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Human/Mouse/Rat GDF-8/Myostatin Antibody

Catalog #: AF788
11 Citations Datasheet / COA / SDS
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AF788
AF788-SP
Detection of Recombinant Human, Mouse, and Rat GDF-8/Myostatin by Western Blot.
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Citations (11)
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Human/Mouse/Rat GDF-8/Myostatin Antibody Summary

Species Reactivity
Human, Mouse, Rat
Specificity
Detects human, mouse, and rat GDF-8/Myostatin in direct ELISAs and Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant mouse GDF-8/Myostatin
Asp268-Ser376
Accession # O08689
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
See below
Immunohistochemistry
5-15 µg/mL
See below
Neutralization
Measured by its ability to neutralize GDF‑8/Myostatin-induced hemoglobin expression in the K562 human chronic myelogenous leukemia cell line. The Neutralization Dose (ND50) is typically 0.6-3 µg/mL in the presence of 30 ng/mL Recombinant Mouse GDF‑8/Myostatin.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Recombinant Human, Mouse, and Rat GDF-8/Myostatin antibody by Western Blot. View Larger

Detection of Recombinant Human, Mouse, and Rat GDF-8/Myostatin by Western Blot. Western blot shows 25 ng of Recombinant Human/Mouse/Rat GDF-8/Myostatin (788-G8), Recombinant Human/Mouse/Rat GDF-11/BMP-11 (Catalog # 1958-GD), Recombinant Human BMP-6 (507-BP), and Recombinant Mouse BMP-6 (Catalog # 6325-BM). PVDF Membrane was probed with 0.1 µg/mL of Goat Anti-Human/Mouse/Rat GDF-8/Myostatin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF788) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for GDF-8/Myostatin at approximately 14 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.

Immunohistochemistry GDF-8/Myostatin antibody in Mouse Embryo by Immunohistochemistry (IHC-Fr). View Larger

GDF‑8/Myostatin in Mouse Embryo. GDF-8/Myostatin was detected in immersion fixed frozen sections of mouse embryo (10 d.p.c., section through neural tube) using Goat Anti-Human/Mouse/Rat GDF-8/Myostatin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF788) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Neutralization Hemoglobin Expression Induced by GDF‑8/Myostatin and Neutralization by Mouse GDF‑8/Myostatin Antibody. View Larger

Hemoglobin Expression Induced by GDF‑8/Myostatin and Neutralization by Mouse GDF‑8/Myostatin Antibody. Recombinant Mouse GDF-8/Myostatin (Catalog # 788-G8) increases hemoglobin expression in the K562 human chronic myelogenous leukemia cell line in a dose-dependent manner (orange line), as measured by the psuedoperoxidase assay. Hemoglobin expression elicited by Recombinant Mouse GDF-8/Myostatin (30 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human/Mouse/Rat GDF-8/Myostatin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF788). The ND50 is typically 0.6-3 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GDF-8/Myostatin

Growth Differentiation Factor 8 (GDF-8), also known as myostatin, is a member of the TGF-beta superfamily that is expressed specifically in developing and adult skeletal muscle. GDF-8 cDNA encodes a 376 amino acid (aa) prepropeptide with a 24 aa residue signal peptide, a 223 aa residue amino-terminal propeptide, and a 109 aa residue carboxy-terminal mature protein. Mature GDF-8 contains the canonical 7-cysteine motif common to other TGF-beta superfamily members. Similar to the TGF-beta s, activins and BMP-11, GDF-8 also contains one extra pair of cysteine residues that is not found in other family members. The bioactive form of GDF-8 is a homodimer with an apparent molecular weight of approximately 25 kDa. GDF-8 is highly conserved across species. At the amino acid sequence level, mature human, mouse, rat and cow GDF-8 are 100% identical. Within the TGF-beta superfamily, GDF-8 is most closely related to BMP-11, a mammalian protein that acts as a dorsal mesoderm and neural inducer in Xenopus explants. The two proteins share 90% amino acid sequence identity within their mature chain. A targeted disruption of GDF-8 in mouse results in large mice with a widespread increase in skeletal muscle mass, indicating that GDF-8 is a negative regulator of skeletal muscle growth. A mutation in the bovine GDF-8 gene has been shown to be responsible for the double-muscled phenotype in cattle breeds such as Belgian Blue cattle that is characterized by an increase in muscle mass. GDF-8 has also been shown to inhibit preadipocyte differentiation to adipocytes. Mature GDF-8 binds to activin type II receptors and the binding is antagonized by the activin-binding protein, follistatin. R&D Systems recombinant GDF-8 preparations have been shown to act similarly to Activin A in both the Xenopus animal cap and the K562 assays.

