Human/Mouse/Rat alpha -Smooth Muscle Actin Antibody
Human/Mouse/Rat alpha -Smooth Muscle Actin Antibody Summary
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
Detection of Human alpha ‑Smooth Muscle Actin by Western Blot. Western blot shows lysates of human heart tissue and iBJ6 human induced pluripotent stem cell line undifferentiated or cardiac differentiated. PVDF membrane was probed with 0.25 µg/mL of Mouse Anti-Human/ Mouse/Rat a-Smooth Muscle Actin Monoclonal Antibody (Catalog # MAB1420) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for a-Smooth Muscle Actin at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Mouse and Rat alpha ‑Smooth Muscle Actin by Western Blot. Western blot shows lysates of mouse colon tissue, C2C12 mouse myoblast cell line, NIH-3T3 mouse embryonic fibroblast cell line, and rat colon tissue. PVDF membrane was probed with 0.1 µg/mL of Mouse Anti-Human/Mouse/Rat a-Smooth Muscle Actin Monoclonal Antibody (Catalog # MAB1420) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for a-Smooth Muscle Actin at approximately 42 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
alpha ‑Smooth Muscle Actin in BG01V Human Embryonic Stem Cells. a-Smooth Muscle Actin was detected in immersion fixed BG01V human embryonic stem cells differentiated to cardiomyocytes using Mouse Anti-Human/Mouse/ Rat a-Smooth Muscle Actin Monoclonal Antibody (Catalog # MAB1420) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and cytoskeleton. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
alpha ‑Smooth Muscle Actin in Human Heart. a-Smooth Muscle Actin was detected in immersion fixed paraffin-embedded sections of human heart using Mouse Anti-Human/Mouse/Rat a-Smooth Muscle Actin Monoclonal Antibody (Catalog # MAB1420) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
alpha -Smooth Muscle Actin in Human Breast Cancer Tissue. a-Smooth Muscle Actin was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Mouse Anti-Human/ Mouse/Rat a-Smooth Muscle Actin Monoclonal Antibody (Catalog # MAB1420) at 8 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Detection of Human alpha ‑Smooth Muscle Actin by Simple WesternTM. Simple Western lane view shows lysates of iBJ6 human induced pluripotent stem cell line untreated (-) or differentiated to cardiomyocytes (+), loaded at 0.2 mg/mL. A specific band was detected for alpha ‑Smooth Muscle Actin at approximately 49 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human/Mouse/Rat alpha ‑Smooth Muscle Actin Monoclonal Antibody (Catalog # MAB1420). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Detection of alpha ‑Smooth Muscle Actin in Human Blood Monocytes by Flow Cytometry. Human peripheral blood monocytes were stained with Mouse Anti-Human/Mouse/Rat a-Smooth Muscle Actin Monoclonal Antibody (Catalog # MAB1420, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
alpha-Smooth Muscle Actin in Human Breast Cancer Tissue Using Dual RNAscope®ISH and IHC. alpha-Smooth Muscle Actin mRNA was detected (top image) in formalin-fixed paraffin-embedded tissue sections of human breast cancer tissue probed with ACD RNAScope® Probe (Catalog # 444771-C2) and stained using ACD RNAscope® 2.5 HD Duplex Detection Reagents (red, Catalog # 322500). Adjacent tissue section (bottom image) was processed for immunohistochemistry using R&D Systems Mouse Anti-Human/Mouse/Rat alpha-Smooth Muscle Actin Monoclonal Antibody (Catalog# MAB1420) at 0.5 ug/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte HRP Polymer Antibody (R&D Systems, Catalog # VC001) and DAB chromogen (brown). Tissues were counterstained with hematoxylin (blue).
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: alpha-Smooth Muscle Actin
alpha ‑Smooth Muscle Actin has been frequently used as a marker of smooth muscle differentiation (1, 2).
- Skalli, O. et al. (1986) J. Cell Biol. 103:2787.
- Oishi, K. et al. (2002) J. Physiol. 540:139.
Product Datasheets
Citations for Human/Mouse/Rat alpha -Smooth Muscle Actin Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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An integrated organoid omics map extends modeling potential of kidney disease
Authors: Lassé, M;El Saghir, J;Berthier, CC;Eddy, S;Fischer, M;Laufer, SD;Kylies, D;Hutzfeldt, A;Bonin, LL;Dumoulin, B;Menon, R;Vega-Warner, V;Eichinger, F;Alakwaa, F;Fermin, D;Billing, AM;Minakawa, A;McCown, PJ;Rose, MP;Godfrey, B;Meister, E;Wiech, T;Noriega, M;Chrysopoulou, M;Brandts, P;Ju, W;Reinhard, L;Hoxha, E;Grahammer, F;Lindenmeyer, MT;Huber, TB;Schlüter, H;Thiel, S;Mariani, LH;Puelles, VG;Braun, F;Kretzler, M;Demir, F;Harder, JL;Rinschen, MM;
Nature communications
Species: Human
Sample Types: Whole Cells
Applications: Immunocytochemistry/ Immunofluorescence -
An unbiased proteomics approach to identify the senescence-associated secretory phenotype of human bone marrow-derived mesenchymal stem cells
Authors: RM Samsonraj, SF Law, A Chandra, RJ Pignolo
Bone Reports, 2023-03-21;18(0):101674.
