Human/Mouse/Rat FRS2 Antibody

Catalog # Availability Size / Price Qty
MAB4069
MAB4069-SP
Detection of Human/Mouse/Rat FRS2 by Western Blot.
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Product Details
Citations (6)
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Human/Mouse/Rat FRS2 Antibody Summary

Species Reactivity
Human, Mouse, Rat
Specificity
Detects human, mouse, and rat FRS2 in Western blots.
Source
Monoclonal Mouse IgG2B Clone # 462910
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human FRS2
Asn121-Asn449
Accession # Q8WU20
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS and NaCl with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Purity
Protein A or G purified from hybridoma culture supernatant

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human/Mouse/Rat FRS2 antibody by Western Blot. View Larger

Detection of Human/Mouse/Rat FRS2 by Western Blot. Western blot shows lysates of MCF-7 human breast cancer cell line, C2C12 mouse myoblast cell line, and PC-12 rat adrenal pheochromocytoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat FRS2 Monoclonal Antibody (Catalog # MAB4069) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for FRS2 at approximately 85 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FRS2

FRS2 (FGF R substrate 2; also known as SNT and FRS2 alpha ) is a 70‑90 kDa member of the FRS family of lipid-anchored docking proteins. It is an intermediary between FGF R and other RTK receptors and their Ras/MAPK signaling cascades. FRS2 contains a membrane-anchoring myristoylation signal (aa 1‑6), a PTB domain (aa 13‑115) that interacts with FGF and NGF receptors, and a C-terminal Tyr-rich region (aa 196‑471) that serves as a docking site for GRB‑2 and SHP‑2.

Long Name
Fibroblast Growth Factor Receptor Substrate 2
Entrez Gene IDs
10818 (Human); 327826 (Mouse)
Alternate Names
FGFR signalling adaptor; FGFR-signaling adaptor SNT; fibroblast growth factor receptor substrate 2; FRS2; FRS2A; FRS2alpha; SNT; SNT1; SNT-1FGFR substrate 2; Suc1-associated neurotrophic factor target 1

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Citations for Human/Mouse/Rat FRS2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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  1. Fibroblast growth factor signalling influences homologous recombination-mediated DNA damage repair to promote drug resistance in ovarian cancer
    Authors: HA Nicholson, L Sawers, RG Clarke, KJ Hiom, MJ Ferguson, G Smith
    British Journal of Cancer, 2022-07-01;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. Whole-genome sequencing identifies ADGRG6 enhancer mutations and FRS2 duplications as angiogenesis-related drivers in bladder cancer
    Authors: S Wu, T Ou, N Xing, J Lu, S Wan, C Wang, X Zhang, F Yang, Y Huang, Z Cai
    Nat Commun, 2019-02-12;10(1):720.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  3. Bradykinin B2 Receptor Contributes to Inflammatory Responses in Human Endothelial Cells by the Transactivation of the Fibroblast Growth Factor Receptor FGFR-1
    Authors: E Terzuoli, F Corti, G Nannelli, A Giachetti, S Donnini, M Ziche
    Int J Mol Sci, 2018-09-06;19(9):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  4. Gambogenic acid inhibits fibroblast growth factor receptor signaling pathway in erlotinib-resistant non-small-cell lung cancer and suppresses patient-derived xenograft growth
    Authors: L Xu, X Meng, N Xu, W Fu, H Tan, L Zhang, Q Zhou, J Qian, S Tu, X Li, Y Lao, H Xu
    Cell Death Dis, 2018-02-15;9(3):262.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  5. Renal expression of FGF23 and peripheral resistance to elevated FGF23 in rodent models of polycystic kidney disease.
    Authors: Spichtig D, Zhang H, Mohebbi N, Pavik I, Petzold K, Stange G, Saleh L, Edenhofer I, Segerer S, Biber J, Jaeger P, Serra A, Wagner C
    Kidney Int, 2014-01-08;85(6):1340-50.
    Species: Rat
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  6. Phosphorylation and lipid raft association of fibroblast growth factor receptor-2 in oligodendrocytes.
    Authors: Bryant MR, Marta CB, Kim FS, Bansal R
    Glia, 2009-07-01;57(9):935-46.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot

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