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Human/Mouse PRMT1 Antibody

Catalog #: AF6016
1 Citation Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF6016
AF6016-SP
Detection of Human and Mouse PRMT1 by Western Blot.
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Citations (1)
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Human/Mouse PRMT1 Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Detects human and mouse PRMT1 in Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human PRMT1
Lys218-Arg361
Accession # Q99873
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Purity
Immunogen affinity purified

Applications

Recommended Concentration
Sample
Western Blot
0.5 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human and Mouse PRMT1 antibody by Western Blot. View Larger

Detection of Human and Mouse PRMT1 by Western Blot. Western blot shows lysates of 293T human embryonic kidney cell line and Hepa 1-6 mouse hepatoma cell line. PVDF Membrane was probed with 0.5 µg/mL of Goat Anti-Human PRMT1 Polyclonal Antibody (Catalog # AF6016) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for PRMT1 at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PRMT1

PRMT/PRMT1 (Protein-Arginine Methyltransferase 1; also HRMT1L2 and HMT2) is a 42‑45 kDa type I member of the arginine N‑methyltransferase family of enzymes. It is ubiquitously expressed and dimethylates single arginine residue nitrogen atoms in a distributive manner (i.e.-with substrate release following each methlyation step). PRMT1 forms homodimers, and heterodimers with PRMT3, and exists as part of a 300‑400 kDa complex in vivo. Human PRMT1 is 361 amino acids in length and contains one adenosyl-L-methionine binding site (aa 85‑153) and a phosphorylation site at Tyr299. There are multiple splice variants that show distinct substrate specificities. There is an alternate start site 10 aa upstream of the standard start site, and a single Met, plus a five aa and 11 aa substiuition for aa 1‑19. One isoform has a 2 aa substitution for aa 1‑20 accompanied by a deketion of aa 52‑67. Over aa 218‑361, human PRMT1 is 100% identical in aa sequence to mouse PRMT1.

Long Name
Protein Arginine Methyltransferase 1
Entrez Gene IDs
3276 (Human)
Alternate Names
ANM1; EC 2.1.1; EC 2.1.1.-; EC 2.1.1.125; EC 2.1.1.77; HCP1; Histone-arginine N-methyltransferase PRMT1; HMT1 (hnRNP methyltransferase, S. cerevisiae)-like 2; HMT1 hnRNP methyltransferase-like 2 (S. cerevisiae); HMT1; HMT2; HRMT1L2; HRMT1L2HMT1 hnRNP methyltransferase-like 2; Interferon receptor 1-bound protein 4; IR1B4; IR1B4protein arginine N-methyltransferase 1; PRMT1; protein arginine methyltransferase 1

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Citation for Human/Mouse PRMT1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. L-Arginine/Nitric Oxide Pathway Is Altered in Colorectal Cancer and Can Be Modulated by Novel Derivatives from Oxicam Class of Non-Steroidal Anti-Inflammatory Drugs
    Authors: M Krzystek-K, B Szcz??niak, I Szczuka, P Fortuna, M Zawadzki, A Kubiak, M Mierzcha?a, MG Fleszar, ? Lewandowsk, P Serek, N Jamrozik, K Neubauer, J Wi?niewski, R Kempi?ski, W Witkiewicz, I Bednarz-Mi
    Cancers (Basel), 2020-09-11;12(9):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot

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