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Human/Mouse/Rat Galectin-3 Antibody

Catalog #: AF1197
21 Citations Datasheet / COA / SDS
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AF1197
AF1197-SP
Detection of Mouse Galectin‑3 by Western Blot.
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Citations (21)
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Human/Mouse/Rat Galectin-3 Antibody Summary

Species Reactivity
Human, Mouse, Rat
Specificity
Detects human, mouse, and rat Galectin-3 in Western blots. Detects mouse Galectin-3 in direct ELISAs. In direct ELISAs, approximately 20% cross-reactivity with recombinant human (rh) Galectin-3 is observed and less than 1% cross-reactivity with recombinant mouse (rm) Galectin-4 and rmGalectin-9 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant mouse Galectin‑3
Ala2-Ile264
Accession # NP_034835
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.05-0.2 µg/mL
See below
Simple Western
10 µg/mL
See below
Immunohistochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Mouse Galectin-3 antibody by Western Blot. View Larger

Detection of Mouse Galectin‑3 by Western Blot. Western blot shows lysates of 4T1 mouse breast cancer cell line, Balb/3T3 mouse embryonic fibroblast cell line and HT-2 mouse T cell line. PVDF membrane was probed with 0.05 µg/mL of Goat Anti-Mouse Galectin-3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1197) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Galectin-3 at approximately 28 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Western Blot Detection of Human and Rat Galectin-3 antibody by Western Blot. View Larger

Detection of Human and Rat Galectin‑3 by Western Blot. Western blot shows lysates of MCF-7 human breast cancer cell line, U-118-MG human glioblastoma/astrocytoma cell line, C6 rat glioma cell line, and NR8383 rat alveolar macrophage cell line. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Mouse Galectin-3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1197) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Galectin-3 at approximately 28 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry Galectin‑3 antibody in Mouse Embryo by Immunohistochemistry (IHC-Fr). View Larger

Galectin‑3 in Mouse Embryo. Galectin-3 was detected in immersion fixed frozen sections of mouse embryo (15 d.p.c., cross-section through the eye) using Goat Anti-Human Galectin-3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1197 ) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Simple Western Detection of Mouse Galectin-3 antibody by Simple Western<sup>TM</sup>. View Larger

Detection of Mouse Galectin‑3 by Simple WesternTM. Simple Western lane view shows lysates of HT-2 mouse T cell line and Balb/3T3 mouse embryonic fibroblast cell line, loaded at 0.2 mg/mL. A specific band was detected for Galectin-3 at approximately 44 kDa (as indicated) using 10 µg/mL of Goat Anti-Mouse Galectin-3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1197) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Galectin-3

The galectins constitute a large family of carbohydrate-binding proteins with specificity for N-acetyl-lactosamine-containing glycoproteins. At least 14 mammalian galectins, which share structural similarities in their carbohydrate recognition domains (CRD), have been identified. The galectins have been classified into the prototype galectins (-1, -2, -5, -7, -10, -11, -13, -14), which contain one CRD and exist either as a monomer or a noncovalent homodimer; the chimera galectins (Galectin-3) containing one CRD linked to a nonlectin domain; and the tandem-repeat galectins (-4, -6, -8, -9, -12) consisting of two CRDs joined by a linker peptide. Galectins lack a classical signal peptide and can be localized to the cytosolic compartments where they have intracellular functions. However, via one or more as yet unidentified non-classical secretory pathways, galectins can also be secreted to function extracellularly. Individual members of the galectin family have different tissue distribution profiles and exhibit subtle differences in their carbohydrate-binding specificities. Each family member may preferentially bind to a unique subset of cell-surface glycoproteins (1-4).

Galectin-3, also known as Mac-2, L29, CBP35, and epsilon BP, is a chimera galectin that has a tendency to dimerize. Besides the soluble protein, alternatively spliced forms of chicken Galectin-3 containing a transmembrane-spanning domain and a leucine zipper motif have been reported. Galectin-3 is expressed in tumor cells, macrophages, activated T cells, osteoclasts, epithelial cells, and fibroblasts. It binds various matrix glycoproteins including laminin, fibronectin, LAMPS, 90K/Mac-2BP, MP20, and CEA. Galectin-3 promotes cell growth and proliferation for many cell types. Galectin-3 acts intracellularly to prevent apoptosis. Depending on the cell types, Galectin-3 exhibits pro- or anti-adhesive properties. Galectin-3 has proinflammatory activities in vitro and in vivo. It induces pro-inflammatory and inhibits Th2 type cytokine production. Galectin-3 chemoattracts monocytes and macrophages. It activates and degranulates basophils and mast cells. Elevated circulating levels of Galectin-3 has been show to correlate with the malignant potential of several types of cancer, suggesting that Galectin-3 is also involved in tumor growth and metastasis. Human and mouse Galectin-3 shares approximately 80% amino acid sequence similarity (1-5).

