Human/Mouse/Rat Fc mu R/FAIM3 Antibody

Catalog #: MAB9494 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB9494-100
MAB9494-SP

Detection of Human, Mouse, and Rat Fc mu R/FAIM3 by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse/Rat Fc mu R/FAIM3 Antibody Summary

Species Reactivity

Human, Mouse, Rat

Specificity

Detects human Fc mu R/FAIM3 in direct ELISAs. Detects human, mouse, and rat Fc mu R/FAIM3 in Western blots.

Source

Monoclonal Mouse IgG_2B Clone # 992338

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Human embryonic kidney cell line HEK293-derived recombinant human Fc mu R/FAIM3
Arg18-Gly251
Accession # O60667

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

2 µg/mL

See below

Flow Cytometry

0.25 µg/10⁶ cells

See below

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human, Mouse, and Rat Fc mu R/FAIM3 by Western Blot. Western blot shows lysates of K562 human chronic myelogenous leukemia cell line, A20 mouse B cell lymphoma cell line, and Nb2-11 rat lymphoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human/Mouse/Rat Fc mu R/FAIM3 Monoclonal Antibody (Catalog # MAB9494) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Fc mu R/FAIM3 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Flow Cytometry

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Detection of FAIM3 in HEK293 Human Cell Line Transfected with Human FAIM3 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with (A) human FAIM3 or (B) irrelevant transfectants and eGFP was stained with Mouse Anti-Human/Mouse/Rat FAIM3 Monoclonal Antibody (Catalog # MAB9494) followed by APC-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB0041). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Fc mu R/FAIM3

The human FAIM3 gene (also known as FCMR or TOSO), is a transmembrane sialoglycoprotein expressed mainly by lymphocytes. FAIM3 is a type I membrane protein with an intracellular C-terminal domain and an extracellular N-terminal domain (1). The extracellular domain has homology to the immunoglobulin variable region domains (1) and FAIM3 is identified as an Fc receptor for IgM (2, 3). The amino acid sequence of human FAIM3 is 58% and 55% identical to that of mouse and rat FAIM3, respectively. FAIM3 was shown to be over-expressed in chronic lymphocytic leukemia (CLL) (4) and associated with disease progression (5, 6). FAIM3 has also been linked to the homeostasis and activation of the innate immune system (7). Interestingly, there is growing evidence that neurodegenerative diseases are associated with the activation of the immune surveillance system. This system is responsible for controlling danger signals and responding accordingly to the magnitude and duration of the threat (8, 9).

References

Song, Y. et al (2005) J. Biol. Chem. 280(10):9618.
Shima H et al. (2010) Int. Immunol. 22:149.
Kubagawa H et al. (2009) J. Exp. Med. 206:2779.
Proto-Siqueira R et al. (2008) Blood, 112:394.
Pallasch, C et al. (2008) Blood, 112:4213.
Pallasch, C P et al (2009) Leukemia & lymphoma, 50:498.
Sigruener, A et al (2007) Biochemical and biophysical research communications, 359:723.
Richards R I et al (2016) Front. Neurosci. 10:193.
Planells-Ferrer L et al (2016) J Neurochem. 139:11.

Long Name

Fc mu Receptor

Entrez Gene IDs

9214 (Human); 69169 (Mouse); 548326 (Rat)

Alternate Names

FAIM3; Fas apoptotic inhibitory molecule 3; Fc mu R; Fc mu receptor; FCMR; immunoglobulin mu Fc receptor; Regulator of Fas-induced apoptosis Toso; Toso; TOSOIgM Fc receptor