled carbon nanotubes potentiate airway fibrosis in murine allergic asthma.
Authors: Ryman-Rasmussen JP, Tewksbury EW, Moss OR, Cesta MF, Wong BA, Bonner JC
Am. J. Respir. Cell Mol. Biol., 2008-09-11;40(3):349-58.
Species: Mouse
Sample Types: BALF

Observations on the microvasculature of bone defects filled with biodegradable nanoparticulate hydroxyapatite.
Authors: Kilian O, Wenisch S, Karnati S, Baumgart-Vogt E, Hild A, Fuhrmann R, Jonuleit T, Dingeldein E, Schnettler R, Franke RP
Biomaterials, 2008-05-23;29(24):3429-37.
Species: Porcine
Sample Types: Plasma

Human bone marrow activates the Akt pathway in metastatic prostate cells through transactivation of the alpha-platelet-derived growth factor receptor.
Authors: Dolloff NG, Russell MR, Loizos N, Fatatis A
Cancer Res., 2007-01-15;67(2):555-62.
Species: Human
Sample Types: Cell Culture Supernates

Tumor-driven paracrine platelet-derived growth factor receptor alpha signaling is a key determinant of stromal cell recruitment in a model of human lung carcinoma.
Authors: Tejada ML, Yu L, Dong J, Jung K, Meng G, Peale FV, Frantz GD, Hall L, Liang X, Gerber HP, Ferrara N
Clin. Cancer Res., 2006-05-01;12(9):2676-88.
Species: Human
Sample Types: Cell Culture Supernates

Platelet-derived growth factor-AA is an essential and autocrine regulator of vascular endothelial growth factor expression in non-small cell lung carcinomas.
Authors: Shikada Y, Yonemitsu Y, Koga T, Onimaru M, Nakano T, Okano S, Sata S, Nakagawa K, Yoshino I, Maehara Y, Sueishi K
Cancer Res., 2005-08-15;65(16):7241-8.
Species: Human
Sample Types: Cell Culture Supernates

Expression of PDGF and their receptors in human retinal pigment epithelial cells and fibroblasts: regulation by TGF-beta.
Authors: Nagineni CN, Kutty V, Detrick B, Hooks JJ
J. Cell. Physiol., 2005-04-01;203(1):35-43.
Species: Human
Sample Types: Cell Culture Supernates