Human/Mouse/Rat FoxP3 Alexa Fluor® 647-conjugated Antibody
Human/Mouse/Rat FoxP3 Alexa Fluor® 647-conjugated Antibody Summary
Gln105-Lys200
Accession # Q9BZS1
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of FoxP3 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human/Mouse/Rat FoxP3 Alexa Fluor® 647-conjugated Monoclonal Antibody (Catalog # IC8970R) and Mouse Anti-Human CD4 Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB3791F). Quadrant markers were set based on control antibody staining (Catalog # IC002R). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules.
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Detection of FoxP3 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human/Mouse/Rat FoxP3 Alexa Fluor® 647-conjugated Monoclonal Antibody (Catalog # IC8970R) and Mouse Anti-Human CD25/IL-2 R alpha PE-conjugated Monoclonal Antibody (Catalog # FAB1020P). Quadrant markers were set based on control antibody staining (Catalog # IC002R). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules.
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Detection of FoxP3 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Mouse Anti-Human/Mouse/Rat FoxP3 Alexa Fluor® 647-conjugated Monoclonal Antibody (Catalog # IC8970R) and Rat Anti-Mouse CD4 Alexa Fluor® 488-conjugated Monoclonal Antibody (Catalog # FAB554G). Quadrant markers were set based on control antibody staining (Catalog # IC002R). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules.
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Detection of FoxP3 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Mouse Anti-Human/Mouse/Rat FoxP3 Alexa Fluor® 647-conjugated Monoclonal Antibody (Catalog # IC8970R) and Rat Anti-Mouse CD25/IL-2 R alpha PE-conjugated Monoclonal Antibody (Catalog # FAB2438P). Quadrant markers were set based on control antibody staining (Catalog # IC002R). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules.
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Detection of FoxP3 in Rat Splenocytes by Flow Cytometry. Rat splenocytes were stained with Mouse Anti-Human/Mouse/Rat FoxP3 Alexa Fluor® 647-conjugated Monoclonal Antibody (Catalog # IC8970R) and Anti-Rat CD4 APC-conjugated Antibody. Quadrant markers were set based on control antibody staining (Catalog # IC002R). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules.
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Preparation and Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: FoxP3
Human FoxP3 is a 431 amino acid (aa), 47 kDa member of the P subclass of the FOX (forkhead box) family of transcription factors. It contains a Leu-rich repeat, a C2H2 zinc finger region, and a C-terminal FKH (fork head), DNA-binding domain. Three alternative splicing events have been described in human. The first shows a deletion of aa 72-106, a second exhibits a deletion of aa 246-272, and a third records a 60 aa insertion after Lys382. These events are likely not to be mutually exclusive. The first isoform (plus the full-length form) occurs in CD4+CD25+ T cells, while the less frequent second isoform appears in CD8+ Tregs. The mouse FoxP3 gene does not appear to be spliced. In addition, FoxP3 is found only in Tregs in mouse, while human FoxP3 appears in both Tregs and CD4+ and CD8+ conventional T cells.
Both mouse and human FoxP3 undergo proteolytic processing, after Arg51 and Arg417 in human, and at equivalent sites in mouse. Cleavage at the N-terminus generate a 41 kDa isoform, whereas C-terminal cleavage generates an isoform indistinguishable from the full-length isoform (47 kDa) in SDS-PAGE.
FoxP3 can act as a transcriptional repressor (for IL-2) via an interaction with HDAC7. By contrast, it can also act as a transcriptional activator (for GITRL and
CTLA-4) through an interaction with RUNX7. Notably, for both human and mouse, full-length 47 kDa FoxP3 will induce high levels of CTLA-4 on expressing cells. However, when the C-terminally truncated isoform (at Arg417) is considered, IL-10 promoting activity is enhanced in mouse, while suppressive activity is lost in human. Over aa 105-200, human FoxP3 shares 83% and 84% aa sequence identity with rat and mouse FoxP3, respectively.
Product Datasheets
Product Specific Notices
This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.
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