Human/Mouse/Rat Contactin-3 Antibody Summary
Glu20-Thr1003
Accession # Q07409
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human/Mouse/Rat Contactin‑3 by Western Blot. Western blot shows lysates of mouse, human, and rat brain tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat Contactin-3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5539) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Contactin-3 at approximately 150 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
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Contactin‑3 in bEnd.3 Mouse Cell Line. Contactin-3 was detected in immersion fixed bEnd.3 mouse endothelioma cell line using Goat Anti-Human/Mouse/Rat Contactin-3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5539) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the Northern-Lights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Contactin-3
Contactin-3 (also BIG-1 in rat and PANG in mouse) is a 150 kDa member of the TAG/F3 subgroup, contactin family, Ig superfamily hierarchy. It is a GPI linked glycoprotein that is expressed on cerebellar Purkinje cells, amygdaloid and thalamic neurons and olfactory granule cells. Its exact function is unclear, but it likely mediates cell-cell interaction and may promote neurite outgrowth. Human Contactin-3 preproprecursor is 1028 amino acids (aa) in length. It contains a 19 aa signal sequence, a 983 aa mature region and a 26 aa cleavable C-terminal prosegment. The mature region shows six C2- type IG like domains (aa 26-593), followed by four fibronectin type III modules (aa 598-992). There is one potential alternate start site at Met128. Over aa 26-992, human Contactin-3 shares 92% aa identity with mouse Contactin‑3.
Product Datasheets
Citation for Human/Mouse/Rat Contactin-3 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
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Down-regulation of the brain-specific cell-adhesion molecule contactin-3 in tuberous sclerosis complex during the early postnatal period
Authors: A Korotkov, MJ Luinenburg, A Romagnolo, TS Zimmer, J van Schepp, A Bongaarts, DWM Broekaart, JJ Anink, C Mijnsberge, FE Jansen, W van Hecke, WG Spliet, PC van Rijen, M Feucht, JA Hainfellne, P Krsek, J Zamecnik, PB Crino, K Kotulska, L Lagae, AC Jansen, DJ Kwiatkowsk, S Jozwiak, P Curatolo, A Mühlebner, EA van Vliet, JD Mills, E Aronica
Journal of neurodevelopmental disorders, 2022-01-15;14(1):8.
Species: Human
Sample Types: Tissue Homogenates
Applications: Western Blot
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