Human/Mouse/Rat 14-3-3 gamma Antibody

Catalog #: MAB5700
1 Citation Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB5700
MAB5700-SP

Detection of Human, Mouse, and Rat 14‑3‑3 gamma by Western Blot.

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All 14-3-3 gamma Antibodies

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Citations (1)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse/Rat 14-3-3 gamma Antibody Summary

Species Reactivity

Human, Mouse, Rat

Specificity

Detects human, mouse, and rat 14-3-3 gamma in Western blots.

Source

Monoclonal Mouse IgG_2B Clone # 505810

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human 14-3-3 gamma
Met1-Asn247
Accession # P61981

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human, Mouse, and Rat 14‑3‑3 gamma by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, NIH-3T3 mouse embryonic fibroblast cell line, and L6 rat myoblast cell line. PVDF membrane was probed with 1 µg/mL Mouse Anti-Human/Mouse/Rat 14-3-3 gamma Monoclonal Antibody (Catalog # MAB5700) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band for 14-3-3 gamma was detected at approximately 30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Western Blot

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Detection of Human 14‑3‑3 gamma by Western Blot. Western blot shows recombinant human 14-3-3 beta, epsilon, eta, gamma, sigma, theta, and zeta (5 ng/lane. PVDF membrane was probed with 1 µg/mL Mouse Anti-Human/Mouse/Rat 14-3-3 gamma Monoclonal Antibody (Catalog # MAB5700) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band for 14-3-3 gamma was detected at approximately 30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: 14-3-3 gamma

14-3-3 proteins are a highly conserved family of homo- and heterodimeric phosphoserine/threonine-binding proteins present in all eukaryotic cells. 14-3-3 proteins act as key regulators of intracellular signal transduction through their ability to bind specific motifs phosphorylated on serine or threonine. There are at least 7 distinct mammalian 14-3-3 genes: alpha / beta, epsilon, eta, gamma, tau / theta, sigma, and zeta /δ. 14-3-3 gamma becomes phosphorylated in response to PDGF and subsequently interacts with different PKC isoforms.

Entrez Gene IDs

7532 (Human); 22628 (Mouse); 56010 (Rat)

Alternate Names

1433 gamma; 14-3-3 gamma; 14-3-3 protein gamma; 14-3-3GAMMA; KCIP-1; Protein kinase C inhibitor protein 1; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, gammapolypeptide; YWHAG

Product Datasheets

Product Datasheet

COA

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Related Research Areas

Citation for Human/Mouse/Rat 14-3-3 gamma Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

YAP-dependent Wnt5a induction in hypertrophic adipocytes restrains adiposity
Authors: GJ Lee, YJ Kim, B Park, S Yim, C Park, H Roh, Y Moon, JK Seong, H Park
Cell Death & Disease, 2022-04-27;13(4):407.