Human/Mouse/Rat Bcl-x_L Antibody

Catalog #: MAB894
1 Citation Datasheet / COA / SDS

Recombinant Monoclonal Antibody

Catalog #	Availability	Size / Price	Qty
MAB894-SP
MAB894-100

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Citations (1)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse/Rat Bcl-x_L Antibody Summary

Species Reactivity

Human, Mouse, Rat

Specificity

Detects human Bcl‑x_L in direct ELISAs and detects human, mouse, and rat Bcl‑x_L in Western blots.

Source

Recombinant Monoclonal Rabbit IgG Clone # 2052A

Purification

Protein A or G purified from cell culture supernatant

Immunogen

E. coli-derived recombinant human Bcl‑x_L
Met1-Arg212
Accession # Q07817

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.5 µg/mL

See below

Simple Western

5 µg/mL

See below

Immunohistochemistry

Immersion fixed paraffin-embedded sections of Human Prostate

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Bcl‑x_Lby Western Blot. Western blot shows lysates of K562 human chronic myelogenous leukemia cell line, Jurkat human acute T cell leukemia cell line, C6 rat glioma cell line, and RAW 264.7 mouse monocyte/macrophage cell line. PVDF membrane was probed with 0.5 µg/mL of Rabbit Anti-Human/Mouse/Rat Bcl-x_LMonoclonal Antibody (Catalog # MAB894) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (HAF008). A specific band was detected for Bcl-x_Lat approximately 25 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western

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Detection of Human Bcl‑x_Lby Simple Western^TM. Simple Western lane view shows lysates of K562 human chronic myelogenous leukemia cell line and NIH‑3T3 mouse embryonic fibroblast cell line, loaded at 0.2 mg/mL. A specific band was detected for Bcl‑x_Lat approximately 33 kDa (as indicated) using 5 µg/mL of Rabbit Anti-Human/Mouse/Rat Bcl‑x_LMonoclonal Antibody (Catalog # MAB894). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Immunohistochemistry

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Detection of Bcl‑x_L in Human Prostate. Bcl‑x_L was detected in immersion fixed paraffin-embedded sections of Human Prostate using Rabbit Anti-Human/Mouse/Rat Bcl‑x_L Monoclonal Antibody (Catalog # MAB894) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC003). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to secretetory vesicles in epithelial cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Bcl-xL

Bcl-x_L is a member of the Bcl-2 family of proteins that regulates outer mitochondrial membrane permeability (1, 2). Bcl-x_L is an anti-apoptotic member that prevents release of cytochrome c from the mitochondria intermembrane space into the cytosol. Bcl-x_L is present on the outer mitochondrial membrane and is also found on other membranes in some cell types. Natural Bcl-x_L contains a carboxyl-terminal mitochondria targeting sequence. Recombinant Bcl-x_L missing the mitochondrial targeting sequence maintains its ability to neutralize pro-apoptotic Bcl-2 family members. Neutralization by Bcl-x_L appears to be mediated through binding the BH3 region of pro-apoptotic Bcl-2 family members. This activity does not require the mitochondrial targeting sequence.

References

Gross, A. et al. (1999) Genes and Develop. 13:1899.
Kroemer, G. (1997) Nature Med. 3:614.

Long Name

B Cell Lymphoma/Leukemia x Long Form

Entrez Gene IDs

598 (Human); 12048 (Mouse); 24888 (Rat)

Alternate Names

Apoptosis regulator Bcl-X; bcl2-L-1; BCL2-like 1; bcl-2-like protein 1; BCLXBCL2LBcl-X; BclxL; Bcl-xL; BCL-XL/S; BCLXS; bcl-xS; DKFZp781P2092

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citation for Human/Mouse/Rat Bcl-x_L Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

The novel TRAIL-receptor agonist APG350 exerts superior therapeutic activity in pancreatic cancer cells
Authors: K Legler, C Hauser, JH Egberts, A Willms, C Heneweer, S Boretius, C Röcken, CC Glüer, T Becker, M Kluge, O Hill, C Gieffers, H Fricke, H Kalthoff, J Lemke, A Trauzold
Cell Death Dis, 2018-05-01;9(5):445.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot