Human/Mouse/Rat HIF-1 alpha/HIF1A Antibody Summary
Arg575-Asn826
Accession # Q16665.1
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
Detection of Human, Mouse, and Rat HIF-1 alpha/HIF1A by Western Blot. Western blot shows lysates of MCF-7 human breast cancer cell line, Balb-3T3 mouse embryonic fibroblast cell line, and PC-12 rat adrenal pheochromocytoma cell line untreated (-) or treated (+) with 150 µM CoCl2for 8 hours. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse/Rat HIF-1 alpha/HIF1A Monoclonal Antibody, followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for HIF-1 alpha/HIF1A at approximately 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
HIF-1 alpha/HIF1A in Human Kidney. HIF-1 alpha/HIF1A was detected in immersion fixed paraffin-embedded sections of human kidney using Mouse Anti-Human/Mouse/Rat HIF-1 alpha/HIF1A Monoclonal Antibody (Catalog # MAB1536) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm and nuclei. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Western Blot Shows Human HIF-1 alpha/HIF1A alpha/HIF1A Specificity by Using Knockout Cell Line. Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and HIF-1 alpha/HIF1A knockout HeLa cell line (KO) untreated (-) or treated (+) with 1 mM DFO overnight. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse/Rat HIF-1 alpha/HIF1A Monoclonal Antibody (Catalog # MAB1536) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for HIF-1 alpha/HIF1A at approximately 110 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # MAB5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human HIF-1 alpha/HIF1A by Simple WesternTM. Simple Western lane view shows lysates of A549 human lung carcinoma cell line untreated (-) or treated (+) with Hypoxia (1% O2), loaded at 0.2 mg/mL. A specific band was detected for HIF-1 alpha/HIF1A at approximately 116 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human/Mouse/Rat HIF-1 alpha/HIF1A Monoclonal Antibody (Catalog # MAB1536). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
HIF-1 alpha/HIF1A Specificity is Shown by Immunocytochemistry in Knockout Cell Line. HIF-1 alpha/HIF1A was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line treated with DFO but is not detected in HIF-1 alpha/HIF1A knockout (KO) HeLa Human Cell Line cell line using Mouse Anti-Human/Mouse/Rat HIF-1 alpha/HIF1A alpha/HIF1A Monoclonal Antibody (Catalog # MAB1536) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: HIF-1 alpha/HIF1A
The hypoxia-inducible transcription factor 1 alpha (HIF-1 alpha ) is the regulated member of the transcription factor heterodimer HIF-1. HIF-1 binds to hypoxia-response elements (HREs) in the promoters of many genes involved in adapting to an environment of insufficient oxygen or hypoxia. Hypoxic tissue environments occur in vascular and pulmonary diseases as well as cancer, which illustrates the broad impact of gene regulation by HIF-1 alpha.
Product Datasheets
Citations for Human/Mouse/Rat HIF-1 alpha/HIF1A Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 10
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Stearoyl-CoA Desaturase-1 dependent lipid droplets accumulation in cancer-associated fibroblasts facilitates the progression of lung cancer
Authors: Y Zhang, Z Gu, J Wan, X Lou, S Liu, Y Wang, Y Bian, F Wang, Z Li, Z Qin
International journal of biological sciences, 2022-10-18;18(16):6114-6128.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
Renal organoid modeling of tuberous sclerosis complex reveals lesion features arise from diverse developmental processes
Authors: A Pietrobon, J Yockell-Le, TA Flood, WL Stanford
Cell Reports, 2022-07-05;40(1):111048.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
The study of direct and indirect effects of radiofrequency ablation on tumor microenvironment in liver tumor animal model
Authors: AN Jiang, B Wang, S Wang, K Zhao, H Wu, K Yan, W Wu, W Yang
BMC Cancer, 2022-06-17;22(1):663.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
MSC Pretreatment for Improved Transplantation Viability Results in Improved Ventricular Function in Infarcted Hearts
Authors: MF Pittenger, S Eghtesad, PG Sanchez, X Liu, Z Wu, L Chen, BP Griffith
International Journal of Molecular Sciences, 2022-01-08;23(2):.
