Human/Mouse/Rat beta-Catenin Antibody

Name: Human/Mouse/Rat beta-Catenin Antibody
Brand: R&D Systems
SKU: MAB1329
Rating: 3.5 (2 reviews)

Catalog #: MAB1329
4 Citations 2 Reviews Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB1329-050
MAB1329-200
MAB1329-SP

Detection of Human/Mouse/Rat beta -Catenin by Western Blot.

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Product Details

Citations (4)

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Reviews (2)

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse/Rat beta-Catenin Antibody Summary

Species Reactivity

Human, Mouse, Rat

Specificity

Detects rh beta -Catenin and endogenous human, mouse and rat beta -Catenin in Western blots.

Source

Monoclonal Mouse IgG_2B Clone # 196621

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human beta -Catenin
Ala2-Leu781
Accession # P35222

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Purity

Protein A or G purified from hybridoma culture supernatant

Applications

Recommended Concentration

Sample

Western Blot

2 µg/mL

See below

Immunohistochemistry

8-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human/Mouse/Rat beta -Catenin by Western Blot. Western blot shows lysates of Huh-7 human hepatoma cell line, C6 rat glioma cell line, and NIH-3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human/Mouse/Rat beta -Catenin Monoclonal Antibody (Catalog # MAB1329) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for beta -Catenin at approximately 95 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.

Immunohistochemistry

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beta ‑Catenin in Human Pancreas. beta -Catenin was detected in immersion fixed paraffin-embedded sections of human pancreas using Mouse Anti-Human/Mouse/Rat beta -Catenin Monoclonal Antibody (Catalog # MAB1329) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counter-stained with hematoxylin (blue). Specific staining was localized to plasma membranes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: beta-Catenin

beta -Catenin is a cadherin-associated protein that is involved in the regulation of cell adhesion. It also acts as a transcriptional co-activator in the nucleus and is involved in the canonical Wnt signal transduction pathway.

Entrez Gene IDs

1499 (Human); 12387 (Mouse); 84353 (Rat)

Alternate Names

bCatenin; beta 1 (88kD); beta-Catenin; catenin (cadherin-associated protein), beta 1, 88kDa; catenin beta-1; CTNNB; CTNNB1; DKFZp686D02253; FLJ25606; FLJ37923

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human/Mouse/Rat beta-Catenin Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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Targeting chemoresistant colorectal cancer via systemic administration of a BMP7 variant
Authors: V Veschi, LR Mangiapane, A Nicotra, S Di Franco, E Scavo, T Apuzzo, DS Sardina, M Fiori, A Benfante, ML Colorito, G Cocorullo, F Giuliante, C Cipolla, G Pistone, MR Bongiorno, A Rizzo, CM Tate, X Wu, S Rowlinson, LF Stancato, M Todaro, R De Maria, G Stassi
Oncogene, 2019-10-07;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: ICC
Dimethyloxalylglycine prevents bone loss in ovariectomized C57BL/6J mice through enhanced angiogenesis and osteogenesis.
Authors: Peng J, Lai Z, Fang Z, Xing S, Hui K, Hao C, Jin Q, Qi Z, Shen W, Dong Q, Bing Z, Fu D
PLoS ONE, 2014-11-13;9(11):e112744.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot
Insulin and LiCl synergistically rescue myogenic differentiation of FoxO1 over-expressed myoblasts.
Authors: Wu, Yi Ju, Fang, Yen Hsin, Chi, Hsiang C, Chang, Li Chiun, Chung, Shih Yin, Huang, Wei Chie, Wang, Xiao Wen, Lee, Kuan Wei, Chen, Shen Lia
PLoS ONE, 2014-02-13;9(2):e88450.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot
Chronic oxidative stress causes amplification and overexpression of ptprz1 protein tyrosine phosphatase to activate beta-catenin pathway.
Authors: Liu YT, Shang D, Akatsuka S, Ohara H, Dutta KK, Mizushima K, Naito Y, Yoshikawa T, Izumiya M, Abe K, Nakagama H, Noguchi N, Toyokuni S
Am. J. Pathol., 2007-11-30;171(6):1978-88.
Species: Rat
Sample Types: Whole Cells, Whole Tissue
Applications: ICC, IHC-P