References
  1. Rabinovich, A. et al. (2002) Trends in Immunol. 23:313.
  2. Rabinovich, A. et al. (2002) J. Leukocyte Biology 71:741.
  3. Hughes, R.C. (2001) Biochimie 83:667.
  4. R&D Systems Cytokine Bulletin; Summer 2002.
  5. Gorski, J.P. et al. (2002) J. Biol. Chem. 277:18840.
Entrez Gene IDs
3958 (Human); 16854 (Mouse)
Alternate Names
AGE-R3; Carbohydrate-binding protein 35; CBP 35,35 kDa lectin; CBP35; GAL3; Gal-3; galactin-3; Galactose-specific lectin 3; Galactoside-binding protein; GALBPCBP35; Galectin3; Galectin-3; GALIG; IgE-binding protein; L29; L31; L-31; Laminin-binding protein; Lectin L-29; lectin, galactoside-binding, soluble, 3; LGALS2; LGALS3; Mac-2 antigen; Mac-2; MAC2GAL3

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Citations for Human/Mouse/Rat Galectin-3 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

21 Citations: Showing 1 - 10
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  1. Mutation of the galectin-3 glycan-binding domain (Lgals3-R200S) enhances cortical bone expansion in male mice and trabecular bone mass in female mice
    Authors: KA Maupin, CR Diegel, PD Stevens, D Dick, VAI Vivari, BO Williams
    FEBS Open Bio, 2022-09-14;0(0):.
    Species: Transgenic Mouse
    Sample Types: Cell Lysates, Whole Cells
    Applications: ICC, Western Blot
  2. Regulating microglial miR-155 transcriptional phenotype alleviates Alzheimer's-induced retinal vasculopathy by limiting Clec7a/Galectin-3+ neurodegenerative microglia
    Authors: H Shi, Z Yin, Y Koronyo, DT Fuchs, J Sheyn, MR Davis, JW Wilson, MA Margeta, KM Pitts, S Herron, S Ikezu, T Ikezu, SL Graham, VK Gupta, KL Black, M Mirzaei, O Butovsky, M Koronyo-Ha
    Acta neuropathologica communications, 2022-09-08;10(1):136.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  3. Crosstalk between dihydroceramides produced by Porphyromonas gingivalis and host lysosomal cathepsin B in the promotion of osteoclastogenesis
    Authors: C Duarte, C Yamada, C Garcia, J Akkaoui, A Ho, F Nichols, A Movila
    Journal of Cellular and Molecular Medicine, 2022-04-16;0(0):.
    Species: Mouse
    Sample Types: Cell Lysates, Whole Cells
    Applications: IFC, Western Blot
  4. Astrocytic phagocytosis contributes to demyelination after focal cortical ischemia in mice
    Authors: T Wan, W Zhu, Y Zhao, X Zhang, R Ye, M Zuo, P Xu, Z Huang, C Zhang, Y Xie, X Liu
    Nature Communications, 2022-03-03;13(1):1134.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  5. Gal3 Plays a Deleterious Role in a Mouse Model of Endotoxemia
    Authors: JC Fernández-, AM Espinosa-O, I García-Dom, I Rosado-Sán, YM Pacheco, R Moyano, JG Monterde, JL Venero, RM de Pablos
    International Journal of Molecular Sciences, 2022-01-21;23(3):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  6. Age-related changes in brain phospholipids and bioactive lipids in the APP knock-in mouse model of Alzheimer's disease
    Authors: C Emre, KV Do, B Jun, E Hjorth, SG Alcalde, MI Kautzmann, WC Gordon, P Nilsson, NG Bazan, M Schultzber
    Acta neuropathologica communications, 2021-06-29;9(1):116.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  7. Diurnal Photoreceptor Outer Segment Renewal in Mice Is Independent of Galectin-3
    Authors: NJ Esposito, F Mazzoni, JA Vargas, SC Finnemann
    Investigative Ophthalmology & Visual Science, 2021-02-01;62(2):7.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  8. Network analysis of the progranulin-deficient mouse brain proteome reveals pathogenic mechanisms shared in human frontotemporal dementia caused by GRN mutations
    Authors: M Huang, E Modeste, E Dammer, P Merino, G Taylor, DM Duong, Q Deng, CJ Holler, M Gearing, D Dickson, NT Seyfried, T Kukar
    Acta Neuropathol Commun, 2020-10-07;8(1):163.
    Species: Mouse
    Sample Types: Protein, Whole Tissue
    Applications: IHC-P, Western Blot
  9. Serum amyloid A-containing HDL binds adipocyte-derived versican and macrophage-derived biglycan, reducing its anti-inflammatory properties
