Home / Muscle Phosphofructokinase/PFKM / Human/Mouse/Rat Muscle Phosphofructokinase/PFKM Antibody

Human/Mouse/Rat Muscle Phosphofructokinase/PFKM Antibody

Catalog #: MAB7687
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MAB7687
MAB7687-SP
Detection of Human, Mouse, and Rat Muscle Phosphofructokinase/PFKM by Western Blot.
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Citations (1)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse/Rat Muscle Phosphofructokinase/PFKM Antibody Summary

Species Reactivity
Human, Mouse, Rat
Specificity
Detects human Muscle Phosphofructokinase/PFKM in direct ELISAs and Western blots. Detects mouse and rat Muscle Phosphofructokinase/PFKM in Western blots.
Source
Monoclonal Mouse IgG2B Clone # 842735
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human Muscle Phosphofructokinase/PFKM
Asn674-Val780
Accession # P08237
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated
Purity
Protein A or G purified from hybridoma culture supernatant

Applications

Recommended Concentration
Sample
Western Blot
0.2 µg/mL
See below
Simple Western
2 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human, Mouse, and Rat Muscle Phosphofructokinase/PFKM antibody by Western Blot. View Larger

Detection of Human, Mouse, and Rat Muscle Phosphofructokinase/PFKM by Western Blot. Western blot shows lysates of human heart tissue, mouse heart tissue, and rat heart tissue. PVDF membrane was probed with 0.2 µg/mL of Mouse Anti-Human Muscle Phospho-fructokinase/PFKM Monoclonal Antibody (Catalog # MAB7687) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Muscle Phosphofructokinase/PFKM at approximately 85 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western Detection of Human and Rat Muscle Phosphofructokinase/PFKM antibody by Simple Western<SUP>TM</SUP>. View Larger

Detection of Human and Rat Muscle Phosphofructokinase/PFKM by Simple WesternTM. Simple Western lane view shows lysates of human heart tissue and rat heart tissue, loaded at 0.5 mg/mL. A specific band was detected for Muscle Phosphofructokinase/PFKM at approximately 84 kDa (as indicated) using 2 µg/mL of Mouse Anti-Human/Mouse/Rat Muscle Phosphofructokinase/PFKM Monoclonal Antibody (Catalog # MAB7687). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Muscle Phosphofructokinase/PFKM

PFKM (PhosphoFructoKinase, Muscle type; also PFK-A and Phosphohexokinase)) is a 85-86 kDa member of the phosphofructokinase family, two domain subfamily of enzymes. It is one of three functionally related enzymes, all expressed by distinct genes. While PFKM is expressed by virtually all cells, its name is based on the fact that skeletal muscle expresses only the PFKM isotype. PFK catalyzes the essentially irreversible phosphorylation of Fru-6-P, forming Fru-1,6-bisP. This determines the rate of cellular glycolysis. As a monomer, PFKM is unstable, and following synthesis, PFKM forms low activity homodimers. High activity comes with tetramerization/oligomerization. In all cases, multimerization is associated with PFKM interaction with various components of the cell cytoskeleton. Human PFKM is 780 amino acids (aa) in length. It contains a 740 aa catalytic region (aa 16-755) plus one utilized phosphorylation site at Thr2. There are at least two potential isoform variants. One contains an alternative start site 71 aa upstream of the standard site, while another shows a deletion of aa 282-312. Proteolysis of PKFM apparently occurs, generating 45-47 kDa fragments. Over aa 674-780, human PFKM shares 98% aa sequence identity with mouse PFKM.

Long Name
Phosphofructokinase, Muscle
Entrez Gene IDs
5213 (Human); 18642 (Mouse); 65152 (Rat)
Alternate Names
6-phosphofructokinase, muscle type; EC 2.7.1,6-phosphofructo-1-kinase; EC 2.7.1.11; GSD7; MGC8699; Muscle Phosphofructokinase; PFK1; PFK-1; PFKA; PFK-A; PFKM; PFKX; Phosphofructo-1-kinase isozyme A; Phosphofructokinase 1; phosphofructokinase, muscle; phosphofructokinase, polypeptide X; Phosphofructokinase-1; Phosphofructokinase-M; Phosphohexokinase

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Citation for Human/Mouse/Rat Muscle Phosphofructokinase/PFKM Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. TAp73-induced phosphofructokinase-1 transcription promotes the Warburg effect and enhances cell proliferation
    Authors: L Li, L Li, W Li, T Chen, Bin Zou, L Zhao, H Wang, X Wang, L Xu, X Liu, D Wang, B Li, TW Mak, W Du, X Yang, P Jiang
    Nat Commun, 2018-11-08;9(1):4683.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot

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