Human/Mouse/Rat Calcineurin A Antibody

Catalog #: MAB2839
4 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB2839
MAB2839-SP

Detection of Human/Mouse/Rat Calcineurin A by Western Blot.

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Citations (4)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse/Rat Calcineurin A Antibody Summary

Species Reactivity

Human, Mouse, Rat

Specificity

Detects human, mouse, and rat Calcineurin A.

Source

Monoclonal Rat IgG₁ Clone # 302202

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Bovine brain Calcineurin holoenzyme (A and B subunits)

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human/Mouse/Rat Calcineurin A by Western Blot. Western blot shows lysates of HT-29 human colon adenocarcinoma cell line, TS1 mouse helper T cell line, and Nb2-11 rat lymphoma cell line. PVDF membrane was probed with 1 µg/mL Rat Anti-Human/Mouse/Rat Calcineurin A Monoclonal Antibody (Catalog # MAB2839) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). For additional reference, Recombinant Human Calcineurin (Catalog # 3160-CA) (1 ng) was included. A specific band for Calcineurin A was detected at approximately 59 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Calcineurin A

Calcineurin A, also known as PP2B and PPP3CA, is the 59 kDa catalytic subunit of the calcium/calmodulin-dependent protein phosphatase. When activated by calcium in the presence of the regulatory B subunit and calmodulin, Calcineurin A selectively removes phosphates from serine and threonine residues on target proteins. Although ubiquitously expressed, Calcineurin levels are highest in brain, where the phosphatase plays a role in the formation and retention of memories. Calcineurin activity is inhibited by the immunosuppressant drug cyclosporine A bound to cyclophilins.

Entrez Gene IDs

5530 (Human); 19055 (Mouse); 24674 (Rat)

Alternate Names

calcineurin A alpha; Calcineurin A; Calmodulin-dependent calcineurin A subunit alpha isoform; CALN; CALNA; CALNAcatalytic subunit, alpha isoform; CAM-PRP catalytic subunit; CNA; CNA1; CNA1CALNA1; EC 3.1.3.16; PP2B; PP3CA; PPP2B; PPP2BCCN1; PPP3CA; protein phosphatase 2B, catalytic subunit, alpha isoform; protein phosphatase 3 (formerly 2B), catalytic subunit, alpha isoform(calcineurin A alpha); protein phosphatase 3, catalytic subunit, alpha isozyme; serine/threonine-protein phosphatase 2B catalytic subunit alpha isoform

Product Datasheets

Product Datasheet

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Related Research Areas

Citations for Human/Mouse/Rat Calcineurin A Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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