Human/Mouse/Rat p38 gamma Antibody

Catalog #: AF1347
7 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF1347
AF1347-SP

Detection of Mouse/Rat p38 gamma by Western Blot.

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All p38 gamma Antibodies

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Citations (7)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse/Rat p38 gamma Antibody Summary

Species Reactivity

Human, Mouse, Rat

Specificity

Detects human, mouse, and rat p38 gamma in Western blots. The antibody is highly specific for p38 gamma and in Western blots, shows extremely weak cross-reactivity with recombinant p38 alpha, p38 beta, or p38δ after prolonged exposure to film.

Source

Polyclonal Rabbit IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human p38 gamma
Accession # P53778

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.2 µg/mL

See below

Simple Western

2 µg/mL

See below

Immunohistochemistry

5-15 µg/mL

Immersion fixed paraffin-embedded sections of human breast, human skin cancer tissue, and mouse skeletal muscle

Immunocytochemistry

3-15 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Mouse/Rat p38 gamma by Western Blot. Western blot shows lysates of PC-12 rat adrenal pheochromocytoma cell line, C6 rat glioma cell line, and C2C12 mouse myoblast cell line. PVDF membrane was probed with 0.2 µg/mL Rabbit Anti-Human/Mouse/Rat p38 gamma Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1347) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band for p38 gamma was detected at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry

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p38 gamma in MCF‑7 Human Cell Line. p38 gamma was detected in immersion fixed MCF-7 human breast cancer cell line using Rabbit Anti-Human/Mouse/Rat p38 gamma Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1347) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Western Blot

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Detection of human p38 gamma by Western Blot. Western blot shows recombinant human p38 beta, p38 gamma, p38d and p38a (1 ng/lane). PVDF membrane was probed with 0.2 µg/mL Rabbit Anti-Human/Mouse/Rat p38 gamma Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1347) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band for p38 gamma was detected at approx-imately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western

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Detection of Mouse and Rat p38 gamma by Simple Western^TM. Simple Western lane view shows lysates of C2C12 mouse myoblast cell line and PC‑12 rat adrenal pheochromocytoma cell line, loaded at 0.2 mg/mL. A specific band was detected for p38 gamma at approximately 48 kDa (as indicated) using 2 µg/mL of Rabbit Anti-Human/Mouse/Rat p38 gamma Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1347). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: p38 gamma

p38 mitogen-activated protein kinase (MAPK)gamma, also known as p38 gamma, MAPK12, SAPK3 and ERK6 is a serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. Plays a role in myoblast differentiation and also in the
down-regulation of cyclin D1 in response to hypoxia in adrenal cells.

Entrez Gene IDs

6300 (Human)

Alternate Names

EC 2.7.11; ERK3; ERK-6; ERK6MAPK 12; Extracellular signal-regulated kinase 6; MAP kinase 12; MAP kinase p38 gamma; MAPK12; mitogen-activated protein kinase 12; mitogen-activated protein kinase 3; Mitogen-activated protein kinase p38 gamma; p38 gamma; p38gamma; PRKM12; SAPK-3; SAPK3EC 2.7.11.24; Stress-activated protein kinase 3

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human/Mouse/Rat p38 gamma Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

7 Citations: Showing 1 - 7
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Treatment of epilepsy using a targeted p38gamma kinase gene therapy
Authors: N Morey, M Przybyla, J van der Ho, YD Ke, F Delerue, J van Eersel, LM Ittner
Science Advances, 2022-12-02;8(48):eadd2577.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot
Role of Alterations in Protein Kinase p38&gamma in the Pathogenesis of the Synaptic Pathology in Dementia With Lewy Bodies and &alpha-Synuclein Transgenic Models
Authors: M Iba, C Kim, J Florio, M Mante, A Adame, E Rockenstei, S Kwon, R Rissman, E Masliah
Front Neurosci, 2020-03-31;14(0):286.
Species: Human, Mouse
Sample Types: Whole Tissue
Applications: IHC
Impact of p38? Mitogen-Activated Protein Kinase (MAPK) on MDA-MB-231 Breast Cancer Cells Using Metabolomic Approach
Authors: H Chen, X Wang, F Guo, P Li, D Peng, J He
Int. J. Biochem. Cell Biol., 2018-11-14;0(0):.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P
Potential Neuroprotective Effects of an LSD1 Inhibitor in Retinal Ganglion Cells via p38 MAPK Activity
Invest. Ophthalmol. Vis. Sci, 2016-11-01;57(14):6461-6473.
Species: Rat
Sample Types: Whole Cells
Applications: IHC-Fr
Kif13b Regulates PNS and CNS Myelination through the Dlg1 Scaffold
Authors: R Noseda, M Guerrero-V, V Alberizzi, SC Previtali, DL Sherman, M Palmisano, RL Huganir, KA Nave, A Cuenda, ML Feltri, PJ Brophy, A Bolino
PLoS Biol, 2016-04-12;14(4):e1002440.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot
p38gamma MAPK Is a Therapeutic Target for Triple-Negative Breast Cancer by Stimulation of Cancer Stem-Like Cell Expansion.
Authors: Qi X, Yin N, Ma S, Lepp A, Tang J, Jing W, Johnson B, Dwinell M, Chitambar C, Chen G
Stem Cells, 2015-06-23;33(9):2738-47.
Species: Human, Mouse
Sample Types: Cell Lysates, Whole Tissue
Applications: IHC, Western Blot
Differential tissue expression and activation of p38 MAPK alpha, beta, gamma, and delta isoforms in rheumatoid arthritis.
Authors: Korb A, Tohidast-Akrad M, Cetin E, Axmann R, Smolen J, Schett G
Arthritis Rheum., 2006-09-01;54(9):2745-56.
Species: Human
Sample Types: Tissue Homogenates
Applications: IHC, Immunoprecipitation, Western Blot