Human/Mouse/Rat PAK4 Antibody

Catalog #: AF4178 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF4178
AF4178-SP

Detection of Human/Mouse/Rat PAK4 by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse/Rat PAK4 Antibody Summary

Species Reactivity

Human, Mouse, Rat

Specificity

Detects endogenous human, mouse and rat PAK4 in Western blots.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human PAK4
Met1-Glu120
Accession # O96013

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Purity

Immunogen affinity purified

Applications

Recommended Concentration

Sample

Western Blot

2 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human/Mouse/Rat PAK4 by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line, MCF-7 human breast cancer cell line, PT18 mouse mast/basophil cell line, and NIH-3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 2 µg/mL of Human/Mouse/Rat PAK4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4178) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for PAK4 at approximately 72 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PAK4

As downstream targets of the Rho GTPases, the p21-activated kinase (PAK) family of serine/threonine kinases regulates the organization of the actin cytoskeleton in mammalian cells. The PAK family is structurally categorized in two groups, each with three members: group I PAK1-3; and group II PAK4-6. Targeted disruption of PAK4 in mice leads to severe defects in the development and migration of neurons, suggesting a key role for this kinase in neuronal development.

Long Name

p21/Cdc42/Rac1-activated Kinase 4

Entrez Gene IDs

10298 (Human); 70584 (Mouse); 292756 (Rat)

Alternate Names

EC 2.7.11; EC 2.7.11.1; KIAA1142; p21 protein (Cdc42/Rac)-activated kinase 4; p21(CDKN1A)-activated kinase 4; p21-activated kinase 4; PAK4; PAK-4; protein kinase related to S. cerevisiae STE20, effector for Cdc42Hs; serine/threonine-protein kinase PAK 4