Species: Rat
Sample Types: Cell Lysates
Applications: Western Blot -
Differentiation of cancer stem cells into erythroblasts in the presence of CoCl2
Authors: K Kumon, SM Afify, G Hassan, S Ueno, S Monzur, HM Nawara, HAA Quora, M Sheta, Y Xu, X Fu, MH Zahra, A Seno, M Seno
Scientific Reports, 2021-12-14;11(1):23977.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
HDAC inhibitors stimulate LIFR when it is repressed by hypoxia or PTHrP in breast cancer
Authors: CM Edwards, ME Clements, LA Vecchi, JA Johnson, RW Johnson
Oncogene, 2021-12-01;31(0):100407.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
mTORC1-induced retinal progenitor cell overproliferation leads to accelerated mitotic aging and degeneration of descendent M�ller glia
Authors: S Lim, YJ Kim, S Park, JH Choi, Y Sung, K Nishimori, Z Kozmik, HW Lee, JW Kim
Elife, 2021-10-22;10(0):.
Species: Mouse
Sample Types: Tissue Lysates
Applications: Western Blot -
Stem cell factor Is implicated In microenvironmental interactions and cellular dynamics of chronic lymphocytic leukemia
Authors: GI Gavriilidi, S Ntoufa, N Papakonsta, K Kotta, T Koletsa, E Chartomats, T Moysiadis, N Stavroyian, A Anagnostop, E Papadaki, AS Tsiftsoglo, K Stamatopou
Haematologica, 2021-03-01;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
&gamma-Glutamylcyclotransferase, a novel regulator of HIF-1&alpha expression, triggers aerobic glycolysis
Authors: K Taniguchi, S Kageyama, C Moyama, S Ando, H Ii, E Ashihara, M Horinaka, T Sakai, S Kubota, A Kawauchi, S Nakata
Cancer Gene Therapy, 2021-01-05;0(0):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Prolonged astrocyte-derived erythropoietin expression attenuates neuronal damage under hypothermic conditions
Authors: K Toriuchi, H Kakita, T Tamura, S Takeshita, Y Yamada, M Aoyama
J Neuroinflammation, 2020-05-02;17(1):141.
Species: Rat
Sample Types: Cell Lysate
Applications: Western Blot -
pVHL-mediated regulation of the anti-angiogenic protein thrombospondin-1 decreases migration of Clear Cell Renal Carcinoma Cell Lines
Authors: J Sevilla-Mo, R Bienes-Mar, D Labrousse-, E Fuertes-Ye, Á Ordóñez, MJ Calzada
Sci Rep, 2020-01-24;10(1):1175.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Interaction of Pregnancy-Specific Glycoprotein 1 With Integrin alpha5beta1 Is a Modulator of Extravillous Trophoblast Functions
Authors: S Rattila, CEE Dunk, M Im, O Grichenko, Y Zhou, M Yanez-Mo, SM Blois, KM Yamada, O Erez, N Gomez-Lope, SJ Lye, B Hinz, R Romero, M Cohen, G Dveksler
Cells, 2019-10-31;8(11):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Modeling radiation injury-induced cell death and countermeasure drug responses in a human Gut-on-a-Chip
Authors: S Jalili-Fir, R Prantil-Ba, A Jiang, R Potla, T Mammoto, JC Weaver, TC Ferrante, HJ Kim, JMS Cabral, O Levy, DE Ingber
Cell Death Dis, 2018-02-14;9(2):223.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
XIAP upregulates expression of HIF target genes by targeting HIF1? for Lys63-linked polyubiquitination
Authors: CV Park, IG Ivanova, NS Kenneth
Nucleic Acids Res., 2017-09-19;0(0):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Protective effect of zinc preconditioning against renal ischemia reperfusion injury is dose dependent
Authors: K Rao, K Sethi, J Ischia, L Gibson, L Galea, L Xiao, M Yim, M Chang, N Papa, D Bolton, A Shulkes, GS Baldwin, O Patel
PLoS ONE, 2017-07-07;12(7):e0180028.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Silencing HIF-1? induces TET2 expression and augments ascorbic acid induced 5-hydroxymethylation of DNA in human metastatic melanoma cells
Authors: AP Fischer, SL Miles
Biochem. Biophys. Res. Commun., 2017-06-08;0(0):.
