Human/Mouse PKC alpha Antibody

Catalog #: MAB5340 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB5340
MAB5340-SP

Detection of Human/Mouse PKC alpha by Western Blot.

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Human/Mouse PKC alpha Antibody Summary

Species Reactivity

Human, Mouse

Specificity

Detects endogenous human and mouse PKC alpha in Western blots.

Source

Monoclonal Rat IgG_2B Clone # 421810

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human PKC alpha
Lys604-Val672
Accession # P17252

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human/Mouse PKC alpha by Western Blot. Western blot shows lysates of 293T human embryonic kidney cell line, Saos-2 human osteosarcoma cell line, and Balb/3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse PKCa Monoclonal Antibody (Catalog # MAB5340) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for PKCa at approximately 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PKC alpha

PKC alpha (Protein kinase C alpha) is an 80 kDa member of the PKC subfamily, AGC Ser/Thr protein kinase family of enzymes. It is widely expressed, and serves multiple cell-specific functions. PKC alpha is activated by increased diacylglycerol and intracellular calcium, and translocates to several sites such as the Golgi, nucleus and plasma membrane. Human PKC alpha contains two zinc finger regions (aa 36‑151), a protein kinase domain (aa 339‑557), and an AGC kinase region (aa 598‑668). Phosphorylations on Thr497, Thr638 and Ser657 are necessary for kinase activity. Over aa 604‑672, human PKC alpha shows 100% aa identity to mouse PKC alpha.

Long Name

Protein Kinase C alpha

Entrez Gene IDs

5578 (Human); 18750 (Mouse); 24680 (Rat); 447031 (Xenopus)

Alternate Names

AAG6; aging-associated gene 6; EC 2.7.11; EC 2.7.11.13; MGC129901; PKC alpha; PKCA; PKC-A; PKC-alpha; PKCAMGC129900; PRKACA; PRKCA; protein kinase C alpha type; protein kinase C, alpha