    Authors: CY Han, I Kang, M Omer, S Wang, T Wietecha, TN Wight, A Chait
    JCI Insight, 2020-09-24;0(0):.
    Species: Human, Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  10. Plasma APE1/Ref-1 Correlates with Atherosclerotic Inflammation in ApoE-/- Mice
    Authors: YR Lee, HK Joo, EO Lee, MS Park, HS Cho, S Kim, H Jin, JO Jeong, CS Kim, BH Jeon
    Biomedicines, 2020-09-21;8(9):.
    Species: Mouse
    Sample Types: Tissue Homogenates, Whole Cells, Whole Tissue
    Applications: ICC, IHC, Western Blot
  11. Adipocyte-Derived Versican and Macrophage-Derived Biglycan Control Adipose Tissue Inflammation in Obesity
    Authors: CY Han, I Kang, IA Harten, JA Gebe, CK Chan, M Omer, KM Alonge, LJ den Hartig, D Gomes Kjer, L Goodspeed, S Subramania, S Wang, F Kim, DE Birk, TN Wight, A Chait
    Cell Rep, 2020-06-30;31(13):107818.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  12. Voluntary running does not reduce neuroinflammation or improve non-cognitive behavior in the 5xFAD mouse model of Alzheimer's disease
    Authors: M Svensson, E Andersson, O Manouchehr, Y Yang, T Deierborg
    Sci Rep, 2020-01-28;10(1):1346.
    Species: Mouse
    Sample Types: Cell Lysates, Whole Tissue
    Applications: IHC, Western Blot
  13. Bush-like integrin filament networks associated with hyaloid vasculature in murine neonate eyes
    Authors: T Iwanaga, J Nio-Kobaya, H Takahashi-
    Biomed. Res., 2019-01-01;40(2):79-85.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  14. Peripheral Inflammation Enhances Microglia Response and Nigral Dopaminergic Cell Death in an in vivo MPTP Model of Parkinson's Disease
    Authors: I García-Dom, K Veselá, J García-Rev, A Carrillo-J, MA Roca-Cebal, M Santiago, RM de Pablos, JL Venero
    Front Cell Neurosci, 2018-11-06;12(0):398.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  15. Acute Complement Inhibition Potentiates Neurorehabilitation and Enhances tPA-Mediated Neuroprotection
    Authors: A Alawieh, M Andersen, DL Adkins, S Tomlinson
    J. Neurosci., 2018-06-19;38(29):6527-6545.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  16. Histochemical characteristics of regressing vessels in the hyaloid vascular system of neonatal mice: Novel implication for vascular atrophy
    Authors: A Kishimoto, S Kimura, J Nio-Kobaya, H Takahashi-, AM Park, T Iwanaga
    Exp. Eye Res., 2018-03-27;172(0):1-9.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  17. Identifying the role of complement in triggering neuroinflammation after traumatic brain injury
    Authors: A Alawieh, EF Langley, S Weber, D Adkins, S Tomlinson
    J. Neurosci., 2018-02-06;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  18. Kr�ppel-like factor 3 (KLF3/BKLF) is required for widespread repression of the inflammatory modulator galectin-3 (Lgals3)
    J Biol Chem, 2016-05-24;0(0):.
    Species: Mouse
    Sample Types: Cell Culture Supernates, Whole Tissue
    Applications: IHC-P, Western Blot
  19. Galectin-1 and galectin-8 have redundant roles in promoting plasma cell formation.
    Authors: Tsai CM, Guan CH, Hsieh HW, Hsu TL, Tu Z, Wu KJ, Lin CH, Lin KI
    J. Immunol., 2011-07-13;187(4):1643-52.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  20. Characterization of RAGE, HMGB1, and S100beta in inflammation-induced preterm birth and fetal tissue injury.
    Authors: Buhimschi CS, Baumbusch MA, Dulay AT, Oliver EA, Lee S, Zhao G, Bhandari V, Ehrenkranz RA, Weiner CP, Madri JA, Buhimschi IA
    Am. J. Pathol., 2009-08-13;175(3):958-75.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  21. Galectin-3 is an amplifier of inflammation in atherosclerotic plaque progression through macrophage activation and monocyte chemoattraction.
    Authors: Papaspyridonos M, McNeill E, de Bono JP, Smith A, Burnand KG, Channon KM, Greaves DR
    Arterioscler. Thromb. Vasc. Biol., 2007-12-20;28(3):433-40.
    Species: Mouse
    Sample Types: Tissue Homogenates, Whole Tissue
    Applications: IHC-P, Western Blot

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