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Human/Mouse Peroxiredoxin 3 Antibody

Catalog #: AF6610
2 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF6610
AF6610-SP
Detection of Human and Mouse Peroxiredoxin 3 by Western Blot.
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Citations (2)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse Peroxiredoxin 3 Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Detects human and mouse Peroxiredoxin 3 in direct ELISAs and Western blots. In direct ELISAs, approximately 6% cross-reactivity with recombinant mouse (rm) Peroxiredoxin 2 is observed and less than 1% cross-reactivity with recombinant human (rh) Peroxiredoxin 1, rmPeroxiredoxin 5, and rmPeroxiredoxin 6 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant mouse Peroxiredoxin 3
Met1-Gln257
Accession # P20108
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Purity
Immunogen affinity purified

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human and Mouse Peroxiredoxin 3 antibody by Western Blot. View Larger

Detection of Human and Mouse Peroxiredoxin 3 by Western Blot. Western blot shows lysates of TF-1 human erythroleukemic cell line and mouse spleen tissue. PVDF Membrane was probed with 1 µg/mL of Human/Mouse Peroxiredoxin 3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6610) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Peroxiredoxin 3 at approximately 28 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Peroxiredoxin 3

Peroxiredoxin-3 (Prx-3/III; also AOP-1, MER5 and thioredoxin-dependent peroxidase reductase) is a ubiquitous, 22-28 kDa mitochondrial antioxidant enzyme that belongs to the 2-Cys class of the TSA/ahpC family of peroxiredoxins. Prx-3 is known to act as either a homodimer, or a decamer, and scavenge reactive oxygen species generated by oxidative stress. The mouse Prx-3 precursor molecule is 257 amino acids (aa) in length. It contains a cleavable N-terminal 63 aa mitochondrial targeting sequence, plus a 194 aa mature enzyme that shows a thioredoxin domain between aa 64-222. There are two catalytic cysteines, one at Cys109 and another at Cys230 of the precursor. Prx-3 undergoes a phosphorylation at Thr147 that reduces its activity. One potential splice form shows a deletion of Arg149, Lys150 and Arg185. Full-length mouse Prx-3 is 86% and 95% aa identical to human and rat Prx-3, respectively.

Entrez Gene IDs
10935 (Human); 11757 (Mouse); 64371 (Rat)
Alternate Names
Antioxidant protein 1MGC104387; AOP-1; AOP-1MGC24293; AOP1PRO1748; EC 1.11.1.15; HBC189; MER5; Peroxiredoxin 3; Peroxiredoxin III; peroxiredoxin-3; PRDX3; Protein MER5 homolog; prx-III; SP-22; thioredoxin-dependent peroxide reductase, mitochondrial

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Citations for Human/Mouse Peroxiredoxin 3 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Electrophiles modulate glutathione reductase activity via alkylation and upregulation of glutathione biosynthesis
    Authors: S Jobbagy, DA Vitturi, SR Salvatore, L Turell, MF Pires, E Kansanen, C Batthyany, JR Lancaster, BA Freeman, FJ Schopfer
    Redox Biol, 2018-11-22;21(0):101050.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. NOX2 amplifies acetaldehyde-mediated cardiomyocyte mitochondrial dysfunction in alcoholic cardiomyopathy
    Sci Rep, 2016-09-14;6(0):32554.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot

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