Species: Human
Sample Types: Cell Culture Supernates
Applications: Western Blot -
Co-culture models of endothelial cells, macrophages, and vascular smooth muscle cells for the study of the natural history of atherosclerosis
Authors: M Liu, S Samant, CH Vasa, RM Pedrigi, UM Oguz, S Ryu, T Wei, DR Anderson, DK Agrawal, YS Chatzizisi
PLoS ONE, 2023-01-20;18(1):e0280385.
Species: Human
Sample Types: Protein, Whole Cells
Applications: ICC, Western Blot -
Targeting Periostin Expression Makes Pancreatic Cancer Spheroids More Vulnerable to Natural Killer Cells
Authors: D Karakas, M Erkisa, RO Akar, G Akman, EY Senol, E Ulukaya
Biomedicines, 2023-01-19;11(2):.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Dietary oxidized frying oil activates hepatic stellate cells and accelerates the severity of carbon tetrachloride- and thioacetamide-induced liver fibrosis in mice
Authors: FM Suk, FY Hsu, YC Lee, TL Chen, WC Chiu, YJ Liao
The Journal of nutritional biochemistry, 2023-01-11;0(0):109267.
Species: Mouse
Sample Types: Cell Lysates
Applications: IHC, Western Blot -
CCL18 signaling from tumor-associated macrophages activates fibroblasts to adopt a chemoresistance-inducing phenotype
Authors: W Zeng, L Xiong, W Wu, S Li, J Liu, L Yang, L Lao, P Huang, M Zhang, H Chen, N Miao, Z Lin, Z Liu, X Yang, J Wang, P Wang, E Song, Y Yao, Y Nie, J Chen, D Huang
Oncogene, 2022-11-22;0(0):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Biomimetic nanoparticles drive the mechanism understanding of shear-wave elasticity stiffness in triple negative breast cancers to predict clinical treatment
Authors: D Zheng, J Zhou, L Qian, X Liu, C Chang, S Tang, H Zhang, S Zhou
Bioactive materials, 2022-11-03;22(0):567-587.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Alcohol and HIV-Derived Hepatocyte Apoptotic Bodies Induce Hepatic Stellate Cell Activation
Authors: M New-Aaron, RS Dagur, SS Koganti, M Ganesan, W Wang, E Makarov, M Ogunnaike, KK Kharbanda, LY Poluektova, NA Osna
Biology, 2022-07-14;11(7):.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Establishment of a human induced pluripotent stem cell line (TAUi008-A) derived from a multiple sclerosis patient
Authors: J Lotila, T Hyvärinen, H Skottman, L Airas, S Narkilahti, S Hagman
Oncogene, 2022-07-11;63(0):102865.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry, ICC -
AAV2-mediated gene therapy for Bietti crystalline dystrophy provides functional CYP4V2 in multiple relevant cell models
Authors: JH Wang, GE Lidgerwood, M Daniszewsk, ML Hu, GE Roberts, RCB Wong, SSC Hung, ME McClements, AW Hewitt, A Pébay, DG Hickey, TL Edwards
Oncogene, 2022-06-09;12(1):9525.
Species: Human
Sample Types: Whole Tissue
Applications: IHC -
Histochemical examination of blood vessels in murine femora with intermittent PTH administration
Authors: H Maruoka, S Zhao, H Yoshino, M Abe, T Yamamoto, H Hongo, M Haraguchi-, A Nasoori, H Ishizu, Y Nakajima, M Omaki, T Shimizu, N Iwasaki, PH Luiz de Fr, M Li, T Hasegawa
Journal of oral biosciences, 2022-05-15;0(0):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Myo5b Transports Fibronectin-Containing Vesicles and Facilitates FN1 Secretion from Human Pleural Mesothelial Cells
Authors: T Sakai, YY Choo, O Sato, R Ikebe, A Jeffers, S Idell, T Tucker, M Ikebe
International Journal of Molecular Sciences, 2022-04-27;23(9):.
Species: Human
Sample Types: Cell Culture Supernates
Applications: Western Blot -
Generation of a human induced pluripotent stem cell line derived from a patient with dilated cardiomyopathy carrying LMNA nonsense mutation
Authors: Y Shimoda, N Murakoshi, H Mori, D Xu, K Tajiri, Y Hemmi, I Sato, M Noguchi, Y Nakamura, Y Hayashi, M Ieda
Stem Cell Research, 2022-04-27;62(0):102793.
