Human/Mouse PDZK1 Antibody

Catalog #: AF4997 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF4997
AF4997-SP

Detection of Human PDZK1 by Western Blot.

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Human/Mouse PDZK1 Antibody Summary

Species Reactivity

Human, Mouse

Specificity

Detects human and mouse PDZK1.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human PDZK1
Met1-Met519
Accession # Q5T2W1

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Immunohistochemistry

5-15 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human PDZK1 by Western Blot. Western blot shows lysates of Calu-6 human lung carcinoma cell line, U266 human myeloma cell line, MCF-7 human breast cancer cell line, and mouse liver tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human/Mouse PDZK1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4997) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for PDZK1 at approximately 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry

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PDZK1 in Human Kidney. PDZK1 was detected in immersion fixed paraffin-embedded sections of human kidney using Sheep Anti-Human/Mouse PDZK1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4997) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific labeling was localized to the brush border of proximal tubules. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PDZK1

PDZK1 (PDZ Domain-containing 1; also CFTR-associated protein of 70 kDa/CAP70) is a 65 - 70 kDa member of the NHER (Na/H Exchange Regulator) family of proteins. It is only expressed in epithelium, particularly that found in intestine, kidney and liver. PDZK1 serves as a scaffold protein that connects membrane proteins to cytoplasmic regulators. Human PDZK1 is 519 amino acids (aa) in length. It contains four PDZ domains (aa 9 - 90; 134 - 215; 243 - 323; and 378 - 458) plus two phosphorylation sites at Ser492 and Ser514. There are two potential truncated forms of human PDZK1, one that ends after Leu330, and a second that ends after Lys264. Human PDZK1 shares 78% aa identity with mouse PDZK1.

Long Name

PDZ Domain Containing 1

Entrez Gene IDs

5174 (Human); 59020 (Mouse); 65144 (Rat)

Alternate Names

CAP70; CFTR-associated protein of 70 kDa; CLAMP; Na(+)/H(+) exchanger regulatory factor 3; Na/Pi cotransporter C-terminal-associated protein 1; NaPi-Cap1; NHERF3; NHERF-3; NHERF3PDZD1Na(+)/H(+) exchange regulatory cofactor NHE-RF3; PDZ domain containing 1; PDZ domain-containing protein 1; PDZ-containing kidney protein 1; PDZD1; PDZK1; Sodium-hydrogen exchanger regulatory factor 3