Human/Mouse/Rat Ninjurin-2 Antibody

Catalog #: AF5056 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF5056
AF5056-SP

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Human/Mouse/Rat Ninjurin-2 Antibody Summary

Species Reactivity

Human, Mouse, Rat

Specificity

Detects human, mouse, and rat Ninjurin-2 in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant human Ninjurin-1 is observed.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human Ninjurin-2 Met1-Thr65 Accession # Q9NZG7

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Human thymus, mouse thymus, rat thymus, Ntera-2, HT-29

Immunohistochemistry

3-15 µg/mL

Immersion fixed paraffin-embedded sections of Human Tonsil

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry

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Detection of Ninjurin‑2 in Human Tonsil Ninjurin‑2 was detected in immersion fixed paraffin-embedded sections of Human Tonsil using Sheep Anti-Human/Mouse/Rat Ninjurin‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5056) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Sheep IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC006). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell surface on lymphocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Western Blot

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Detection of Human/Mouse/Rat Ninjurin‑2 by Western Blot. Western blot shows lysates of human thymus, mouse thymus, rat thymus, NTera‑2 human testicular embryonic carcinoma cells and HT‑29 human colon adenocarcinoma cells. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human/Mouse/Rat Ninjurin‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5056) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Ninjurin‑2 at approximately ~26kDa kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

Background: Ninjurin-2

Ninjurin-2 (nerve injury-induced protein 2) is a 20-22 kDa member of the Ninjurin family of transmembrane (TM) proteins. It is expressed by multiple cell types, including Schwann cells, myenteric plexus and sensory neurons, and lymphocytes and participates in intercellular homophilic binding. Human Ninjurin-2 is 142 amino acids (aa) in length. It has an unusual membrane orientation. There is a 65 aa N-terminal extracellular domain (ECD) (aa 1-65) that contains one phoshorylation site at Ser3, followed by a TM segment, a cytoplasmic region, a second TM segment and a C-terminal ECD (aa 128-142). One potential alternate start site exists 46 aa upstream of the standard form start site. Over aa 1-65, human Ninjurin-2 is 71% aa identical to mouse Ninjurin-2.

Entrez Gene IDs

4815 (Human); 29862 (Mouse); 59115 (Rat)

Alternate Names

FLJ56349; Nerve injury-induced protein 2; NINJ2; ninjurin 2; Ninjurin2; Ninjurin-2