Species: Human
Sample Types: Protein
Applications: Western Blot -
Ferrochelatase is a therapeutic target for ocular neovascularization
Authors: HD Basavaraja, RS Sulaiman, X Qi, T Shetty, S Sheik Pran, KL Sishtla, B Lee, J Quigley, S Alkhairy, CM Briggs, K Gupta, B Tang, M Shadmand, MB Grant, ME Boulton, SY Seo, TW Corson
EMBO Mol Med, 2017-06-01;0(0):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Hybrid Cellular Metabolism Coordinated by Zic3 and Esrrb Synergistically Enhances Induction of Naive Pluripotency
Authors: M Sone, N Morone, T Nakamura, A Tanaka, K Okita, K Woltjen, M Nakagawa, JE Heuser, Y Yamada, S Yamanaka, T Yamamoto
Cell Metab., 2017-05-02;25(5):1103-1117.e6.
Species: Mouse
Sample Types: Whole Cells
Applications: ICC -
HIF1? regulates single differentiated glioma cell dedifferentiation to stem-like cell phenotypes with high tumorigenic potential under hypoxia
Authors: P Wang, C Lan, S Xiong, X Zhao, Y Shan, R Hu, W Wan, S Yu, B Liao, G Li, J Wang, D Zou, B Chen, H Feng, N Wu
Oncotarget, 2017-04-25;8(17):28074-28092.
Species: Human
Sample Types: Cell Lysates, Whole Cells
Applications: ICC, Western Blot -
Mitochondrial complex I deactivation is related to superoxide production in acute hypoxia
Authors: P Hernansanz, E Ramos, E Navarro, E Parada, N Sánchez-Ló, L Peláez-Agu, JD Cabrera-Ga, D Tello, I Buendia, A Marina, J Egea, MG López, A Bogdanova, A Martínez-R
Redox Biol, 2017-04-21;12(0):1040-1051.
Species: Bovine
Sample Types: Cell Lysates
Applications: Western Blot -
Metabolic changes and inflammation in cultured astrocytes from the 5xFAD mouse model of Alzheimer's disease: Alleviation by pantethine
Authors: M van Gijsel, K Baranger, P Benech, S Rivera, M Khrestchat, M de Reggi, B Gharib
PLoS ONE, 2017-04-14;12(4):e0175369.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
VHL promotes immune response against renal cell carcinoma via NF-?B-dependent regulation of VCAM-1
Authors: D Labrousse-, E Martínez-A, M Corral-Esc, R Bienes-Mar, J Berridy, L Serrano-Ov, E Conde, ML García-Ber, JM Giménez-Ba, AS Salinas-Sá, R Sánchez-Pr, M Yao, M Lasa, MJ Calzada
J. Cell Biol, 2017-02-24;0(0):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
NQO1 inhibits proteasome-mediated degradation of HIF-1?
Nat Commun, 2016-12-14;7(0):13593.
Species: Human
Sample Types: Cell Lysates, Whole Cells, Whole Tissue
Applications: ICC, IHC-P, Western Blot -
Chronic intermittent hypoxia induces cardiac inflammation and dysfunction in a rat obstructive sleep apnea model
Authors: Qin Wei
J Biomed Res, 2016-12-12;30(6):490-495.
Species: Rat
Sample Types: Tissue Homogenates
Applications: Western Blot -
Phosphoribosylation of Ubiquitin Promotes Serine Ubiquitination and Impairs Conventional Ubiquitination
Authors: Sagar Bhogaraju
Cell, 2016-12-01;167(6):1636-1649.e13.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Leptin directly promotes T-cell glycolytic metabolism to drive effector T-cell differentiation in a mouse model of autoimmunity
Eur J Immunol, 2016-06-13;0(0):.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
Dose-dependent effects of allopurinol on human foreskin fibroblast cells and human umbilical vein endothelial cells under hypoxia.
