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Human/Mouse/Rat JNK1/JNK2 Antibody

Catalog #: MAB2076
5 Citations Datasheet / COA / SDS
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MAB2076
MAB2076-SP
Detection of Human/Mouse/Rat JNK1/JNK2 by Western Blot.
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Citations (5)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse/Rat JNK1/JNK2 Antibody Summary

Species Reactivity
Human, Mouse, Rat
Specificity
Detects human, mouse, and rat p46 and p54 JNK1/JNK2. Does not detect recombinant JNK3.
Source
Monoclonal Mouse IgG2A Clone # 252323
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human JNK1 isoform 2
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.2 µg/mL
See below
Simple Western
10 µg/mL
HEK293T human embryonic kidney cell line
Immunocytochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human/Mouse/Rat JNK1/JNK2 antibody by Western Blot. View Larger

Detection of Human/Mouse/Rat JNK1/JNK2 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and C2C12 mouse myoblast cell line. PVDF membrane was probed with 0.2 µg/mL Mouse Anti-Human/Mouse/Rat JNK1/JNK2 Monoclonal Antibody (Catalog # MAB2076) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). For additional reference, recombinant human JNK1, JNK2, and JNK3 (1 ng/lane) were included. Specific bands for JNK1 and JNK2 were detected at approximately 46 kDa and 54 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry JNK1/JNK2 antibody in HeLa Human Cervical Epithelial Carcinoma Cell Line by Immunocytochemistry (ICC). View Larger

JNK1/JNK2 in HeLa Human Cervical Epithelial Carcinoma Cell Line. JNK1/JNK2 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Mouse Anti-Human/Mouse/Rat JNK1/JNK2 Monoclonal Antibody (Catalog # MAB2076) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunocytochemistry View Larger

JNK1/JNK2 in C2C12 Mouse Cell Line. JNK1/JNK2 was detected in immersion fixed C2C12 mouse myoblast cell line using Mouse Anti-Human/Mouse/Rat JNK1/JNK2 Monoclonal Antibody (Catalog # MAB2076) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Simple Western View Larger

Detection of Human JNK1/JNK2 by Simple WesternTM. Simple Western lane view shows lysates of HEK293T human embryonic kidney cell line, loaded at 0.2 mg/mL. Specific bands were detected for JNK1/JNK2 at approximately 48 and 58 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human/Mouse/Rat JNK1/JNK2 Monoclonal Antibody (Catalog # MAB2076). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: JNK1/JNK2

The c-Jun N-terminal kinases (JNKs) are encoded by three genes: JNK1, JNK2, and JNK3. Members of the MAPK superfamily, JNKs are activated by environmental stresses and inflammatory cytokines. JNK1, also known as SAPK1 gamma and MAPK8, is expressed as four isoforms generated by alternative splicing. JNK1 is activated by dual phosphorylation at T183 and Y185 by the MAPK kinases MKK4 and/or MKK7.

Long Name
C-Jun N-terminal Kinase
Alternate Names
AI849689;AI851083;JNK;JNK1;JNK1A2;JNK2;JNK21B1/2;JNK2A;JNK2ALPHA;JNK2B;JNK2BETA;JNK-46;JNK-55;MAPK8;MAPK9;p54a;p54aSAPK;PRKM8;PRKM9;SAPK;SAPK1;SAPK1A;SAPK1C; JNK1/JNK2

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Citations for Human/Mouse/Rat JNK1/JNK2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. Activity of fibroblast-like synoviocytes in rheumatoid arthritis was impaired by dickkopf-1 targeting siRNA
    Authors: YY Liu, SY Wang, YN Li, WJ Bian, LQ Zhang, YH Li, L Long, X Liu, XW Zhang, ZG Li
    Chin. Med. J., 2020-03-20;0(6):679-686.
    Species: Human
    Sample Types: Cell Lysate
    Applications: Western Blot
  2. Ulinastatin Ameliorates Pulmonary Capillary Endothelial Permeability Induced by Sepsis Through Protection of Tight Junctions via Inhibition of TNF-? and Related Pathways
    Authors: M Fang, WH Zhong, WL Song, YY Deng, DM Yang, B Xiong, HK Zeng, HD Wang
    Front Pharmacol, 2018-08-13;9(0):823.
    Species: Rat
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  3. WWOX sensitises ovarian cancer cells to paclitaxel via modulation of the ER stress response
    Authors: S Janczar, J Nautiyal, Y Xiao, E Curry, M Sun, E Zanini, AJ Paige, H Gabra
    Cell Death Dis, 2017-07-27;8(7):e2955.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  4. High glucose alters retinal astrocytes phenotype through increased production of inflammatory cytokines and oxidative stress.
    Authors: Shin E, Huang Q, Gurel Z, Sorenson C, Sheibani N
    PLoS ONE, 2014-07-28;9(7):e103148.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  5. Pre-exposure of Mycobacterium tuberculosis-infected macrophages to crystalline silica impairs control of bacterial growth by deregulating the balance between apoptosis and necrosis.
    Authors: Chavez-Galan L, Ramon-Luing L, Torre-Bouscoulet L, Perez-Padilla R, Sada-Ovalle I
    PLoS ONE, 2013-11-22;8(11):e80971.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot

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