Human/Mouse/Rat Phospho-ERK1(T202/Y204)/ERK2 (T185/Y187) Antibody Summary
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human Phospho-ERK1 (T202/Y204) and ERK2 (T185/Y187) by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line untreated (-) or treated (+) with 200 nM PMA for for the indicated times. PVDF membrane was probed with 0.1 µg/mL of Rabbit Anti-Human/Mouse/Rat Phospho-ERK1 (T202/Y204)/ERK2 (T185/Y187) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1018), followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). Specific bands were detected for Phospho-ERK1 (T202/Y204) and ERK2 (T185/Y187) at approximately 42 and 44 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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Detection of Phospho-ERK1/ERK2 in Jurkat Human Cell Line by Flow Cytometry. Jurkat human acute T cell leukemia cell line were untreated (yellow line open histogram) or treated with 200 ng/mL PMA for 15 minutes (filled histogram) and stained with Rabbit Anti-Human/Mouse/Rat Phospho-ERK1/ERK2 (ERK1 T202/Y204, ERK2 T185/Y187) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1018) or control antibody (Catalog # AB-105-C, blue line open histogram), followed by Fluorescein-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0112). To facilitate intracellular staining, cells were fixed with PFA and permeabilized with ice-cold methanol.
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ERK1/ERK2 in Rat Brain. ERK1/ERK2 was detected in perfusion fixed frozen sections of rat brain (cortex) using 15 µg/mL Rabbit Anti-Human/Mouse/Rat Phospho-ERK1/ERK2 (ERK1 T202/Y204, ERK2 T185/Y187) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1018) overnight at 4 °C. Tissue was stained with the Anti-Rabbit HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS005) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
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Detection of Human Phospho-ERK1 (T202/Y204)/ERK2 (T185/Y187) by Simple WesternTM. Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cell line untreated (-) or treated (+) with PMA, loaded at 0.2 mg/mL. A specific band was detected for ERK1 (T202/Y204)/ERK2 (T185/Y187) at approximately 44 kDa (as indicated) using 5 µg/mL of Rabbit Anti-Human/Mouse/Rat Phospho-ERK1 (T202/Y204)/ERK2 (T185/Y187) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1018). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: ERK1/ERK2
ERK1 is a protein Serine/Threonine kinase that is a member of the extracellular signal-regulated kinases (ERKs) which are activated in response to numerous growth factors and cytokines (1). Activation of ERK1 requires both tyrosine and threonine phosphorylation that is mediated by MEK. ERK1 is ubiquitously distributed in tissues with the highest expression in heart, brain, and spinal cord. Activated ERK1 translocates into the nucleus where it phosphorylates various transcription factors.
Product Datasheets
Citations for Human/Mouse/Rat Phospho-ERK1(T202/Y204)/ERK2 (T185/Y187) Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 10
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Neuropharmacological Evidence Implicating Drug-Induced Glutamate Receptor Dysfunction in Affective and Cognitive Sequelae of Subchronic Methamphetamine Self-Administration in Mice
Authors: Denning, CJE;Madory, LE;Herbert, JN;Cabrera, RA;Szumlinski, KK;
International journal of molecular sciences
Species: Murine polyomavirus strain A3
Sample Types: Tissue Homogenates
Applications: Western Blot -
Dose-Dependent Biphasic Action of Quetiapine on AMPK Signalling via 5-HT7 Receptor: Exploring Pathophysiology of Clinical and Adverse Effects of Quetiapine
Authors: M Okada, K Fukuyama, E Motomura
International Journal of Molecular Sciences, 2022-08-14;23(16):.
Species: Rat
Sample Types: Cell Lysates
Applications: Simple Western -
Brexpiprazole Reduces 5-HT7 Receptor Function on Astroglial Transmission Systems
Authors: K Fukuyama, E Motomura, M Okada
International Journal of Molecular Sciences, 2022-06-12;23(12):.