Authors: Sun Y, George J, Rocha S
PLoS ONE, 2015-04-01;10(4):e0123649.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Panobinostat reduces hypoxia-induced cisplatin resistance of non-small cell lung carcinoma cells via HIF-1alpha destabilization.
Authors: Fischer C, Leithner K, Wohlkoenig C, Quehenberger F, Bertsch A, Olschewski A, Olschewski H, Hrzenjak A
Mol Cancer, 2015-01-21;14(1):4.
Species: Human
Sample Types: Whole Cells
Applications: IHC-Fr -
Triptolide reverses hypoxia-induced epithelial-mesenchymal transition and stem-like features in pancreatic cancer by NF-kappaB downregulation.
Authors: Liu L, Salnikov A, Bauer N, Aleksandrowicz E, Labsch S, Nwaeburu C, Mattern J, Gladkich J, Schemmer P, Werner J, Herr I
Int J Cancer, 2014-05-15;134(10):2489-503.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
HIF-1-PHD2 axis controls expression of syndecan 4 in nucleus pulposus cells.
Authors: Fujita N, Hirose Y, Tran C, Chiba K, Miyamoto T, Toyama Y, Shapiro I, Risbud M
FASEB J, 2014-02-20;28(6):2455-65.
Species: Human, Rat
Sample Types: Cell Lysates
Applications: Western Blot -
NF-kappaB enhances hypoxia-driven T-cell immunosuppression via upregulation of adenosine A(2A) receptors.
Authors: Bruzzese L, Fromonot J, By Y, Durand-Gorde J, Condo J, Kipson N, Guieu R, Fenouillet E, Ruf J
Cell Signal, 2014-01-29;26(5):1060-7.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
The P-body component USP52/PAN2 is a novel regulator of HIF1A mRNA stability.
Authors: Bett J, Ibrahim A, Garg A, Kelly V, Pedrioli P, Rocha S, Hay R
Biochem J, 2013-04-15;451(2):185-94.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Evolutionary conserved regulation of HIF-1beta by NF-kappaB.
Authors: van Uden P, Kenneth NS, Webster R, Muller HA, Mudie S, Rocha S
PLoS Genet., 2011-01-27;7(1):e1001285.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Hypoxic preconditioning advances CXCR4 and CXCR7 expression by activating HIF-1alpha in MSCs.
Authors: Liu H, Xue W, Ge G, Luo X, Li Y, Xiang H, Ding X, Tian P, Tian X
Biochem. Biophys. Res. Commun., 2010-09-24;401(4):509-15.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
The von Hippel-Lindau Chuvash mutation promotes pulmonary hypertension and fibrosis in mice.
Authors: Hickey MM, Richardson T, Wang T, Mosqueira M, Arguiri E, Yu H, Yu QC, Solomides CC, Morrisey EE, Khurana TS, Christofidou-Solomidou M, Simon MC
J. Clin. Invest., 2010-02-08;120(3):827-39.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot -
CYR61 and alphaVbeta5 integrin cooperate to promote invasion and metastasis of tumors growing in preirradiated stroma.
Authors: Monnier Y, Farmer P, Bieler G, Imaizumi N, Sengstag T, Alghisi GC, Stehle JC, Ciarloni L, Andrejevic-Blant S, Moeckli R, Mirimanoff RO, Goodman SL, Delorenzi M, Ruegg C
Cancer Res., 2008-09-15;68(18):7323-31.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Oxygen tension regulates preosteocyte maturation and mineralization.
Authors: Zahm AM, Bucaro MA, Srinivas V, Shapiro IM, Adams CS
Bone, 2008-03-29;43(1):25-31.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
Nucleus pulposus cells express HIF-1alpha under normoxic culture conditions: a metabolic adaptation to the intervertebral disc microenvironment.
Authors: Risbud MV, Guttapalli A, Stokes DG, Hawkins D, Danielson KG, Schaer TP, Albert TJ, Shapiro IM
J. Cell. Biochem., 2006-05-01;98(1):152-9.
Species: Human, Ovine, Rat
Sample Types: Cell Lysates, Whole Cells
Applications: ICC, Western Blot
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