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Human/Mouse/Rat GAD1/GAD67 Antibody

Catalog #: AF2086
13 Citations 1 Review Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF2086
AF2086-SP
Detection of Human, Mouse, and Rat GAD1/GAD67 by Western Blot.
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Product Details
Citations (13)
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Supplemental Products
Reviews (1)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse/Rat GAD1/GAD67 Antibody Summary

Species Reactivity
Human, Mouse, Rat
Specificity
Detects human GAD1/GAD67 in direct ELISAs and detects human, mouse, and rat GAD1/GAD67 in Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human GAD1/GAD67
Ala2-Asn97
Accession # Q99259
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Simple Western
1 µg/mL
See below
Immunohistochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human, Mouse, and Rat GAD1/GAD67 antibody by Western Blot. View Larger

Detection of Human, Mouse, and Rat GAD1/GAD67 by Western Blot. Western blot shows lysates of human brain (hypothalamus) tissue, mouse brain (total) tissue, and rat brain (total) tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat GAD1/GAD67 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2086) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody HAF017). A specific band was detected for GAD1/GAD67 at approximately 67 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry GAD1/GAD67 antibody in Human Spinal Cord by Immunohistochemistry (IHC-P). View Larger

GAD1/GAD67 in Human Spinal Cord. GAD1/GAD67 was detected in immersion fixed paraffin-embedded sections of human spinal cord using 15 µg/mL Goat Anti-Human/Mouse/Rat GAD1/GAD67 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2086) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Simple Western Detection of Mouse GAD1/GAD67 antibody by Simple Western<sup>TM</sup>. View Larger

Detection of Mouse GAD1/GAD67 by Simple WesternTM. Simple Western lane view shows lysates of mouse brain tissue, loaded at 0.2 mg/mL. A specific band was detected for GAD1/GAD67 at approximately 65 kDa (as indicated) using 1 µg/mL of Goat Anti-Human/Mouse/Rat GAD1/GAD67 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2086) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GAD1/GAD67

GAD1, also named 67 kDa or brain GAD, is an enzyme that catalyzes the formation of the inhibitory neurotransmitter gamma -amino butyric acid (GABA) from glutamate. GAD1 is also expressed in multiple non-neuronal tissues during development.

Long Name
Glutamate Decarboxylase 1 (Brain, 67kDa)
Entrez Gene IDs
2571 (Human)
Alternate Names
67kD); EC 4.1.1; FLJ45882; GAD1; GAD25; GAD67; glutamate decarboxylase 1 (brain, 67kDa); Glutamate decarboxylase 67 kDa isoform

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Citations for Human/Mouse/Rat GAD1/GAD67 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

13 Citations: Showing 1 - 10
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  1. Altered Parvalbumin Basket Cell Terminals in the Cortical Visuospatial Working Memory Network in Schizophrenia
    Authors: Kenneth N. Fish, Brad R. Rocco, Adam M. DeDionisio, Samuel J. Dienel, Robert A. Sweet, David A. Lewis
    Biological Psychiatry
  2. Dorsal striatum c-Fos activity in perseverative ephrin-A2A5−/− mice and the cellular effect of low-intensity rTMS
    Authors: Maitri Tomar, Jennifer Rodger, Jessica Moretti
    Frontiers in Neural Circuits
  3. Markedly Lower Glutamic Acid Decarboxylase 67 Protein Levels in a Subset of Boutons in Schizophrenia
    Authors: Brad R. Rocco, David A. Lewis, Kenneth N. Fish
    Biological Psychiatry
  4. Restoration of brain circulation and cellular functions hours post-mortem
    Authors: Zvonimir Vrselja, Stefano G. Daniele, John Silbereis, Francesca Talpo, Yury M. Morozov, André M. M. Sousa et al.
    Nature
  5. Ciliary neuropeptidergic signaling dynamically regulates excitatory synapses in postnatal neocortical pyramidal neurons
    Authors: Lauren Tereshko, Ya Gao, Brian A Cary, Gina G Turrigiano, Piali Sengupta
    eLife
  6. Isolated loss of the AUTS2 long isoform, brain-wide or targeted to Calbindin -lineage cells, generates a specific suite of brain, behavioral and molecular pathologies
    Authors: Song, Y;Seward, CH;Chen, CY;LeBlanc, A;Leddy, AM;Stubbs, L;
    bioRxiv : the preprint server for biology
    Species: Transgenic Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  7. Spatially resolved transcriptomics reveals genes associated with the vulnerability of middle temporal gyrus in Alzheimer's disease
    Authors: S Chen, Y Chang, L Li, D Acosta, Y Li, Q Guo, C Wang, E Turkes, C Morrison, D Julian, ME Hester, DW Scharre, C Santiskulv, SX Song, JT Plummer, GE Serrano, TG Beach, KE Duff, Q Ma, H Fu
    Acta neuropathologica communications, 2022-12-21;10(1):188.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  8. The beta2V287L nicotinic subunit linked to sleep-related epilepsy differently affects fast-spiking and regular spiking somatostatin-expressing neurons in murine prefrontal cortex
    Authors: S Meneghini, D Modena, G Colombo, A Coatti, N Milani, L Madaschi, A Amadeo, A Becchetti
    Progress in neurobiology, 2022-05-02;0(0):102279.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  9. Regulation of prefrontal patterning and connectivity by retinoic acid
    Authors: M Shibata, K Pattabiram, B Lorente-Ga, D Andrijevic, SK Kim, N Kaur, SK Muchnik, X Xing, G Santpere, AMM Sousa, N Sestan
    Nature, 2021-10-01;0(0):.
    Species: Human, Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  10. Alterations in a Unique Class of Cortical Chandelier Cell Axon Cartridges in Schizophrenia
    Authors: Kenneth N Fish
    Biol. Psychiatry, 2016-09-29;0(0):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  11. Transporters MCT8 and OATP1C1 maintain murine brain thyroid hormone homeostasis.
    Authors: Mayerl S, Muller J, Bauer R, Richert S, Kassmann C, Darras V, Buder K, Boelen A, Visser T, Heuer H
    J Clin Invest, 2014-04-01;124(5):1987-99.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  12. Locomotor deficiencies and aberrant development of subtype-specific GABAergic interneurons caused by an unliganded thyroid hormone receptor alpha1.
    Authors: Wallis K, Sjogren M, van Hogerlinden M, Silberberg G, Fisahn A, Nordstrom K, Larsson L, Westerblad H, Morreale de Escobar G, Shupliakov O, Vennstrom B
    J. Neurosci., 2008-02-20;28(8):1904-15.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  13. The 7q11.23 Protein DNAJC30 Interacts with ATP Synthase and Links Mitochondria to Brain Development.
    Authors: Tebbenkamp ATN, Varela L, Choi J et al.
    Cell.

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Reviews for Human/Mouse/Rat GAD1/GAD67 Antibody

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Human/Mouse/Rat GAD1/GAD67 Antibody
By Anonymous on 04/28/2023
Application: Immunocytochemistry/Immunofluorescence Sample Tested: Adult brain (hippocampus) Species: Mouse

Does not work with incubation at 1:500 to 1:1000 dilutions overnight plus secondary AlexaFluor (multiple tested). No fluorescence detected. Sample tissue fixed with PFA.