Species: Rat
Sample Types: Cell Lysates
Applications: Western Blot -
Distinct Effects of Escitalopram and Vortioxetine on Astroglial L-Glutamate Release Associated with Connexin43
Authors: T Shiroyama, K Fukuyama, M Okada
International Journal of Molecular Sciences, 2021-09-16;22(18):.
Species: Rat
Sample Types: Cell Lysates
Applications: Capillary Immunoblot -
Different properties of mammary carcinogenesis induced by two chemical carcinogens, DMBA and PhIP, in heterozygous BALB/c Trp53 knockout mice
Authors: Y Machida, T Imai
Oncology Letters, 2021-08-16;22(4):738.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Regulation of CREB Phosphorylation in Nucleus Accumbens after Relief Conditioning
Authors: E Soleimanpo, JR Bergado Ac, P Landgraf, D Mayer, E Dankert, DC Dieterich, M Fendt
Cells, 2021-01-26;10(2):.
Species: Rat
Sample Types: Tissue Homogenates
Applications: Western Blot -
IL-24 deficiency protects mice against bleomycin-induced pulmonary fibrosis by repressing IL-4-induced M2 program in macrophages
Authors: LZ Rao, Y Wang, L Zhang, G Wu, L Zhang, FX Wang, LM Chen, F Sun, S Jia, S Zhang, Q Yu, JH Wei, HR Lei, T Yuan, J Li, X Huang, B Cheng, J Zhao, Y Xu, BW Mo, CY Wang, H Zhang
Cell Death Differ, 2020-11-03;0(0):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Increased Release of Proinflammatory Proteins in Primary Human Adipocytes and Activation of the Inflammatory NF?B, p38, and ERK Pathways upon Omentin Treatment
Authors: C Niersmann, K Röhrig, M Blüher, M Roden, C Herder, M Carstensen
Obes Facts, 2020-04-06;13(2):221-236.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Fluid supplementation accelerates epithelial repair during chemical colitis
Authors: JF Burgueño, JK Lang, AM Santander, I Fernández, E Fernández, J Zaias, MT Abreu
PLoS ONE, 2019-04-19;14(4):e0215387.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-P -
Characterization of TRKA signaling in acute myeloid leukemia
Authors: SM Herbrich, S Kannan, RM Nolo, M Hornbaker, J Chandra, PA Zweidler-M
Oncotarget, 2018-07-10;9(53):30092-30105.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
PI(4,5)P2 determines the threshold of mechanical force-induced B cell activation
Authors: Z Wan, C Xu, X Chen, H Xie, Z Li, J Wang, X Ji, H Chen, Q Ji, S Shaheen, Y Xu, F Wang, Z Tang, JS Zheng, W Chen, J Lou, W Liu
J. Cell Biol., 2018-04-23;0(0):.
Species: Mouse
Sample Types: Whole Cells
Applications: ICC -
Ferric citrate and ferric EDTA but not ferrous sulfate drive amphiregulin-mediated activation of the MAP kinase ERK in gut epithelial cancer cells
Authors: NM Scheers, DIA Pereira, N Faria, JJ Powell
Oncotarget, 2018-03-30;9(24):17066-17077.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Glycans modify mesenchymal stem cell differentiation to impact the function of resulting osteoblasts
Authors: KM Wilson, AM Jagger, M Walker, E Seinkmane, JM Fox, R Kröger, P Genever, D Ungar
J. Cell. Sci., 2018-02-14;0(0):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Obesity Increases Mitogen-Activated Protein Kinase Phosphatase-3 Levels in the Hypothalamus of Mice
Authors: BA Rodrigues, VR Muñoz, GK Kuga, RC Gaspar, SCBR Nakandakar, BM Crisol, JD Botezelli, LSS Pauli, ASR da Silva, LP de Moura, DE Cintra, ER Ropelle, JR Pauli
Front Cell Neurosci, 2017-10-09;11(0):313.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot -
Use of non-selective ?-blockers is associated with decreased tumor proliferative indices in early stage breast cancer
Authors: A Montoya, CN Amaya, A Belmont, N Diab, R Trevino, G Villanueva, S Rains, LA Sanchez, N Badri, S Otoukesh, A Khammanivo, D Liss, ST Baca, RJ Aguilera, EB Dickerson, A Torabi, AK Dwivedi, A Abbas, K Chambers, BA Bryan, Z Nahleh
Oncotarget, 2017-01-24;8(4):6446-6460.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Sirtuin 3 (SIRT3) Regulates ?-Smooth Muscle Actin (?-SMA) Production through the Succinate Dehydrogenase-G Protein-coupled Receptor 91 (GPR91) Pathway in Hepatic Stellate Cells
J Biol Chem, 2016-02-24;291(19):10277-92.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Mycobacteria bypass mucosal NF-kB signalling to induce an epithelial anti-inflammatory IL-22 and IL-10 response.
