Human/Mouse/Rat FKBP38 Antibody Summary
Met1-Gly326
Accession # AAH03739
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human/Mouse/Rat FKBP38 by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, MDA-MB-468 human breast cancer cell line, A549 human lung carcinoma cell line, C2C12 mouse myoblast cell line, and L6 rat myoblast cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human/Mouse/Rat FKBP38 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3580) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for FKBP38 at approximately 60 - 64 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
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FKBP38 in Human Intestine. FKBP38 was detected in immersion fixed paraffin-embedded sections of human intestine using 15 µg/mL Goat Anti-Human/Mouse/Rat FKBP38 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3580) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to the nucleus of epithelial cells in intestinal glands. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections. This application has not been tested in rat or mouse tissue.
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Detection of Human FKBP38 by Simple WesternTM. Simple Western lane view shows lysates of HepG2 human hepatocellular carcinoma cell line and MDA-MB-468 human breast cancer cell line, loaded at 0.2 mg/mL. A specific band was detected for FKBP38 at approximately 58 kDa (as indicated) using 5 µg/mL of Goat Anti-Human/Mouse/Rat FKBP38 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3580) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: FKBP38
FK506 Binding Proteins (FKBPs) are intracellular receptors for the immuno-suppressive drug FK506. The FKBP/FK506 complex exerts its immunosuppressive effects by inhibiting calcineurin, a calcium- and calmodulin-dependent serine/threonine phosphatase that functions as a critical signaling molecule during T cell activation. FKBP38, also known as FKBP8, is a 355 amino acid (aa) protein with a calculated molecular weight of 38.7 kDa and an apparent molecular mass of ~60-64 kDa in SDS-PAGE. FKBP38 binds to and inhibits calcineurin even in the absence of FK506, indicating that FKBP38 is a constitutively active inhibitor of calcineurin. Additionally, FKBP38 immunoprecipitates with Bcl-2 and Bcl-xL, suggesting that FKBP38 may regulate apoptosis by anchoring Bcl-2 and Bcl-xL to mitochondrial membranes. Mouse FKBP38 shares 94% and 87% sequence identity with human and rat FKBP38, respectively.
Product Datasheets
Citation for Human/Mouse/Rat FKBP38 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
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Hepatitis C virus NS5A activates the mammalian target of rapamycin (mTOR) pathway, contributing to cell survival by disrupting the interaction between FK506-binding protein 38 (FKBP38) and mTOR.
Authors: Peng L, Liang D, Tong W
J. Biol. Chem., 2010-05-03;285(27):20870-81.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
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