Species: Human
Sample Types: Spheroid
Applications: ICC -
Signature of the vascular tumor microenvironment as a marker of the therapeutic response to doxorubicin in a preclinical model of osteosarcoma
Authors: V Crenn, J Amiaud, A Gomez-Brou, V Potiron, F Gouin, P Rosset, LL Nail, L Vidal, H Bertin, R Brion, G Tran, F Verrecchia, I Corre, F Redini
American journal of cancer research, 2022-04-15;12(4):1843-1854.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
A vasculature niche orchestrates stromal cell phenotype through PDGF signaling: Importance in human fibrotic disease
Authors: TB Layton, L Williams, N Yang, M Zhang, C Lee, M Feldmann, G Trujillo, D Furniss, J Nanchahal
Proceedings of the National Academy of Sciences of the United States of America, 2022-03-23;119(13):e2120336119.
Species: Human
Sample Types: Whole Cells, Whole Tissue
Applications: CyTof, IHC -
CRISPR/Cas9-engineered human ES cells harboring heterozygous and homozygous c-KIT knockout
Authors: MAS de Toledo, X Fu, F Kluge, K Götz, S Schmitz, P Wanek, HM Schüler, K Pannen, N Chatain, S Koschmiede, TH Brümmendor, M Zenke
Stem Cell Research, 2022-03-01;60(0):102732.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Switching in the expression pattern of actin isoforms marks the onset of contractility and distinct mechanodynamic behavior during cardiomyocyte differentiation
Authors: RH Pires, TH Dau, E Manu, N Shree, O Otto
Physiological Reports, 2022-02-01;10(3):e15171.
Species: Human
Sample Types: Cell Lysates, Whole Cells
Applications: ICC, Western Blot -
Engagement of the CXCL12-CXCR4 Axis in the Interaction of Endothelial Progenitor Cell and Smooth Muscle Cell to Promote Phenotype Control and Guard Vascular Homeostasis
Authors: SF Mause, E Ritzel, A Deck, F Vogt, EA Liehn
International Journal of Molecular Sciences, 2022-01-14;23(2):.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Dermal Papilla Cell-Derived Extracellular Vesicles Increase Hair Inductive Gene Expression in Adipose Stem Cells via beta-Catenin Activation
Authors: T Kazi, A Nagata, T Nakagawa, T Matsuzaki, S Inui
Cells, 2022-01-07;11(2):.
Species: Mouse
Sample Types: Spheroids
Applications: ICC -
Cyclo(Val-Pro) and Cyclo(Leu-Hydroxy-Pro) from Pseudomonas sp. (ABS-36) alleviates acute and chronic renal injury under in vitro and in vivo models (Ischemic reperfusion and unilateral ureter obstruction)
Authors: K Hira, P Sharma, A Mahale, O Prakash Ku, A Sajeli Beg
International immunopharmacology, 2021-12-29;103(0):108494.
Species: Mouse, Rat
Sample Types: Cell Lysates, Whole Cells
Applications: ICC, Western Blot -
Generation of an induced pluripotent stem cell line GZHMCi008-A derived from a patient with SRY-positive 46,XX testicular disorder of sex development
Authors: Y Luo, Y Chen, M Zhang, X Ma, D Zhu, Y Chen
Stem Cell Research, 2021-10-22;57(0):102583.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry, ICC -
Synergistic effects of silver nanoparticles and cisplatin in combating inflammation and hyperplasia of airway stents
Authors: Z Li, C Tian, D Jiao, J Li, Y Li, X Zhou, H Zhao, Y Zhao, X Han
Bioactive materials, 2021-07-29;9(0):266-280.
Species: Rabbit
Sample Types: Whole Tissue
Applications: IHC -
Generation of three induced pluripotent stem cell lines, SCVIi003-A, SCVIi004-A, SCVIi005-A, from patients with ARVD/C caused by heterozygous mutations in the PKP2 gene
Authors: JWS Jahng, KE Black, L Liu, HR Bae, M Perez, EA Ashley, K Sallam, JC Wu
Stem Cell Research, 2021-03-12;53(0):102284.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry, ICC -
Cancer-associated fibroblast-derived SDF-1 induces epithelial-mesenchymal transition of lung adenocarcinoma via CXCR4/&beta-catenin/PPAR&delta signalling
Authors: Y Wang, W Lan, M Xu, J Song, J Mao, C Li, X Du, Y Jiang, E Li, R Zhang, Q Wang
Cell Death & Disease, 2021-02-26;12(2):214.
Species: Human
Sample Types: Whole Cells
Applications: ICC, Neutralization -
Cohort Analysis of ADAM8 Expression in the PDAC Tumor Stroma
Authors: C Jaworek, Y Verel-Yilm, S Driesch, S Ostgathe, L Cook, S Wagner, DK Bartsch, EP Slater, JW Bartsch
Journal of personalized medicine, 2021-02-10;11(2):.
