Human/Mouse/Rat CDC25A Antibody Summary
Glu2-Leu523
Accession # AAA58415
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human/Mouse/Rat CDC25A by Western Blot. Western blot shows lysates of MCF-7 human breast cancer cell line, CTLL-2 mouse cytotoxic T cell line, and Nb2-11 rat lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat CDC25A Monoclonal Antibody (Catalog # MAB1648) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Specific bands were detected for CDC25A at approximately 55-60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CDC25A
Cell Division Cycle 25A (CDC25A) phosphatase removes inorganic phosphate groups covalently attached to tyrosine, serine and threonine residues in proteins (1). Overexpression of CDC25A in small mammary carcinomas has been associated with a poor patient survival prognosis (2). Levels of CDC25A activity are highest in S phase of the cell cycle, where it is suspected to be involved in “checkpoint” control of cell cycle progression (3). Induction of DNA damage with ultraviolet or ionizing radiation causes an increase in CDC25A ubiquinylation and proteosomal degradation, leading to cell cycle block between G1 and S phases (4). One of its major substrates, the kinase CDC2, is activated by dephosphorylation (5). Recombinant CDC25A is truncated to remove the N-terminal regulatory domains and is fully active.
- Draetta, G. and J. Eckstein (1997) Biochim. Biophys. Acta. 1332:M53.
- Cangi, M. et al. (2000) J. Clin. Invest. 106:753.
- Hoffmann, I. et al. (1995) EMBO J. 13:4302.
- Sagata, N. (2002) Science 298:1905.
- Gautier, J. et al. (1991) Cell 67:197.
Product Datasheets
Citations for Human/Mouse/Rat CDC25A Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 2
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Chemotherapy-induced differential cell cycle arrest in B cell lymphomas affects their sensitivity to Wee1 inhibition
Authors: X Wang, Z Chen, AK Mishra, A Silva, W Ren, Z Pan, JH Wang
Haematologica, 2017-12-07;0(0):.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
Aurora kinase A is a target of Wnt/beta-catenin involved in multiple myeloma disease progression.
Authors: Dutta-Simmons J, Zhang Y, Gorgun G, Gatt M, Mani M, Hideshima T, Takada K, Carlson NE, Carrasco DE, Tai YT, Raje N, Letai AG, Anderson KC, Carrasco DR
Blood, 2009-08-03;114(13):2699-708.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
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