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Human/Mouse/Rat HSP90 Antibody

Catalog #: MAB3286
5 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB3286
MAB3286-SP
Detection of Human/Mouse/Rat HSP90 by Western Blot.
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Citations (5)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse/Rat HSP90 Antibody Summary

Species Reactivity
Human, Mouse, Rat
Specificity
Detects human, mouse and rat HSP90 in direct ELISAs and Western blots. Reacts with both recombinant human HSP90 alpha and HSP90 beta isoforms in direct ELISAs and Western blots.
Source
Monoclonal Mouse IgG2B Clone # 341320
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human HSP90 beta
Met1-Asp724
Accession # P08238
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.25 µg/mL
See below
Simple Western
2.5 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human/Mouse/Rat HSP90 antibody by Western Blot. View Larger

Detection of Human/Mouse/Rat HSP90 by Western Blot. Western blot shows lysates of Raji human Burkitt's lymphoma cell line and HeLa human cervical epithelial carcinoma cell line, CH-1 mouse B cell lymphoma cell line, A20 mouse B cell lymphoma cell line, and L6 rat myoblast cell line. PVDF membrane was probed with 0.25 µg/mL Human/Mouse/Rat HSP90 Monoclonal Antibody (Catalog # MAB3286) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). For additional reference, recombinant human HSP90a and HSP90 beta (5 ng/lane) were included. A specific band for HSP90 was detected at approximately 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Simple Western Detection of Human and Rat HSP90 antibody by Simple Western<SUP>TM</SUP>. View Larger

Detection of Human and Rat HSP90 by Simple WesternTM. Simple Western lane view shows lysates of Raji human Burkitt's lymphoma cell line and L6 rat myoblast cell line, loaded at 0.2 mg/mL. A specific band was detected for HSP90 at approximately 95 kDa (as indicated) using 2.5 µg/mL of Mouse Anti-Human/Mouse/Rat HSP90 Monoclonal Antibody (Catalog # MAB3286). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: HSP90

HSP90 is a composite name for a large group of Heat Shock Proteins whose molecular weights average 90 kDa. HSPs functions primarily as a molecular chaperone, facilitating the folding of other cellular proteins, preventing protein aggregation, or targeting improperly folded proteins to specific degradative pathways. HSP90 is a central component in a number of basic cellular processes including hormone signaling and cell cycle control. HSP90 is ubiquitously expressed, highly conserved and accounts for 1‑2% of the total cellular protein. Recently introduced, standardized nomenclature has divided the 17 identified HSP90 genes into three related and one unrelated classes, HSP90AA, HSP90AB, HSP90BB, and TRAP, respectively. Eukaryotic cells have two principal isoforms of HSP90. The antibody described here reacts with the 724 amino acid constitutively expressed HSP90AB1-isoform 1 (also known as HSP90 beta, HSP90B, HSPCB, HSPC2, and HSP89 beta ) and also with the 737 amino acid inducible form, HSP90AA1 (also known as HSP90 alpha, HSP90A, HSPC1, HSPCA, HSP89 alpha and LAP2). HSP90AB1-1 and HSP90AA share 90% identity.

Long Name
Heat Shock Protein 90
Alternate Names
FLJ31884; Heat shock 86 kDa; heat shock 90kD protein 1, alpha; heat shock 90kD protein 1, alpha-like 4; heat shock 90kD protein, alpha-like 4; heat shock 90kDa protein 1, alpha; heat shock protein 90kDa alpha (cytosolic), class A member 1; heat shock protein HSP 90-alpha; Hsp89; HSP90; HSP90AHSP86; HSP90N; HSPC1HSP 86; HSPCAHSP89A; HSPCAL1; HSPCAL4; HSPN; LAP2; Renal carcinoma antigen NY-REN-38

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Citations for Human/Mouse/Rat HSP90 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. Nuclear pore complex-mediated modulation of TCR signaling is required for na�ve CD4+ T cell homeostasis
    Authors: J Borlido, S Sakuma, M Raices, F Carrette, R Tinoco, LM Bradley, MA D'Angelo
    Nat. Immunol., 2018-05-07;0(0):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. Nuclear Pores Regulate Muscle Development and Maintenance by Assembling a Localized Mef2C Complex
    Authors: M Raices, L Bukata, S Sakuma, J Borlido, LS Hernandez, DO Hart, MA D'Angelo
    Dev. Cell, 2017-06-05;41(5):540-554.e7.
    Species: Zebrafish
    Sample Types: Protein
    Applications: Western Blot
  3. Comparative analysis of the interaction of HSPs in dendritic cells, macrophages, RGM-1 cells infected by Helicobacter pylori
    Authors: Guangxin Li
    Am J Transl Res, 2016-10-15;8(10):4184-4194.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Western Blot
  4. Mutation in integrin-linked kinase (ILK(R211A)) and heat-shock protein 70 comprise a broadly cardioprotective complex.
    Authors: Traister, Alexandr, Walsh, Mark, Aafaqi, Shabana, Lu, Minglian, Dai, Xiaojing, Henkleman, Mark R, Momen, Abdul, Zhou, Yu-Quing, Husain, Mansoor, Arab, Sara, Piran, Sara, Hannigan, Gregory, Coles, John G
    PLoS ONE, 2013-11-18;8(11):e77331.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  5. Glucocorticoids enhance CD163 expression in placental Hofbauer cells.
    Authors: Tang Z, Niven-Fairchild T, Tadesse S, Norwitz E, Buhimschi C, Buhimschi I, Guller S
    Endocrinology, 2012-11-09;154(1):471-82.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot

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