Species: Human
Sample Types: Whole Tissue
Applications: IHC -
A non-invasive method to generate induced pluripotent stem cells from primate urine
Authors: J Geuder, LE Wange, A Janjic, J Radmer, P Janssen, JW Bagnoli, S Müller, A Kaul, M Ohnuki, W Enard
Scientific Reports, 2021-02-10;11(1):3516.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Generation of induced pluripotent stem cell (iPSC) lines carrying a heterozygous (UKWMPi002-A-1) and null mutant knockout (UKWMPi002-A-2) of Cadherin 13 associated with neurodevelopmental disorders using CRISPR/Cas9
Authors: MR Vitale, JEM Zöller, C Jansch, A Janz, F Edenhofer, E Klopocki, D van den Ho, T Vanmierlo, O Rivero, N Nadif Kasr, GC Ziegler, KP Lesch
Stem Cell Research, 2021-01-11;51(0):102169.
Species: Human
Sample Types: Cell Lysates, Whole Cells
Applications: ICC, Western Blot -
STRAP regulates alternative splicing fidelity during lineage commitment of mouse embryonic stem cells
Authors: L Jin, Y Chen, DK Crossman, A Datta, T Vu, JA Mobley, MK Basu, M Scarduzio, H Wang, C Chang, PK Datta
Nat Commun, 2020-11-23;11(1):5941.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Initiation of reticular and spider veins, incompetent perforantes and varicose veins in the saphenous vein network of the rat
Authors: BB Patai, G Dornyei, AM Tokes, JR Hetthessy, A Fees, GL Nadasy
Scientific Reports, 2020-09-21;10(1):15381.
Species: Rat
Sample Types: Whole Tissue
Applications: IHC -
SB772077B (SB77) Alleviated the Aqueous Outflow Resistance Mediated by Cyclic Mechanical Stress in Perfused Human Cadaveric Eyes
Authors: S Ashwinbala, R Haribalaga, S Krishnadas, V Muthukkaru, S Senthilkum
Sci Rep, 2020-06-23;10(1):10202.
Species: Human
Sample Types: Whole Tissues
Applications: IHC -
Acetyl-CoA Carboxylase inhibition improves multiple dimensions of NASH pathogenesis in model systems
Authors: TT Ross, C Crowley, KL Kelly, A Rinaldi, DA Beebe, MP Lech, RV Martinez, S Carvajal-G, M Boucher, D Hirenallur, J Morin, AC Opsahl, SR Vargas, KK Bence, JA Pfefferkor, WP Esler
Cell Mol Gastroenterol Hepatol, 2020-06-09;0(0):.
Species: Rat
Sample Types: Whole Tissues
Applications: IHC -
Reconstruction of the human blood-brain barrier in vitro reveals a pathogenic mechanism of APOE4 in pericytes
Authors: JW Blanchard, M Bula, J Davila-Vel, LA Akay, L Zhu, A Frank, MB Victor, JM Bonner, H Mathys, YT Lin, T Ko, DA Bennett, HP Cam, M Kellis, LH Tsai
Nat. Med., 2020-06-08;26(6):952-963.
Species: Human
Sample Types: Cell Lysates, Whole Cells
Applications: ICC, Western Blot -
Cellular census of human fibrosis defines functionally distinct stromal cell types and states
Authors: TB Layton, L Williams, F McCann, M Zhang, M Fritzsche, H Colin-York, M Cabrita, MTH Ng, M Feldmann, SN Sansom, D Furniss, W Xie, J Nanchahal
Nat Commun, 2020-06-02;11(1):2768.
Species: Human
Sample Types: Whole Cells
Applications: CyTof -
CD8+ T-cell plasticity regulates vascular regeneration in type-2 diabetes
Authors: C Liang, KY Yang, VW Chan, X Li, THW Fung, Y Wu, XY Tian, Y Huang, L Qin, JYW Lau, KO Lui
Theranostics, 2020-03-04;10(9):4217-4232.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Dynamic Changes in Function and Proteomic Composition of Extracellular Vesicles from Hepatic Stellate Cells during Cellular Activation
Authors: X Li, R Chen, S Kemper, DR Brigstock
Cells, 2020-01-25;9(2):.
Species: Mouse
Sample Types: Whole Cells
Applications: ICC -
Optimal stimulation toward the dermal papilla lineage can be promoted by combined use of osteogenic and adipogenic inducers
Authors: T Kazi, I Niibe, A Nishikawa, T Matsuzaki
FEBS Open Bio, 2019-12-28;0(0):.
