Human/Mouse/Rat Integrin alpha V/CD51 Antibody

Catalog #: AF1219
7 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF1219
AF1219-SP

Detection of Human Integrin alpha V/CD51 by Western Blot.

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Citations (7)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse/Rat Integrin alpha V/CD51 Antibody Summary

Species Reactivity

Human, Mouse, Rat

Specificity

Detects human Integrin alpha V in direct ELISAs and Western blots. Detects human, mouse and rat Integrin alpha V in Simple Western application.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human Integrin alpha V/CD51
Phe31-Val992
Accession # P06756

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.5 µg/mL

See below

Simple Western

20 µg/mL

See below

Flow Cytometry

2.5 µg/10⁶ cells

Human peripheral blood mononuclear cells

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Immunocytochemistry

5-15 µg/mL

See below

Knockout Validated

Integrin alpha V/CD51 is specifically detected in A549 human lung carcinoma parental cell line but is not detectable in Integrin alpha V/CD51 knockout A549 cell line.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human Integrin alpha V/CD51 by Western Blot. Western blot shows lysates of HUVEC human umbilical vein endothelial cells, A549 human lung carcinoma cell line, and HT1080 human fibrosarcoma cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human Integrin aV/CD51 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1219) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Integrin aV/CD51 at approximately 130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry

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Integrin alpha V/CD51 in Rat Mesenchymal Stem Cells. Integrin aV/CD51 was detected in immersion fixed undifferentiated rat mesenchymal stem cells using Goat Anti-Human/Mouse/Rat Integrin aV/CD51 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1219) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Simple Western

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Detection of Human, Mouse, and Rat Integrin alpha V/CD51 by Simple Western^TM. Simple Western lane view shows lysates of HUVEC human umbilical vein endothelial cells, A549 human lung carcinoma cell line, C6 rat glioma cell line, and NIH-3T3 mouse embryonic fibroblast cell line, loaded at 0.2 mg/mL. A specific band was detected for Integrin aV/CD51 at approximately 149-161 kDa (as indicated) using 20 µg/mL of Goat Anti-Human/Mouse/Rat Integrin aV/CD51 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1219) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Knockout Validated

Western Blot Shows Human Integrin aV/CD51 Antibody Specificity by Using Knockout Cell Line.

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Western Blot Shows Human Integrin alpha V/CD51 Specificity by Using Knockout Cell Line. Western blot shows lysates of A549 human lung carcinoma parental cell line and Integrin aV/CD51 knockout A549 cell line (KO). PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human/Mouse/Rat Integrin aV/CD51 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1219) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Integrin aV/CD51 at approximately 120 kDa (as indicated) in the parental A549 cell line, but is not detectable in knockout A549 cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Integrin alpha V/CD51

Integrin alpha V, also known as CD51 and vitronectin receptor subunit alpha, is a 140-150 kDa integrin alpha chain that forms dimers with at least five beta chains including beta 1, 3, 5, 6, and 8. It is a 1018 amino acid (aa) residue type I membrane protein with a large (962 aa) extracellular domain (ECD) and a short (32 aa) cytoplasmic tail. The N-terminal region of alpha V, which is important for ligand binding, contains seven FG-GAP (phenylalanyl-glycyl and glycyl-alanyl-prolyl) concensus repeats that fold into a beta -propellar domain. Furin cleavage of the alpha V ECD occurs after Gly 889, generating a disulfide-linked, heteromeric subunit alpha V chain. alpha V-containing integrins bind multiple ECM molecules, including vitronectin, osteopontin, MMP-2, and TSP. The ECD of human Integrin alpha V shares 92% aa sequence identity with mouse Integrin alpha V ECD.

Entrez Gene IDs

3685 (Human); 16410 (Mouse); 296456 (Rat); 102116249 (Cynomolgus Monkey)

Alternate Names

antigen identified by monoclonal L230; CD51 antigen; CD51; Integrin alpha V; integrin alpha-V; integrin, alpha V (vitronectin receptor, alpha polypeptide, antigen CD51); ITGAV; MSK8; Vitronectin Receptor alpha; Vitronectin receptor subunit alpha; VNRA; VNRADKFZp686A08142

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Product Datasheet

COA

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Related Research Areas

Citations for Human/Mouse/Rat Integrin alpha V/CD51 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

7 Citations: Showing 1 - 7
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Fungal sensing by dectin-1 directs the non-pathogenic polarization of TH17 cells through balanced type I IFN responses in human DCs
Authors: SI Gringhuis, TM Kaptein, EBM Remmerswaa, A Drewniak, BA Wevers, B Theelen, GRAM D'Haens, T Boekhout, TBH Geijtenbee
Nature Immunology, 2022-12-01;0(0):.
Species: Human
Sample Types: Transfected Whole Cells
Applications: Flow Cytometry Control
Identification of distinct N-glycosylation patterns on extracellular vesicles from small-cell and non-small-cell lung cancer cells
Authors: K Kondo, Y Harada, M Nakano, T Suzuki, T Fukushige, K Hanzawa, H Yagi, K Takagi, K Mizuno, Y Miyamoto, N Taniguchi, K Kato, T Kanekura, N Dohmae, K Machida, I Maruyama, H Inoue
The Journal of Biological Chemistry, 2022-04-18;0(0):101950.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
Platelet-derived extracellular vesicles are increased in sera of Alzheimer's disease patients, as revealed by Tim4-based assays
Authors: H Odaka, K Hiemori, A Shimoda, K Akiyoshi, H Tateno
FEBS Open Bio, 2021-02-03;0(0):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
Combination therapy of cold atmospheric plasma (CAP) with temozolomide in the treatment of U87MG glioblastoma cells
Authors: E Gjika, S Pal-Ghosh, ME Kirschner, L Lin, JH Sherman, MA Stepp, M Keidar
Sci Rep, 2020-10-05;10(1):16495.
Species: Human
Sample Types: Whole Cells
Applications: ICC
Characterization of human and murine T-cell immunoglobulin mucin domain 4 (TIM-4) IgV domain residues critical for Ebola virus entry
J Virol, 2016-06-10;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
Quantity and accessibility for specific targeting of receptors in tumours.
Authors: Hussain, Sajid, Rodriguez-Fernandez, Maria, Braun, Gary B, Doyle, Francis, Ruoslahti, Erkki
Sci Rep, 2014-06-10;4(0):5232.
Species: Human
Sample Types: Whole Cells
Applications: IHC
Binding of extracellular maspin to beta1 integrins inhibits vascular smooth muscle cell migration.
Authors: Bass R, Wagstaff L, Ravenhill L, Ellis V
J. Biol. Chem., 2009-07-28;284(40):27712-20.
Species: Human
Sample Types: Cell Lysates
Applications: Immunoprecipitation, Western Blot