Human/Mouse/Rat HSP90 Antibody Summary
Met1-Asp724
Accession # P08238
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human/Mouse/Rat HSP90 by Western Blot. Western blot shows lysates of Raji human Burkitt's lymphoma cell line and HeLa human cervical epithelial carcinoma cell line, CH-1 mouse B cell lymphoma cell line, A20 mouse B cell lymphoma cell line, and L6 rat myoblast cell line. PVDF membrane was probed with 0.25 µg/mL Human/Mouse/Rat HSP90 Monoclonal Antibody (Catalog # MAB3286) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). For additional reference, recombinant human HSP90a and HSP90 beta (5 ng/lane) were included. A specific band for HSP90 was detected at approximately 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
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Detection of Human and Rat HSP90 by Simple WesternTM. Simple Western lane view shows lysates of Raji human Burkitt's lymphoma cell line and L6 rat myoblast cell line, loaded at 0.2 mg/mL. A specific band was detected for HSP90 at approximately 95 kDa (as indicated) using 2.5 µg/mL of Mouse Anti-Human/Mouse/Rat HSP90 Monoclonal Antibody (Catalog # MAB3286). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: HSP90
HSP90 is a composite name for a large group of Heat Shock Proteins whose molecular weights average 90 kDa. HSPs functions primarily as a molecular chaperone, facilitating the folding of other cellular proteins, preventing protein aggregation, or targeting improperly folded proteins to specific degradative pathways. HSP90 is a central component in a number of basic cellular processes including hormone signaling and cell cycle control. HSP90 is ubiquitously expressed, highly conserved and accounts for 1‑2% of the total cellular protein. Recently introduced, standardized nomenclature has divided the 17 identified HSP90 genes into three related and one unrelated classes, HSP90AA, HSP90AB, HSP90BB, and TRAP, respectively. Eukaryotic cells have two principal isoforms of HSP90. The antibody described here reacts with the 724 amino acid constitutively expressed HSP90AB1-isoform 1 (also known as HSP90 beta, HSP90B, HSPCB, HSPC2, and HSP89 beta ) and also with the 737 amino acid inducible form, HSP90AA1 (also known as HSP90 alpha, HSP90A, HSPC1, HSPCA, HSP89 alpha and LAP2). HSP90AB1-1 and HSP90AA share 90% identity.
Product Datasheets
Citations for Human/Mouse/Rat HSP90 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 8
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Divergent single cell transcriptome and epigenome alterations in ALS and FTD patients with C9orf72 mutation
Authors: Li J, Jaiswal MK, Chien JF et al.
Nat Commun
Species: Human
Sample Types:
Applications: Simple Western -
Nuclear pore complex-mediated modulation of TCR signaling is required for na�ve CD4+ T cell homeostasis
Authors: J Borlido, S Sakuma, M Raices, F Carrette, R Tinoco, LM Bradley, MA D'Angelo
Nat. Immunol., 2018-05-07;0(0):.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
Nuclear Pores Regulate Muscle Development and Maintenance by Assembling a Localized Mef2C Complex
Authors: M Raices, L Bukata, S Sakuma, J Borlido, LS Hernandez, DO Hart, MA D'Angelo
Dev. Cell, 2017-06-05;41(5):540-554.e7.
Species: Zebrafish
Sample Types: Protein
Applications: Western Blot -
Comparative analysis of the interaction of HSPs in dendritic cells, macrophages, RGM-1 cells infected by Helicobacter pylori
Authors: Guangxin Li
Am J Transl Res, 2016-10-15;8(10):4184-4194.
Species: Rat
Sample Types: Whole Cells
Applications: Western Blot -
Mutation in integrin-linked kinase (ILK(R211A)) and heat-shock protein 70 comprise a broadly cardioprotective complex.
Authors: Traister, Alexandr, Walsh, Mark, Aafaqi, Shabana, Lu, Minglian, Dai, Xiaojing, Henkleman, Mark R, Momen, Abdul, Zhou, Yu-Quing, Husain, Mansoor, Arab, Sara, Piran, Sara, Hannigan, Gregory, Coles, John G
PLoS ONE, 2013-11-18;8(11):e77331.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Glucocorticoids enhance CD163 expression in placental Hofbauer cells.
Authors: Tang Z, Niven-Fairchild T, Tadesse S, Norwitz E, Buhimschi C, Buhimschi I, Guller S
Endocrinology, 2012-11-09;154(1):471-82.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Hypoxia Potentiates MicroRNA-Mediated Gene Silencing through Posttranslational Modification of Argonaute2
Authors: Connie Wu, Jessica So, Brandi N. Davis-Dusenbery, Hank H. Qi, Donald B. Bloch, Yang Shi et al.
Molecular and Cellular Biology
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A Hyper-IgM Syndrome Mutation in Activation-Induced Cytidine Deaminase Disrupts G-Quadruplex Binding and Genome-wide Chromatin Localization
Authors: William T. Yewdell, Youngjun Kim, Priyanka Chowdhury, Colleen M. Lau, Ryan M. Smolkin, Kalina T. Belcheva et al.
Immunity
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