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Human/Mouse/Rat ERK1/ERK2 Antibody

Catalog #: AF1576
17 Citations 2 Reviews Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF1576
AF1576-SP
Detection of Human/Mouse/Rat ERK1/ERK2 by Western Blot.
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Product Details
Citations (17)
FAQs
Supplemental Products
Reviews (2)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse/Rat ERK1/ERK2 Antibody Summary

Species Reactivity
Human, Mouse, Rat
Specificity
Detects human, mouse and rat ERK1/ERK2.
Source
Polyclonal Rabbit IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human ERK1/ERK2
XM_055766 and NM_138957, respectively
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated
Purity
Immunogen affinity purified

Applications

Recommended Concentration
Sample
Western Blot
0.2 µg/mL
See below
Simple Western
10 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human/Mouse/Rat ERK1/ERK2 antibody by Western Blot. View Larger

Detection of Human/Mouse/Rat ERK1/ERK2 by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line, C2C12 mouse myoblast cell line myoblast cell line, and C6 rat glioma cell line. PVDF membrane was probed with 0.2 µg/mL Rabbit Anti-Human/Mouse/Rat ERK1/ERK2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1576) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). For additional reference, Recombinant Human Active ERK1 (Catalog # 1879-KS) and Recombinant Human Active ERK2 (Catalog # 1230-KS) (2 ng/lane) were included. A specific band for ERK1/ERK2 was detected at approximately 44 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western Detection of Mouse ERK1/ERK2 antibody by Simple Western<SUP>TM</SUP>. View Larger

Detection of Mouse ERK1/ERK2 by Simple WesternTM. Simple Western lane view shows lysates of C2C12 mouse myoblast cell line, loaded at 0.2 mg/mL. A specific band was detected for ERK1/ERK2 at approximately 44 kDa (as indicated) using 10 µg/mL of Rabbit Anti-Human/Mouse/Rat ERK1/ERK2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1576). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ERK1/ERK2

ERK1 and ERK2 (also known as MAPK3 and MAPK1) are 44 and 42 kDa Ser/Thr kinases, respectively. They are part of the Ras-Raf-ERK signal transduction cascade often found downstream of growth factor receptor activation. ERK1 and ERK2 were initially isolated and cloned as kinases activated in response to insulin and NGF. They are expressed in most, if not all, mammalian tissues. Dual threonine and tyrosine phosphorylation activate both ERKs, at Thr202/Tyr204 for human ERK1 and Thr185/Tyr187 for human ERK2.

ERK5, also known as Big Mitogen-activated Protein Kinase 1 (BMK1) and MAPK7, is activated by several mechanisms, including receptor tyrosine kinases, G protein-coupled receptors, and osmotic stress. Like ERK1 and ERK2, ERK5 contains the conserved Thr-Glu-Tyr activation motif in its activation loop. Unlike these ERKs, however, ERK5 contains a unique C-terminal domain that regulates its activation and nuclear translocation.

Long Name
Extracellular Signal-regulated Kinase 1/2
Alternate Names
ERK1/ERK2

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Citations for Human/Mouse/Rat ERK1/ERK2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

