Human/Mouse/Rat MEK1 Antibody

Catalog #: MAB2079 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB2079
MAB2079-SP

Detection of Human/Mouse/Rat MEK1 by Western Blot.

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Human/Mouse/Rat MEK1 Antibody Summary

Species Reactivity

Human, Mouse, Rat

Specificity

Detects human, mouse, and rat MEK1 in Western blots. In Western blots, no crossreactivity with recombinant human MEK2 is observed.

Source

Monoclonal Mouse IgG_2A Clone # 239421

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human MEK1
Lys2-Val393 (Ser218Asp, Ser222Asp)
Accession # Q02750

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

0.5 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human/Mouse/Rat MEK1 by Western Blot. Western blot shows lysates of U937 human histiocytic lymphoma cell line, HepG2 human hepatocellular carcinoma cell line, DU145 human prostate carcinoma cell line, and TS1 mouse helper T cell line. PVDF membrane was probed with 0.5 µg/mL Mouse Anti-Human/ Mouse/Rat MEK1 Monoclonal Antibody (Catalog # MAB2079) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). For additional reference, recombinant human MEK1 and Recombinant Human Active MEK2 (Catalog # 2855-KS) (5 ng/lane) were included. A specific band for MEK1 was detected at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MEK1

Mitogen-Activated Protein Kinase Kinase 1 (MEK1 or MAP2K1), also known as MKK1, belongs to the family of STE kinases. Both MEK1 and the related MEK2 aredual‑specificity kinases, phosphorylating and activating the mitogen-activated protein kinases ERK1 and ERK2 at T and Y positions within the phosphoacceptor sequence T-E-Y. Activation of MEK1 by Raf occurs through phosphorylation at S218 and S222.

Long Name

Mitogen-activated Protein Kinase Kinase 1

Entrez Gene IDs

5604 (Human); 26395 (Mouse)

Alternate Names

dual specificity mitogen-activated protein kinase kinase 1; ERK activator kinase 1; MAP kinase kinase 1; MAP2K1; MAPK/ERK kinase 1; MAPKK1; MEK1; MEK1MAPKK 1; mitogen-activated protein kinase kinase 1; MKK1; MKK1MEK 1; PRKMK1EC 2.7.12.2; protein kinase, mitogen-activated, kinase 1 (MAP kinase kinase 1)