Home / ATM / Human/Mouse/Rat Phospho-ATM (S1981) Antibody

Human/Mouse/Rat Phospho-ATM (S1981) Antibody

Catalog #: AF1655
13 Citations Datasheet / COA / SDS
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AF1655
AF1655-SP
Detection of Human/Mouse/Rat Phospho-ATM (S1981) by Western Blot.
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Citations (13)
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Human/Mouse/Rat Phospho-ATM (S1981) Antibody Summary

Species Reactivity
Human, Mouse, Rat
Specificity
Detects human ATM when phosphorylated at S1981. Also detects the comparable phosphorylated sites in mouse ATM (S1987) and rat ATM (S1952).
Source
Polyclonal Rabbit IgG
Purification
Antigen Affinity-purified
Immunogen
Phosphopeptide containing human ATM S1981 site
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Purity
Immunogen affinity purified

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Simple Western
10 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human/Mouse/Rat Phospho-ATM (S1981) antibody by Western Blot. View Larger

Detection of Human/Mouse/Rat Phospho-ATM (S1981) by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line untreated (-) or treated (+) with 1 µM camptothecin (CPT) for 1 hour. PVDF membrane was probed with 1 µg/mL Rabbit Anti-Human/Mouse/Rat Phospho-ATM (S1981) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1655) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band for Phospho-ATM (S1981) was detected at approximately 370 kDa (as indicated). The phospho-specificity of this antibody was supported by decreased labeling following treatment with 600 U lambda-phosphatase (lambda-PPase) for 1 hour. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western Detection of Human Phospho-ATM (S1981) antibody by Simple Western<SUP>TM</SUP>. View Larger

Detection of Human Phospho-ATM (S1981) by Simple WesternTM. Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cell line untreated (-) or treated (+) with 1 µM Camptothecin (CPT) for 1 hour, loaded at 0.2 mg/mL. A specific band was detected for Phospho-ATM (S1981) at approximately 270 kDa (as indicated) using 10 µg/mL of Rabbit Anti-Human/Mouse/Rat Phospho-ATM (S1981) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1655). This experiment was conducted under reducing conditions and using the 66-440 kDa separation system.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ATM

The Ataxia telangiectasia-mutated (ATM) protein kinase exists as a dimer in the cell nucleus. Changes in DNA structure induced by genotoxic stress lead to activation of ATM and phosphorylation of S1981 in trans. Once S1981 is phosphorylated, the dimer dissociates and active ATM monomers signal to downstream targets.

Long Name
Ataxia Telangiectasia-mutated
Entrez Gene IDs
472 (Human); 11920 (Mouse)
Alternate Names
AT mutated; A-T mutated; AT1; ATA; ataxia telangiectasia mutated (includes complementation groups A, C and D); ataxia telangiectasia mutated; ATC; ATD; ATDC; ATE; ATM serine/threonine kinase; ATM; DKFZp781A0353; EC 2.7.11.1; MGC74674; serine-protein kinase ATM; TEL1; TEL1, telomere maintenance 1, homolog; TELO1; TPLL

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Citations for Human/Mouse/Rat Phospho-ATM (S1981) Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

13 Citations: Showing 1 - 10
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  1. Replication Stress Response Modifies Sarcomeric Cardiomyopathy Remodeling
    Authors: S Pal, BR Nixon, MS Glennon, P Shridhar, SL Satterfiel, YR Su, JR Becker
    Journal of the American Heart Association, 2021-07-29;10(15):e021768.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  2. ATAD5 promotes replication restart by regulating RAD51 and PCNA in response to replication stress
    Authors: SH Park, N Kang, E Song, M Wie, EA Lee, S Hwang, D Lee, JS Ra, IB Park, J Park, S Kang, JH Park, S Hohng, KY Lee, K Myung
    Nat Commun, 2019-12-16;10(1):5718.
    Species: Human
    Sample Types: Whole Cells
    Applications: Western Blot
  3. ATM directs DNA damage responses and proteostasis via genetically separable pathways.
    Authors: Ji-Hoon Lee, Michael R Mand, Chung-Hsuan Kao, Yi Zhou, Seung W Ryu, Alicia L Richards, Joshua J Coon, Tanya T Paull
    Science Signaling, 2018-01-09;0(0):1937-9145.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  4. DEK is required for homologous recombination repair of DNA breaks
    Authors: EA Smith, B Gole, NA Willis, R Soria, LM Starnes, EF Krumpelbec, AG Jegga, AM Ali, H Guo, AR Meetei, PR Andreassen, F Kappes, LM Vinnedge, JA Daniel, R Scully, L Wiesmüller, SI Wells
    Sci Rep, 2017-03-20;7(0):44662.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  5. PRMT5-Dependent Methylation of the TIP60 Coactivator RUVBL1 Is a Key Regulator of Homologous Recombination
    Authors: TL Clarke, MP Sanchez-Ba, K Chiang, JJ Reynolds, J Herrero-Ru, TM Bandeiras, PM Matias, SL Maslen, JM Skehel, GS Stewart, CC Davies
    Mol. Cell, 2017-02-23;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  6. DNA-PK-A candidate driver of hepatocarcinogenesis and tissue biomarker that predicts response to treatment and survival.
    Authors: Cornell L, Munck J, Alsinet C, Villanueva A, Ogle L, Willoughby C, Televantou D, Thomas H, Jackson J, Burt A, Newell D, Rose J, Manas D, Shapiro G, Curtin N, Reeves H
    Clin Cancer Res, 2014-12-05;21(4):925-33.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  7. Chronic inflammation induces telomere dysfunction and accelerates ageing in mice.
    Authors: Jurk D, Wilson C, Passos J, Oakley F, Correia-Melo C, Greaves L, Saretzki G, Fox C, Lawless C, Anderson R, Hewitt G, Pender S, Fullard N, Nelson G, Mann J, van de Sluis B, Mann D, von Zglinicki T
    Nat Commun, 2014-06-24;2(0):4172.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  8. Direct activation of ATM by resveratrol under oxidizing conditions.
    Authors: Lee, Ji-Hoon, Guo, Zhi, Myler, Logan R, Zheng, Suting, Paull, Tanya T
    PLoS ONE, 2014-06-16;9(6):e97969.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  9. Severe reaction to radiotherapy for breast cancer as the presenting feature of ataxia telangiectasia.
    Authors: Byrd PJ, Srinivasan V, Last JI
    Br. J. Cancer, 2011-12-06;106(2):262-8.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  10. Lymphoid tumours and breast cancer in ataxia telangiectasia; substantial protective effect of residual ATM kinase activity against childhood tumours.
    Authors: Reiman A, Srinivasan V, Barone G
    Br. J. Cancer, 2011-07-26;105(4):586-91.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  11. RAD51D protects against MLH1-dependent cytotoxic responses to O(6)-methylguanine.
    Authors: Rajesh P, Rajesh C, Wyatt MD
    DNA Repair (Amst.), 2010-02-04;9(4):458-67.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  12. Inhibition of CHK1 kinase by Go6976 converts 8-chloro-adenosine-induced G2/M arrest into S arrest in human myelocytic leukemia K562 cells.
    Authors: Jia XZ, Yang SY, Zhou J, Li SY, Ni JH, An GS, Jia HT
    Biochem. Pharmacol., 2008-11-18;77(5):770-80.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  13. Sonic Hedgehog signaling impairs ionizing radiation-induced checkpoint activation and induces genomic instability.
    Authors: Leonard JM, Ye H, Wetmore C, Karnitz LM
    J. Cell Biol., 2008-10-27;183(3):385-91.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot

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