Species: Mouse
Sample Types: Whole Cells
Applications: ICC -
Empagliflozin reduces high glucose-induced oxidative stress and miR-21-dependent TRAF3IP2 induction and RECK suppression, and inhibits human renal proximal tubular epithelial cell migration and epithelial-to-mesenchymal transition
Authors: NA Das, AJ Carpenter, A Belenchia, AR Aroor, M Noda, U Siebenlist, B Chandrasek, VG DeMarco
Cell. Signal., 2019-12-17;68(0):109506.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Fibroblast-like synovial cell production of extra domain A fibronectin associates with inflammation in osteoarthritis
Authors: TW Kragstrup, DH Sohn, CM Lepus, K Onuma, Q Wang, WH Robinson, J Sokolove
BMC Rheumatol, 2019-12-02;3(0):46.
Species: Human
Sample Types: Whole Cells, Whole Tissue
Applications: ICC, IHC-Fr -
Adipose Mesenchymal Cells-Derived EVs Alleviate DOCA-Salt-Induced Hypertension by Promoting Cardio-Renal Protection
Authors: RS Lindoso, JA Lopes, R Binato, E Abdelhay, CM Takiya, KR Miranda, LS Lara, A Viola, B Bussolati, A Vieyra, F Collino
Mol Ther Methods Clin Dev, 2019-11-15;16(0):63-77.
Species: Rat
Sample Types: Tissue Homogenates
Applications: Western Blot -
Pathological Study on Epithelial-Mesenchymal Transition in Silicotic Lung Lesions in Rat
Authors: M Komai, K Mihira, A Shimada, I Miyamoto, K Ogihara, Y Naya, T Morita, K Inoue, H Takano
Vet Sci, 2019-08-30;6(3):.
Species: Rat
Sample Types: Tissue Homogenates, Whole Tissue
Applications: IHC-P, Western Blot -
Human induced pluripotent stem cell line HMUi001-A derived from corneal stromal cells
Authors: T Bikkuzin, Y Shi, B Sun, Y Guo, X Jin, Z Han, V Pavlov, H Zhang
Stem Cell Res, 2019-02-15;37(0):101409.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Single-cell analysis reveals fibroblast heterogeneity and myeloid-derived adipocyte progenitors in murine skin wounds
Authors: CF Guerrero-J, PH Dedhia, S Jin, R Ruiz-Vega, D Ma, Y Liu, K Yamaga, O Shestova, DL Gay, Z Yang, K Kessenbroc, Q Nie, WS Pear, G Cotsarelis, MV Plikus
Nat Commun, 2019-02-08;10(1):650.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-P -
Inhibition of PAI-1 limits chemotherapy resistance in lung cancer through suppressing myofibroblast characteristics of cancer-associated fibroblasts
Authors: T Masuda, T Nakashima, M Namba, K Yamaguchi, S Sakamoto, Y Horimasu, S Miyamoto, H Iwamoto, K Fujitaka, Y Miyata, H Hamada, M Okada, N Hattori
J. Cell. Mol. Med., 2019-02-07;0(0):.
Species: Human
Sample Types: Whole Tissue
Applications: IHC -
TGF-?-SNAIL axis induces M�ller glial-mesenchymal transition in the pathogenesis of idiopathic epiretinal membrane
Authors: A Kanda, K Noda, I Hirose, S Ishida
Sci Rep, 2019-01-24;9(1):673.
Species: Human
Sample Types: Cell Lysates, Whole Cells
Applications: ICC, Western Blot -
Small non-coding RNA landscape of extracellular vesicles from human stem cells
Authors: S Kaur, AG Abu-Shahba, RO Paananen, H Hongisto, H Hiidenmaa, H Skottman, R Seppänen-K, B Mannerströ
Sci Rep, 2018-10-19;8(1):15503.
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Ebola Virus Causes Intestinal Tract Architectural Disruption and Bacterial Invasion in Non-Human Primates
Authors: RB Reisler, X Zeng, CW Schellhase, JJ Bearss, TK Warren, JC Trefry, GW Christophe, MG Kortepeter, S Bavari, AP Cardile
Viruses, 2018-09-20;10(10):.
Applications: IHC-P -
Exosomal miR-21-5p derived from gastric cancer promotes peritoneal metastasis via mesothelial-to-mesenchymal transition
Authors: Q Li, B Li, Q Li, S Wei, Z He, X Huang, L Wang, Y Xia, Z Xu, Z Li, W Wang, L Yang, D Zhang, Z Xu
Cell Death Dis, 2018-08-28;9(9):854.
Species: Human
Sample Types: Tissue Homogenates, Whole Tissue
Applications: IHC, Western Blot -
Modeling the neuropsychiatric manifestations of Lowe syndrome using induced pluripotent stem cells: defective F-actin polymerization and WAVE-1 expression in neuronal cells
Authors: J Barnes, F Salas, R Mokhtari, H Dolstra, E Pedrosa, HM Lachman
Mol Autism, 2018-08-15;9(0):44.
