Human/Mouse/Rat Keap1 Antibody Summary
Ala90-Ile250
Accession # Q14145
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human/Mouse/Rat Keap1 by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line, Raji human Burkitt's lymphoma cell line, CH-1 mouse B cell lymphoma cell line, A20 mouse B cell lymphoma cell line, and Rat-2 rat embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat Keap1 Antigen Affinity‑purified Polyclonal Antibody (Catalog # AF3024) followed by HRP-conjugated Anti‑Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Keap1 at approximately 66 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 2.
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Western Blot Shows Human Keap1 Specificity by Using Knockout Cell Line. Western blot shows lysates of Jurkat human acute T cell leukemia parental cell line and Keap1 Jurkat cell line (KO). PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat Keap1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3024) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Keap1 at approximately 66 kDa (as indicated) in the parental Jurkat cell line, but is not detectable in knockout Jurkat cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Keap1
Kelch-like ECH-associated protein 1 (Keap1) is a 624 amino acid, 69 kDa protein which interacts with the transcription factor NF-E2-related factor 2 (Nrf2). Keap1 represses Nrf2 function by sequestering Nrf2 in the cytoplasm. Keap1 contains an N-terminal BTB domain and six C-terminal KELCH domains (aa 327‑611) that interact with Nrf2. Dissociation of the two proteins in response to redox-sensitive cell stress is followed by the translocation of Nrf2 to the nucleus and transcription of detoxifying/oxidative stress enzyme genes.
Product Datasheets
Citation for Human/Mouse/Rat Keap1 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
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Nrf2-inducing anti-oxidation stress response in the rat liver--new beneficial effect of lansoprazole.
Authors: Yamashita, Yasunobu, Ueyama, Takashi, Nishi, Toshio, Yamamoto, Yuta, Kawakoshi, Akatsuki, Sunami, Shogo, Iguchi, Mikitaka, Tamai, Hideyuki, Ueda, Kazuki, Ito, Takao, Tsuruo, Yoshihir, Ichinose, Masao
PLoS ONE, 2014-05-20;9(5):e97419.
Species: Rat
Sample Types: Tissue Homogenates
Applications: Western Blot
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