Human/Mouse/Rat Annexin A1 Antibody Summary
Met1-Asn346
Accession # P04083
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human, Mouse, and Rat Annexin A1 by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line, NIH-3T3 mouse embryonic fibroblast cell line, and L6 rat myoblast cell line. PVDF membrane was probed with 0.1 µg/mL of Mouse Anti-Human/Mouse Annexin A1 Monoclonal Antibody (Catalog # MAB37701) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Annexin A1 at approximately 39 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
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Detection of Human Annexin A1 by Simple WesternTM. Simple Western lane view shows lysates of Jurkat human acute T cell leukemia cell line, loaded at 0.2 mg/mL. A specific band was detected for Annexin A1 at approximately 45 kDa (as indicated) using 1 µg/mL of Mouse Anti-Human/Mouse/Rat Annexin A1 Monoclonal Antibody (Catalog # MAB37701). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
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Detection of Mouse Annexin A1 by Simple WesternTM. Simple Western lane view shows lysates of NIH‑3T3 mouse embryonic fibroblast cell line, loaded at 0.2 mg/mL. A specific band was detected for Annexin A1 at approximately 45 kDa (as indicated) using 1 µg/mL of Mouse Anti-Human/Mouse/Rat Annexin A1 Monoclonal Antibody (Catalog # MAB37701). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
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Western Blot Shows Mouse Annexin A1 Specificity by Using Knockout Cell Line. Western blot shows lysates of NIH-3T3 mouse embryonic fibroblast parental cell line and Annexin A1 knockout NIH-3T3 cell line (KO). PVDF membrane was probed with 0.1 µg/mL of Mouse Anti-Human/Mouse/Rat Annexin A1 Monoclonal Antibody (Catalog # MAB37701) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Annexin A1 at approximately 38 kDa (as indicated) in the parental NIH-3T3 cell line, but is not detectable in knockout NIH-3T3 cell line. GAPDH (Catalog # MAB5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Annexin A1
The Annexins are a family of Calcium-dependent phospholipid-binding proteins that are preferentially located on the cytosolic face of the plasma membrane. The Annexins have a molecular weight of approximately 35 to 40 kDa and consist of a unique amino terminal domain followed by a homologous C-terminal core domain containing the calcium-dependent phospholipid-binding sites. The C‑terminal domain is comprised of four 60‑70 amino acid repeats, known as annexin repeats or an endonexin fold (Annexin A6 contains 8 annexin repeats). The four annexin repeats form a highly alpha -helical, tightly packed disc known as the annexin domain, which binds to phospholipids in the membrane in a calcium-dependent manner. Members of the annexin family play a role in cytoskeletal interactions, phospholipase inhibition, regulation of cellular growth, and intracellular signal transduction pathways. Annexin A1 (ANXA1), also known as annexin I, lipocortin I, and calpactin II, is an ~ 40 kDa protein with phospholipase A2 inhibitory activity. Since phospholipase A2 is required for the biosynthesis of the potent mediators of inflammation, prostaglandins and leukotrienes, Annexin A1 may have anti-inflammatory activity. Human Annexin A1 shares 88 and 89% amino acid sequence identity with mouse and rat Annexin A1, respectively.
Product Datasheets
Citations for Human/Mouse/Rat Annexin A1 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 3
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Extracellular vesicles produced by irradiated endothelial or Glioblastoma stem cells promote tumor growth and vascularization modulating tumor microenvironment
Authors: Castellani, G;Buccarelli, M;D'Alessandris, QG;Ilari, R;Cappannini, A;Pedini, F;Boe, A;Lulli, V;Parolini, I;Giannetti, S;Biffoni, M;Zappavigna, V;Marziali, G;Pallini, R;Ricci-Vitiani, L;
Cancer cell international
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
Phosphatidylserine-Exposing Annexin A1-Positive Extracellular Vesicles: Potential Cancer Biomarkers
Authors: GI Perez, MP Bernard, D Vocelle, AA Zarea, NA Saleh, MA Gagea, D Schneider, M Bauzon, T Hermiston, M Kanada
Vaccines, 2023-03-13;11(3):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Upregulation of annexin A1 protein expression in the intratumoral vasculature of human non-small-cell lung carcinoma and rodent tumor models
Authors: KL Allen, J Cann, W Zhao, N Peterson, M Lazzaro, H Zhong, H Wu, WF Dall'Acqua, MJ Borrok, MM Damschrode, P Tsui, Q Li
PLoS ONE, 2020-06-04;15(6):e0234268.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
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