References
  1. Storm, E.E. et al. (1994) Nature 368:639.
  2. Sharma, M. et al. (1999) J. Cell Physiol. 180:1.
  3. McPherron, A.C. et al. (1997) Nature 387:83.
  4. Lee, S.J. et al. (2001) Proc. Natl. Acad. Sci. USA 98:9306.
  5. Kim, H.S. et al. (2001) Biochem. Biophys. Res. Commun. 281:902.
Long Name
Growth Differentiation Factor 8
Entrez Gene IDs
2660 (Human); 17700 (Mouse)
Alternate Names
GDF8; GDF-8; GDF8growth differentiation factor 8; growth/differentiation factor 8; MSLHP; MSTN; Myostatin

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Citations for Human/Mouse/Rat GDF-8/Myostatin Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

11 Citations: Showing 1 - 10
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  1. The relationship between myodural bridges, hyperplasia of the suboccipital musculature, and intracranial pressure
    Authors: C Li, C Yue, ZC Liu, J Gong, XS Wei, H Yang, C Gilmore, SB Yu, GD Hack, HJ Sui
    PLoS ONE, 2022-09-02;17(9):e0273193.
    Species: Rat
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  2. Novel myostatin-specific antibody enhances muscle strength in muscle disease models
    Authors: H Muramatsu, T Kuramochi, H Katada, A Ueyama, Y Ruike, K Ohmine, M Shida-Kawa, R Miyano-Nis, Y Shimizu, M Okuda, Y Hori, M Hayashi, K Haraya, N Ban, T Nonaka, M Honda, H Kitamura, K Hattori, T Kitazawa, T Igawa, Y Kawabe, J Nezu
    Scientific Reports, 2021-01-25;11(1):2160.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  3. A GDF11/myostatin inhibitor, GDF11 propeptide-Fc, increases skeletal muscle mass and improves muscle strength in dystrophic mdx mice
    Authors: Q Jin, C Qiao, J Li, B Xiao, J Li, X Xiao
    Skelet Muscle, 2019-05-27;9(1):16.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  4. Impact of heat therapy on recovery after eccentric exercise in humans
    Authors: Kyoungrae Kim, Shihuan Kuang, Qifan Song, Timothy P. Gavin, Bruno T. Roseguini
    Journal of Applied Physiology
    Species: Human
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  5. Inhibition of GDF8 (Myostatin) accelerates bone regeneration in diabetes mellitus type 2
    Authors: C Wallner, H Jaurich, JM Wagner, M Becerikli, K Harati, M Dadras, M Lehnhardt, B Behr
    Sci Rep, 2017-08-29;7(1):9878.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  6. Compensatory anabolic signaling in the sarcopenia of experimental chronic arthritis
    Authors: RD Little, I Prieto-Pot, S Pérez-Baos, A Villalvill, P Gratal, F Cicuttini, R Largo, G Herrero-Be
    Sci Rep, 2017-07-24;7(1):6311.
    Species: Rabbit
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  7. Myostatin signaling is up-regulated in female patients with advanced heart failure
    Authors: J Ishida, M Konishi, M Saitoh, M Anker, SD Anker, J Springer
    Int. J. Cardiol., 2017-04-04;0(0):.
    Species: Human
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  8. The Use of Platelet-Rich and Platelet-Poor Plasma to Enhance Differentiation of Skeletal Myoblasts: Implications for the Use of Autologous Blood Products for Muscle Regeneration
    Authors: O Miroshnych, WT Chang, JL Dragoo
    Am J Sports Med, 2016-12-27;45(4):945-953.
    Species: Human
    Sample Types: Protein
    Applications: Western Blot
  9. A single heterochronic blood exchange reveals rapid inhibition of multiple tissues by old blood
    Nat Commun, 2016-11-22;7(0):13363.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  10. High concentrations of HGF inhibit skeletal muscle satellite cell proliferation in vitro by inducing expression of myostatin: a possible mechanism for reestablishing satellite cell quiescence in vivo.
    Authors: Yamada M, Tatsumi R, Yamanouchi K, Hosoyama T, Shiratsuchi S, Sato A, Mizunoya W, Ikeuchi Y, Furuse M, Allen RE
    Am. J. Physiol., Cell Physiol., 2009-12-09;298(3):C465-76.
    Species: Rat
    Sample Types: Whole Cells
    Applications: ICC
  11. Increased secretion and expression of myostatin in skeletal muscle from extremely obese women.
    Authors: Hittel DS, Berggren JR, Shearer J, Boyle K, Houmard JA
    Diabetes, 2008-10-03;58(1):30-8.
    Species: Human
    Sample Types: Tissue Homogenates
    Applications: Western Blot

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