Human/Mouse/Rat Calcineurin B Antibody Summary
Met1-Val170
Accession # P63098
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human/Mouse/Rat Calcineurin B by Western Blot. Western blot shows lysates of MCF-7 human breast cancer cell line, TS1 mouse helper T cell line, and PC-12 rat adrenal pheochromocytoma cell line. PVDF membrane was probed with 0.5 µg/mL of Human/Mouse/Rat Calcineurin B Monoclonal Antibody (Catalog # MAB1348) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Calcineurin B at approximately 18 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Calcineurin B
Background
Calcineurin, also called Protein Phosphatase 2B,
PP2B, PPP2B, Protein Phosphatase 3, and PPP3, is
an enzyme that dephosphorylates serine and
threonine residues in proteins. It is a heterodimer of
a 59,000 dalton catalytic A subunit and a
1,900 dalton regulatory B subunit that is activated
by the binding of calcium ions and calmodulin (1).
Calcineurin is expressed in many tissues, but its
levels are highest in the brain, where it may play a
role in learning and memory (2). It has many
substrates, including NFAT, a transcription factor
that is activated by dephosphorylation (3).
Complexes of the immunosuppressants cyclosporin
and FK506 with immunophilin proteins such as
cyclophilin and FKBP12 are potent and specific
inhibitors of Calcineurin activity (4). Alterations in
Calcineurin activity are suspected to play a role in
cardiac hypertrophy (5) and graft versus host
disease in organ transplantation (6).
References:
1. Stemmer, P.M and C.B. Klee (1994) Biochemistry 33:6859.
2. Zeng H. et al. (2001) Cell 107:617.
3. Okamura, H. et al. (2000) Molecular Cell 6:539.
4. Liu, J. et al. (1992) Biochemistry 31:3896.
5. Molkentin, J.D. (2000) Circulation Research 87:731.
6. Sanquer, S.A. et al. (2004) Transplantation 77:854.
Product Datasheets
Citations for Human/Mouse/Rat Calcineurin B Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 2
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Ppt1-deficiency dysregulates lysosomal Ca++ -homeostasis contributing to pathogenesis in a mouse model of CLN1 disease
Authors: Mondal A, Appu AP, Sadhukhan T et al.
Journal of inherited metabolic disease
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Calcineurin B1 subunit in human peripheral blood mononuclear cells and its role in idiopathic membranous nephropathy
Authors: Ying Zhu, Min Zhang, Fan Wang, Jing Sun, Jianda Lu, Ruiying Chen et al.
Medicine (Baltimore)
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