Human/Mouse/Rat Beclin 1 Antibody Summary
no cross-reactivity with recombinant human ATG3, 4A, 4B, 5, 7, 10, 12, or 16L1 is observed.
Met12-Gln239
Accession # Q14457
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human, Mouse, and Rat Beclin 1 by Western Blot. Western blot shows lysates of A431 human epithelial carcinoma cell line, A20 mouse B cell lymphoma cell line, and NRK rat normal kidney cell line. PVDF Membrane was probed with 0.1 µg/mL of Mouse Anti-Human Beclin 1 Monoclonal Antibody (Catalog # MAB5295) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF007). A specific band was detected for Beclin 1 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
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Detection of Human Beclin 1 by Simple WesternTM. Simple Western lane view shows lysates of human brain (cerebellum) tissue, loaded at 0.2 mg/mL. A specific band was detected for Beclin 1 at approximately 58 kDa (as indicated) using 1 µg/mL of Mouse Anti-Human/Mouse/Rat Beclin 1 Monoclonal Antibody (Catalog # MAB5295). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
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Detection of Mouse Beclin 1 by Simple WesternTM. Simple Western lane view shows lysates of mouse brain tissue, loaded at 0.2 mg/mL. A specific band was detected for Beclin 1 at approximately 58 kDa (as indicated) using 1 µg/mL of Mouse Anti-Human/Mouse/Rat Beclin 1 Monoclonal Antibody (Catalog # MAB5295). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
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Detection of Beclin 1 in Human Kidney. Beclin 1 was detected in immersion fixed paraffin-embedded sections of Human Kidney using Mouse Anti-Human/Mouse/Rat Beclin 1 Monoclonal Antibody (Catalog # MAB5295) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to glomeruli. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Beclin 1
Beclin 1 (Bcl-2 interacting coiled-coil protein; also know as ATG6) is a 60 kDa member of the Beclin family of proteins. It is part of a lipid-kinase complex that includes UVRAG and PI3 and induces autophagy; in the presence of Bcl-2, autophagy is repressed. Beclin 1 is widely expressed but abundantly found in mammary epithelium, glia and neurons. Human Beclin 1 is 450 amino acids (aa) in length and contains a Bcl-2 binding domain (aa 88‑150), a nuclear export signal motif (aa 180‑189), a coiled-coil region (aa 142‑270), and an evolutionary conserved domain (aa 244‑337). There is one potential alternate start site at Met88. Over aa 12‑239, human Beclin 1 is 98% aa identical to mouse Beclin 1.
Product Datasheets
Citations for Human/Mouse/Rat Beclin 1 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 3
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Calcitriol Inhibits Proliferation and Potentially Induces Apoptosis in B16-F10 Cells
Authors: Eva Krishna Sutedja, Daniar Amarassaphira, Hanna Goenawan, Yuni Susanti Susanti Pratiwi, Nova Sylviana, Budi Setiabudiawan et al.
Medical Science Monitor Basic Research
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GK-1 Induces Oxidative Stress, Mitochondrial Dysfunction, Decreased Membrane Potential, and Impaired Autophagy Flux in a Mouse Model of Breast Cancer
Authors: Alfredo Cruz-Gregorio, Ana Karina Aranda-Rivera, Omar Emiliano Aparicio-Trejo, Omar Noel Medina-Campos, Edda Sciutto, Gladis Fragoso et al.
Antioxidants (Basel)
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Bupivacaine at clinically relevant concentrations induces toxicity in human intervertebral disc cells via the induction of autophagy in vitro
Authors: G Yang, Z Li, H Mei, W Ye, S Huang, K Liu, Q Tan
Mol Med Rep, 2019-05-22;0(0):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
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