Species: Human
Sample Types: Organoids
Applications: IHC -
Implication of molecular vascular smooth muscle cell heterogeneity among arterial beds in arterial calcification
Authors: O Espitia, M Chatelais, M Steenman, C Charrier, B Maurel, S Georges, R Houlgatte, F Verrecchia, B Ory, F Lamoureux, D Heymann, Y Gouëffic, T Quillard
PLoS ONE, 2018-01-26;13(1):e0191976.
Species: Human
Sample Types: Whole Tissue
Applications: IHC -
Establishment and characterization of five immortalized human scalp dermal papilla cell lines
Authors: MH Kwack, JM Yang, GH Won, MK Kim, JC Kim, YK Sung
Biochem. Biophys. Res. Commun., 2018-01-10;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Wnt inhibition promotes vascular specification of embryonic cardiac progenitors
Authors: DE Reichman, L Park, L Man, D Redmond, K Chao, RP Harvey, MM Taketo, Z Rosenwaks, D James
Development, 2018-01-08;145(1):.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Buyang Huanwu Tang improves denervation-dependent muscle atrophy by increasing ANGPTL4, and increases NF-?B and MURF1 levels
Authors: L Zhou, Y Huang, H Xie, X Mei
Mol Med Rep, 2017-12-19;0(0):.
Species: Rat
Sample Types: Tissue Homogenates
Applications: Western Blot -
P2X7 receptor antagonism modulates IL-1? and MMP9 in human atherosclerotic vessels
Authors: M Lombardi, ME Mantione, D Baccellier, D Ferrara, R Castellano, R Chiesa, O Alfieri, C Foglieni
Sci Rep, 2017-07-07;7(1):4872.
Species: Human
Sample Types: Whole Tissue
Applications: IHC -
KIF5A transports collagen vesicles of myofibroblasts during pleural fibrosis
Authors: H Kamata, Y Tsukasaki, T Sakai, R Ikebe, J Wang, A Jeffers, J Boren, S Owens, T Suzuki, M Higashihar, S Idell, TA Tucker, M Ikebe
Sci Rep, 2017-07-04;7(1):4556.
Species: Human
Sample Types: Cell Lysates, Whole Tissue
Applications: IHC, Western Blot -
Generation of a human induced pluripotent stem cell line CERAi001-A-6 using episomal vectors
Authors: RCB Wong, SS Hung, S Jackson, V Singh, S Khan, HH Liang, LS Kearns, T Nguyen, A Conquest, M Daniszewsk, AW Hewitt, A Pébay
Stem Cell Res, 2017-05-19;22(0):13-15.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Mitochondrial replacement in an iPSC model of Leber's hereditary optic neuropathy
Authors: RCB Wong, SY Lim, SSC Hung, S Jackson, S Khan, NJ Van Bergen, E De Smit, HH Liang, LS Kearns, L Clarke, DA Mackey, AW Hewitt, IA Trounce, A Pébay
Aging (Albany NY), 2017-04-01;9(4):1341-1350.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Cancer-associated mesenchymal stroma fosters the stemness of osteosarcoma cells in response to intratumoral acidosis via NF-?B acivation
Authors: Sofia Avnet
Int. J. Cancer, 2017-03-15;0(0):.
Species: Canine
Sample Types: Whole Tissue
Applications: IHC -
Pathologic Roles of Receptor-Associated Prorenin System in Idiopathic Epiretinal Membrane
Authors: Y Dong, A Kanda, K Noda, W Saito, S Ishida
Sci Rep, 2017-03-09;7(0):44266.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P -
NOTCH1-dependent nitric oxide signaling deficiency in hypoplastic left heart syndrome revealed through patient-specific phenotypes detected in bioengineered cardiogenesis
Authors: SC Hrstka, X Li, TJ Nelson
Stem Cells, 2017-03-05;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Cancer-associated fibroblasts promote an immunosuppressive microenvironment through the induction and accumulation of protumoral macrophages
Authors: H Takahashi, K Sakakura, T Kudo, M Toyoda, K Kaira, T Oyama, K Chikamatsu
Oncotarget, 2017-01-31;8(5):8633-8647.
Species: Human
Sample Types: Whole Tissue
Applications: IHC -
Neuropathogenesis of Zika Virus in a Highly Susceptible Immunocompetent Mouse Model after Antibody Blockade of Type I Interferon
Authors: DR Smith, B Hollidge, S Daye, X Zeng, C Blancett, K Kuszpit, T Bocan, JW Koehler, S Coyne, T Minogue, T Kenny, X Chi, S Yim, L Miller, C Schmaljohn, S Bavari, JW Golden
PLoS Negl Trop Dis, 2017-01-09;11(1):e0005296.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-P -
Human pancreatic cancer cells induce a MyD88-dependent stromal response to promote a tumor-tolerant immune microenvironment
Cancer Res., 2016-11-18;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry, ICC -
The influence of warm ischemia elimination on kidney injury during transplantation - clinical and molecular study
Sci Rep, 2016-11-03;6(0):36118.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P -
Generation of Induced Cardiospheres via Reprogramming of Skin Fibroblasts for Myocardial Regeneration
Authors: Jian-Yong Xu
Stem Cells, 2016-07-07;0(0):.
Species: Mouse
Sample Types: Whole Cells
Applications: IHC -
Exosomes in Human Breast Milk Promote EMT
Authors: W Qin, Y Tsukasaki, S Dasgupta, N Mukhopadhy, M Ikebe, ER Sauter
Clin Cancer Res, 2016-04-08;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: IHC -
Automated Analysis and Classification of Histological Tissue Features by Multi-Dimensional Microscopic Molecular Profiling.
Authors: Riordan D, Varma S, West R, Brown P
PLoS ONE, 2015-07-15;10(7):e0128975.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P -
CD105 expression on CD34-negative spindle-shaped stromal cells of primary tumor is an unfavorable prognostic marker in early breast cancer patients.
Authors: Martinez L, Labovsky V, Calcagno M, Davies K, Garcia Rivello H, Bianchi M, Wernicke A, Fernandez Vallone V, Chasseing N
PLoS ONE, 2015-03-24;10(3):e0121421.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P -
Establishment and characterization of an immortalized human hepatic stellate cell line for applications in co-culturing with immortalized human hepatocytes.
Authors: Pan X, Wang Y, Yu X, Li J, Zhou N, Du W, Zhang Y, Cao H, Zhu D, Chen Y, Li L
Int J Med Sci, 2015-02-08;12(3):248-55.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Osteoprotegerin, pericytes and bone-like vascular calcification are associated with carotid plaque stability.
Authors: Davaine J, Quillard T, Brion R, Laperine O, Guyomarch B, Merlini T, Chatelais M, Guilbaud F, Brennan M, Charrier C, Heymann D, Goueffic Y, Heymann M
PLoS ONE, 2014-09-26;9(9):e107642.
Species: Human
Sample Types: Whole Tissue
Applications: IHC -
Association between PON1 rs662 polymorphism and coronary artery disease.
Authors: Liu T, Zhang X, Zhang J, Liang Z, Cai W, Huang M, Yan C, Zhu Z, Han Y
Eur J Clin Nutr, 2014-06-11;68(9):1029-35.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P -
Efficient generation of integration-free human induced pluripotent stem cells from keratinocytes by simple transfection of episomal vectors.
Authors: Piao Y, Hung S, Lim S, Wong R, Ko M
Stem Cells Transl Med, 2014-06-05;3(0):787-791.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Occurrence of tertiary lymphoid tissue is associated with T-cell infiltration and predicts better prognosis in early-stage colorectal cancers.
Authors: Di Caro G, Bergomas F, Grizzi F, Doni A, Bianchi P, Malesci A, Laghi L, Allavena P, Mantovani A, Marchesi F
Clin Cancer Res, 2014-02-12;20(8):2147-58.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P -
Quaking, an RNA-binding protein, is a critical regulator of vascular smooth muscle cell phenotype.
Authors: van der Veer E, de Bruin R, Kraaijeveld A, de Vries M, Bot I, Pera T, Segers F, Trompet S, van Gils J, Roeten M, Beckers C, van Santbrink P, Janssen A, van Solingen C, Swildens J, de Boer H, Peters E, Bijkerk R, Rousch M, Doop M, Kuiper J, Schalij M, van der Wal A, Richard S, van Berkel T, Pickering J, Hiemstra P, Goumans M, Rabelink T, de Vries A, Quax P, Jukema J, Biessen E, van Zonneveld A
Circ Res, 2013-08-20;113(9):1065-75.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
Transcriptional effects of 1,25 dihydroxyvitamin D(3) physiological and supra-physiological concentrations in breast cancer organotypic culture.
Authors: Milani C, Katayama M, de Lyra E, Welsh J, Campos L, Brentani M, Maciel M, Roela R, del Valle P, Goes J, Nonogaki S, Tamura R, Folgueira M
BMC Cancer, 2013-03-15;13(0):119.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Distinct transcriptional profiles of angioblasts derived from human embryonic stem cells.
Authors: Song S, Jung W, Kim K, Hong W, Kim H, Lee K, Lee K, Suh W
Exp Cell Res, 2013-02-28;319(8):1136-45.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Locally different endothelial nitric oxide synthase protein levels in ascending aortic aneurysms of bicuspid and tricuspid aortic valve.
Authors: Mohamed SA, Radtke A, Saraei R
Cardiol Res Pract, 2012-06-18;2012(0):165957.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Inhibition of caspase-3 differentially affects vascular smooth muscle cell apoptosis in the concave versus convex aortic sites in ascending aneurysms with a bicuspid aortic valve.
