Human/Mouse/Rat DDX1 Antibody

Catalog #: MAB9615 Datasheet / COA / SDS

Recombinant Monoclonal Antibody

Catalog #	Availability	Size / Price	Qty
MAB9615-100
MAB9615-SP

Detection of Human, Mouse, and Rat DDX1 by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse/Rat DDX1 Antibody Summary

Species Reactivity

Human, Mouse, Rat

Specificity

Detects human, mouse, and rat DDX1 in Western blots.

Source

Monoclonal Rabbit IgG Clone # 2220A

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.5 µg/mL

See below

Immunohistochemistry

3-25 µg/mL

See below

Immunocytochemistry

1-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human, Mouse, and Rat DDX1 by Western Blot. Western blot shows lysates of Y-79 human retinoblastoma cell line, A431 human epithelial carcinoma cell line, NIH-3T3 mouse embryonic fibroblast cell line, Neuro-2A mouse neuroblastoma cell line, and Rat-2 rat embryonic fibroblast cell line. PVDF membrane was probed with 0.5 µg/mL of Rabbit Anti-Human/Mouse/Rat DDX1 Monoclonal Antibody (Catalog # MAB9615) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for DDX1 at approximately 85 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry

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DDX1 in HeLa Human Cell Line. DDX1 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Rabbit Anti-Human/Mouse/Rat DDX1 Monoclonal Antibody (Catalog # MAB9615) at 1 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunohistochemistry

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DDX1 in Human Breast Cancer Tissue. DDX1 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Rabbit Anti-Human/Mouse/Rat DDX1 Monoclonal Antibody (Catalog # MAB9615) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell nuclei. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.

Background: DDX1

DEAD (Asp-Glu-Ala-Asp) box helicase 1 (DDX1) is a putative RNA helicase gene. RNA helicases are involved in various cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. It has been proposed that DDX1 plays a role in RNA clearance at DNA double-strand breaks. It has also been reported as a coactivator of the NF-kappa-B promoter. DDX1 also acts as a positive transcriptional regulator of cyclin CCND2 expression. Human DDX1 has 97% homology to mouse and rat DDX1. Expression in embryos and testis suggest that DDX1 mediates cell growth and development. DDX1 has been proposed as a diagnostic marker for early recurrence in primary breast cancer.

Long Name

DEAD-Box Helicase 1

Entrez Gene IDs

1653 (Human); 104721 (Mouse); 84474 (Rat)

Alternate Names

ATP-dependent RNA helicase DDX1; DBP-RB; DBP-RBDEAD box polypeptide 1; DDX1; DEAD (Asp-Glu-Ala-Asp) box polypeptide 1; DEAD box protein 1; DEAD box protein retinoblastoma; DEAD Box-1; DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 1; DEAD/H-Box Helicase 1; EC 3.6.1; EC 3.6.1.10; EC 3.6.4.13; UKVH5d