17 Citations: Showing 1 - 10
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  1. Dose-Dependent Biphasic Action of Quetiapine on AMPK Signalling via 5-HT7 Receptor: Exploring Pathophysiology of Clinical and Adverse Effects of Quetiapine
    Authors: M Okada, K Fukuyama, E Motomura
    International Journal of Molecular Sciences, 2022-08-14;23(16):.
    Species: Rat
    Sample Types: Cell Lysates
    Applications: Simple Western
  2. Brexpiprazole Reduces 5-HT7 Receptor Function on Astroglial Transmission Systems
    Authors: K Fukuyama, E Motomura, M Okada
    International Journal of Molecular Sciences, 2022-06-12;23(12):.
    Species: Rat
    Sample Types: Cell Lysates
    Applications: Western Blot
  3. Brivaracetam and Levetiracetam Suppress Astroglial L-Glutamate Release through Hemichannel via Inhibition of Synaptic Vesicle Protein
    Authors: K Fukuyama, M Okada
    International Journal of Molecular Sciences, 2022-04-19;23(9):.
    Species: Rat
    Sample Types: Cell Lysates
    Applications: Capillary Immunoblot
  4. Distinct Effects of Escitalopram and Vortioxetine on Astroglial L-Glutamate Release Associated with Connexin43
    Authors: T Shiroyama, K Fukuyama, M Okada
    International Journal of Molecular Sciences, 2021-09-16;22(18):.
    Species: Rat
    Sample Types: Cell Lysates
    Applications: Capillary Immunoblot
  5. Autocrine TGF&beta1 Opposes Exogenous TGF&beta1-Induced Cell Migration and Growth Arrest through Sustainment of a Feed-Forward Loop Involving MEK-ERK Signaling
    Authors: H Ungefroren, J Christl, C Eiden, UF Wellner, H Lehnert, JU Marquardt
    Cancers, 2021-03-17;13(6):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  6. IL-24 deficiency protects mice against bleomycin-induced pulmonary fibrosis by repressing IL-4-induced M2 program in macrophages
    Authors: LZ Rao, Y Wang, L Zhang, G Wu, L Zhang, FX Wang, LM Chen, F Sun, S Jia, S Zhang, Q Yu, JH Wei, HR Lei, T Yuan, J Li, X Huang, B Cheng, J Zhao, Y Xu, BW Mo, CY Wang, H Zhang
    Cell Death Differ, 2020-11-03;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  7. DRD3 (dopamine receptor D3) but not DRD2 activates autophagy through MTORC1 inhibition preserving protein synthesis
    Authors: P Barroso-Ch, D Luis-Ravel, F Fumagallo-, J Castro-Her, J Salas-Hern, J Rodriguez-, A Febles-Cas, I Cruz-Muros, D Afonso-Ora, P Abreu-Gonz, R Moratalla, MJ Millan, T Gonzalez-H
    Autophagy, 2019-10-02;0(0):.
    Species: Human, Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  8. Characterization of TRKA signaling in acute myeloid leukemia
    Authors: SM Herbrich, S Kannan, RM Nolo, M Hornbaker, J Chandra, PA Zweidler-M
    Oncotarget, 2018-07-10;9(53):30092-30105.
    Species: Human
    Sample Types: Whole Cells
    Applications: Western Blot
  9. Effect of electrical stimulation on neural regeneration via the p38-RhoA and ERK1/2-Bcl-2 pathways in spinal cord-injured rats
    Authors: MC Joo, CH Jang, JT Park, SW Choi, S Ro, MS Kim, MY Lee
    Neural Regen Res, 2018-02-01;13(2):340-346.
    Species: Rat
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  10. The Role of PAR2 in TGF-?1-Induced ERK Activation and Cell Motility
    Authors: H Ungefroren, D Witte, C Fiedler, T Gädeken, R Kaufmann, H Lehnert, F Gieseler, BH Rauch
    Int J Mol Sci, 2017-12-20;18(12):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  11. Negative regulation of TGF-?1-induced MKK6-p38 and MEK-ERK signalling and epithelial-mesenchymal transition by Rac1b
    Authors: D Witte, H Otterbein, M Förster, K Giehl, R Zeiser, H Lehnert, H Ungefroren
    Sci Rep, 2017-12-11;7(1):17313.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  12. Obesity Increases Mitogen-Activated Protein Kinase Phosphatase-3 Levels in the Hypothalamus of Mice
    Authors: BA Rodrigues, VR Muñoz, GK Kuga, RC Gaspar, SCBR Nakandakar, BM Crisol, JD Botezelli, LSS Pauli, ASR da Silva, LP de Moura, DE Cintra, ER Ropelle, JR Pauli
    Front Cell Neurosci, 2017-10-09;11(0):313.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  13. TNF-alpha induces CXCL1 chemokine expression and release in human vascular endothelial cells in vitro via two distinct signaling pathways.
    Authors: Lo H, Lai T, Li C, Wu W
    Acta Pharmacol Sin, 2014-02-03;35(3):339-50.
    Species: Human
    Sample Types: N/A
  14. A naturally occurring carotenoid, lutein, reduces PDGF and H2O2 signaling and compromised migration in cultured vascular smooth muscle cells.
    Authors: Lo HM, Tsai YJ, Du WY, Tsou CJ, Wu WB
    J. Biomed. Sci., 2012-02-08;19(0):18.
    Species: Rat
    Sample Types: Cell Lysates
    Applications: Western Blot
  15. The EGFR ligands amphiregulin and heparin-binding egf-like growth factor promote peritoneal carcinomatosis in CXCR4-expressing gastric cancer.
    Authors: Yasumoto K, Yamada T, Kawashima A, Wang W, Li Q, Donev IS, Tacheuchi S, Mouri H, Yamashita K, Ohtsubo K, Yano S
    Clin. Cancer Res., 2011-04-11;17(11):3619-30.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  16. Transient PI3K Inhibition Induces Apoptosis and Overcomes HGF-Mediated Resistance to EGFR-TKIs in EGFR Mutant Lung Cancer.
    Authors: Donev IS, Wang W, Yamada T, Li Q, Takeuchi S, Matsumoto K, Yamori T, Nishioka Y, Sone S, Yano S
    Clin. Cancer Res., 2011-01-10;17(8):2260-9.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  17. CB(2) cannabinoid receptor activation is cardioprotective in a mouse model of ischemia/reperfusion.
    Authors: Montecucco F, Lenglet S, Braunersreuther V, Burger F, Pelli G, Bertolotto M, Mach F, Steffens S
    J. Mol. Cell. Cardiol., 2009-01-07;46(5):612-20.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot

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Reviews for Human/Mouse/Rat ERK1/ERK2 Antibody

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Human/Mouse/Rat ERK1/ERK2 Antibody
By Anonymous on 11/16/2019
Application: WB Sample Tested: Hematological Cancer cell lines Species: Human

20 ug protein per sample
1:1000 dilution for Antibody.

Western Blot Human/Mouse/Rat ERK1/ERK2 Antibody AF1576
Human/Mouse/Rat ERK1/ERK2 Antibody
By Anonymous on 07/17/2018
Application: WB Sample Tested: HEK293 human embryonic kidney cell line Species: Human
Western Blot Human/Mouse/Rat ERK1/ERK2 Antibody AF1576