Authors: Mohamed SA, Misfeld M, Hanke T, Charitos EI, Bullerdiek J, Belge G, Kuehnel W, Sievers HH
Ann. Anat., 2010-04-11;192(3):145-50.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Effect of an antimicrobial agent on atherosclerotic plaques: assessment of metalloproteinase activity by molecular imaging.
Authors: Ohshima S, Fujimoto S, Petrov A, Nakagami H, Haider N, Zhou J, Tahara N, Osako MK, Fujimoto A, Zhu J, Murohara T, Edwards DS, Narula N, Wong ND, Chandrashekhar Y, Morishita R, Narula J
J. Am. Coll. Cardiol., 2010-03-23;55(12):1240-9.
Species: Rabbit
Sample Types: Whole Tissue
Applications: IHC-P -
Lysosome-dependent Ca(2+) release response to Fas activation in coronary arterial myocytes through NAADP: evidence from CD38 gene knockouts.
Authors: Zhang F, Xia M, Li PL
Am. J. Physiol., Cell Physiol., 2010-03-03;298(5):C1209-16.
Species: Mouse
Sample Types: Whole Cells
Applications: ICC -
The effect of VEGF on the myogenic differentiation of adipose tissue derived stem cells within thermosensitive hydrogel matrices.
Authors: Kim MH, Hong HN, Hong JP, Park CJ, Kwon SW, Kim SH, Kang G, Kim M
Biomaterials, 2009-11-14;31(6):1213-8.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Hypoxia-induced alveolar epithelial-mesenchymal transition requires mitochondrial ROS and hypoxia-inducible factor 1.
Authors: Zhou G, Dada LA, Wu M, Kelly A, Trejo H, Zhou Q, Varga J, Sznajder JI
Am. J. Physiol. Lung Cell Mol. Physiol., 2009-10-02;297(6):L1120-30.
Species: Rat
Sample Types: Whole Cells
Applications: ICC -
Molecular imaging of matrix metalloproteinase in atherosclerotic lesions: resolution with dietary modification and statin therapy.
Authors: Fujimoto S, Hartung D, Ohshima S, Edwards DS, Zhou J, Yalamanchili P, Azure M, Fujimoto A, Isobe S, Matsumoto Y, Boersma H, Wong N, Yamazaki J, Narula N, Petrov A, Narula J
J. Am. Coll. Cardiol., 2008-12-02;52(23):1847-57.
Species: Rabbit
Sample Types: Whole Tissue
Applications: IHC -
Role of matrix metalloprotease-9 in hyperoxic injury in developing lung.
Authors: Chetty A, Cao GJ, Severgnini M, Simon A, Warburton R, Nielsen HC
Am. J. Physiol. Lung Cell Mol. Physiol., 2008-07-25;295(4):L584-92.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot -
Breast carcinoma-associated fibroblasts and their counterparts display neoplastic-specific changes.
Authors: Hawsawi NM, Ghebeh H, Hendrayani SF, Tulbah A, Al-Eid M, Al-Tweigeri T, Ajarim D, Alaiya A, Dermime S, Aboussekhra A
Cancer Res., 2008-04-15;68(8):2717-25.
Species: Human
Sample Types: Whole Tissue
Applications: IHC -
Gadodiamide contrast agent 'activates' fibroblasts: a possible cause of nephrogenic systemic fibrosis.
Authors: Edward M, Quinn JA, Mukherjee S, Jensen MB, Jardine AG, Mark PB, Burden AD
J. Pathol., 2008-04-01;214(5):584-93.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Differentiation of human circulating fibrocytes as mediated by transforming growth factor-beta and peroxisome proliferator-activated receptor gamma.
Authors: Hong KM, Belperio JA, Keane MP, Burdick MD, Strieter RM
J. Biol. Chem., 2007-06-07;282(31):22910-20.
Species: Human
Sample Types: Cell Lysates, Whole Cells
Applications: ICC, Western Blot -
Bronchoalveolar lavage fluid from patients with acute lung injury/acute respiratory distress syndrome induces myofibroblast differentiation.
Authors: Synenki L, Chandel NS, Budinger GR, Donnelly HK, Topin J, Eisenbart J, Jovanovic B, Jain M
Crit. Care Med., 2007-03-01;35(3):842-8.
Species: Human
Sample Types: Whole Cells
Applications: ICC
FAQs
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Human/Mouse/Rat alpha-Smooth Muscle Actin Antibody, Catalog # MAB1420, is validated for Human/Mouse/Rat IHC, but the datasheet only has Human IHC examples. Was there testing on Mouse and Rat IHC samples?
Mouse and Rat samples were tested in Western Blot. We did not perform testing with Mouse or Rat IHC samples, however there are several citations for IHC using Mouse and Rat tissue on our webpage.
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Average Rating: 4.9 (Based on 8 Reviews)
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