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Human/Mouse/Rat CDC14 Antibody

Catalog #: MAB4457
1 Citation Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB4457
MAB4457-SP
Detection of Human and Mouse CDC14 by Western Blot.
2 Images
Product Details
Citations (1)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse/Rat CDC14 Antibody Summary

Species Reactivity
Human, Mouse, Rat
Specificity
Detects endogenous human, mouse and rat CDC14A in Western blots. In Western blots, this antibody does not cross‑react with recombinant human PTEN. Reactivity with CDC14B is not known.
Source
Monoclonal Mouse IgG1 Clone # 472508
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human CDC14A
Met15-Gly366
Accession # Q9UNH5
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human and Mouse CDC14 antibody by Western Blot. View Larger

Detection of Human and Mouse CDC14 by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, Raji human Burkitt's lymphoma cell line, and C2C12 mouse myoblast cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat CDC14 Monoclonal Antibody (Catalog # MAB4457) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for CDC14 at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Western Blot Detection of Human, Mouse, and Rat CDC14 antibody by Western Blot. View Larger

Detection of Human, Mouse, and Rat CDC14 by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, C2C12 mouse myoblast cell line, H4-II-E-C3 rat hepatoma cell line, L6 rat myoblast cell line, and Rat-2 rat embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse/Rat CDC14 Monoclonal Antibody (Catalog # MAB4457) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). Specific bands were detected for CDC14 at approximately 50-75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CDC14

Cell Division Cycle 14 (CDC14) is a dual tyrosine and serine/threonine phosphatase. There are two CDC14 proteins, CDC14A with a molecular weight of 70 kDa, and CDC14B with a molecular weight of 53 kDa. CDC14A is primarily associated with the nucleolus, bound to Cfi1/Net1 during cell cycle interphase. During anaphase and telophase, CDC14 redistributes to the nucleoplasm and cytoplasm, where it plays a role in segregating and separating sister chromatids. Mutations that alter CDC14 function cause uneven distribution of chromosomes in dividing cells.

Long Name
Cell Division Cycle 14
Entrez Gene IDs
8556 (Human); 229776 (Mouse); 310806 (Rat)
Alternate Names
CDC14; cdc14;CDC14A;DFNB105;DFNB32;DFNB35;Dual specificity protein phosphatase CDC14A;hCDC14; CDC14A

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Citation for Human/Mouse/Rat CDC14 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. The non-classical MAP kinase ERK3 controls T cell activation.
    Authors: Marquis M, Boulet S, Mathien S, Rousseau J, Thebault P, Daudelin J, Rooney J, Turgeon B, Beauchamp C, Meloche S, Labrecque N
    PLoS ONE, 2014-01-27;9(1):e86681.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot

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