Human/Mouse/Rat Nbs1 Antibody Summary
Pro498-Arg754
Accession # O60934
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human/Mouse/Rat Nbs1 by Western Blot. Western blot shows lysates of K562 human chronic myelogenous leukemia cell line, NIH-3T3 mouse embryonic fibroblast cell line, and NRK rat normal kidney cell line,. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat Nbs1 Monoclonal Antibody (Catalog # MAB1573) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Nbs1 at approximately 95 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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Western Blot Shows Human Nbs1 Specificity by Using Knockout Cell Line. Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and Nbs1 knockout HeLa cell line (KO). PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse/Rat Nbs1 Monoclonal Antibody (Catalog # MAB1573) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Nbs1 at approximately 95 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # MAB5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Nbs1
The Nijmegen Breakage Syndrome 1 (Nbs1) protein is a member of the Mre11/Rad50/Nbs1 (MRN) protein complex that binds to DNA double-strand breaks in cells exposed to DNA damaging agents. In addition, the MRN complex colocalizes with replication forks during DNA replication. The MRN complex plays an important role in routine cell cycle progression and genotoxic stress responses by facilitating DNA repair. In fact, mutation of the nbs1 gene and resultant loss of Nbs1 protein expression in humans results in the chromosomal instability disease, Nijmegen Breakage Syndrome.
Product Datasheets
Citation for Human/Mouse/Rat Nbs1 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
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NBS1 recruits RAD18 via a RAD6-like domain and regulates Pol eta-dependent translesion DNA synthesis.
Authors: Yanagihara H, Kobayashi J, Tateishi S, Kato A, Matsuura S, Tauchi H, Yamada K, Takezawa J, Sugasawa K, Masutani C, Hanaoka F, Weemaes CM, Mori T, Zou L, Komatsu K
Mol. Cell, 2011-09-02;43(5):788-97.
Species: Human
Sample Types: Cell Lysates
Applications: Immunoprecipitation
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