Home / NKX2.2 / Human/Mouse/Rat NKX2.2 Antibody

Human/Mouse/Rat NKX2.2 Antibody

Catalog #: MAB8167
2 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB8167
MAB8167-SP
Detection of Human and Mouse NKX2.2 by Western Blot.
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Product Details
Citations (2)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse/Rat NKX2.2 Antibody Summary

Species Reactivity
Human, Mouse, Rat
Specificity
Detects human NKX2.2 in ELISA and Western Blot.
Source
Monoclonal Mouse IgG2B Clone # 883411
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human NKX2.2
Met1-Lys128
Accession # O95096
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Purity
Protein A or G purified from hybridoma culture supernatant

Applications

Recommended Concentration
Sample
Western Blot
2 µg/mL
See below
Immunocytochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human and Mouse NKX2.2 antibody by Western Blot. View Larger

Detection of Human and Mouse NKX2.2 by Western Blot. Western blot shows lysates of A-673 human Ewing's sarcoma cell line and beta TC-6 mouse beta cell insulinoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human/Mouse/Rat NKX2.2 Monoclonal Antibody (Catalog # MAB8167) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for NKX2.2 at approximately 35 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry NKX2 by Immunocytochemistry (ICC).2 antibody in A673 Human Cell Line by Immunocytochemistry (ICC). View Larger

NKX2.2 in A673 Human Cell Line. NKX2.2 was detected in immersion fixed A673 human Ewing sarcoma cell line using Mouse Anti-Human/Mouse/Rat NKX2.2 Monoclonal Antibody (Catalog # MAB8167) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunocytochemistry NKX2 by Immunocytochemistry (ICC).2 antibody in Rat Cortical Stem Cells by Immunocytochemistry (ICC). View Larger

NKX2.2 in Rat Cortical Stem Cells. NKX2.2 was detected in immersion fixed rat cortical stem cells differentiated for 7 days using Mouse Anti-Human/Mouse/Rat NKX2.2 Monoclonal Antibody (Catalog # MAB8167) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: NKX2.2

NKX2.2, also known as NKX2-2 or NKX2B, is an approximately 30 kDa, 273 amino acid homeobox nuclear transcription factor of the NKX2 protein family. It specifies differentiation of pancreatic islet cells such as alpha, beta, PP, and epsilon lineages.  It also functions in developmental neuronal patterning of the ventral spinal cord in response to graded Sonic Hedgehog gradients and contributes pathfinding of commissural neurons. Within the region used as an immunogen, human NKX2.2 shares 96% and 97% amino acid sequence identity with mouse and rat NKX2.2, respectively.

Long Name
NK2 Homeobox 2
Entrez Gene IDs
4821 (Human); 18088 (Mouse); 366214 (Rat)
Alternate Names
Homeobox protein NK-2 homolog B; NK-2 (Drosophila) homolog B; NK2 homeobox 2; NK2 transcription factor related, locus 2 (Drosophila); NK2 transcription factor related, locus 2; NK2 transcription factor-like protein B; NKX2.2; NKX2.2homeobox protein Nkx-2.2; NKX2B; NKX2BNK-2 homolog B

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Citations for Human/Mouse/Rat NKX2.2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. C21orf91 Regulates Oligodendroglial Precursor Cell Fate-A Switch in the Glial Lineage?
    Authors: L Reiche, P Göttle, L Lane, P Duek, M Park, K Azim, J Schütte, A Manousi, J Schira-Hei, P Küry
    Frontiers in Cellular Neuroscience, 2021-03-16;15(0):653075.
    Species: Rat
    Sample Types: Whole Cells
    Applications: ICC
  2. Integrator of Stress Responses Calmodulin Binding Transcription Activator 1 (Camta1) Regulates miR-212/miR-132 Expression and Insulin Secretion
    J Biol Chem, 2016-07-08;0(0):.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay

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