Authors: Lutay N, Hakansson G, Alaridah N, Hallgren O, Westergren-Thorsson G, Godaly G
PLoS ONE, 2014-01-28;9(1):e86466.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Ability of the Met kinase inhibitor crizotinib and new generation EGFR inhibitors to overcome resistance to EGFR inhibitors.
Authors: Nanjo, Shigeki, Yamada, Tadaaki, Nishihara, Hiroshi, Takeuchi, Shinji, Sano, Takako, Nakagawa, Takayuki, Ishikawa, Daisuke, Zhao, Lu, Ebi, Hiromich, Yasumoto, Kazuo, Matsumoto, Kunio, Yano, Seiji
PLoS ONE, 2013-12-26;8(12):e84700.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Influence of gefitinib and erlotinib on apoptosis and c-MYC expression in H23 lung cancer cells.
Authors: Suenaga M, Yamamoto M, Tabata S, Itakura S, Miyata M, Hamasaki S, Furukawa T
Anticancer Res, 2013-04-01;33(4):1547-54.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Perlecan Domain V induces VEGF secretion in brain endothelial cells through integrin alpha5beta1 and ERK-dependent signaling pathways.
Authors: Clarke D, Al Ahmad A, Lee B, Parham C, Auckland L, Fertala A, Kahle M, Shaw C, Roberts J, Bix G
PLoS ONE, 2012-09-17;7(9):e45257.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
The EGFR ligands amphiregulin and heparin-binding egf-like growth factor promote peritoneal carcinomatosis in CXCR4-expressing gastric cancer.
Authors: Yasumoto K, Yamada T, Kawashima A, Wang W, Li Q, Donev IS, Tacheuchi S, Mouri H, Yamashita K, Ohtsubo K, Yano S
Clin. Cancer Res., 2011-04-11;17(11):3619-30.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Transient PI3K Inhibition Induces Apoptosis and Overcomes HGF-Mediated Resistance to EGFR-TKIs in EGFR Mutant Lung Cancer.
Authors: Donev IS, Wang W, Yamada T, Li Q, Takeuchi S, Matsumoto K, Yamori T, Nishioka Y, Sone S, Yano S
Clin. Cancer Res., 2011-01-10;17(8):2260-9.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Deoxygedunin, a natural product with potent neurotrophic activity in mice.
Authors: Jang SW, Liu X, Chan CB
PLoS ONE, 2010-07-13;5(7):e11528.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
Long-term high glucose concentration influences Akt, ERK1/2, and PTP1B protein expression in human aortic smooth muscle cells.
Authors: Popov D, Nemecz M, Dumitrescu M, Georgescu A, Bohmer FD
Biochem. Biophys. Res. Commun., 2009-08-04;388(1):51-5.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
CB(2) cannabinoid receptor activation is cardioprotective in a mouse model of ischemia/reperfusion.
Authors: Montecucco F, Lenglet S, Braunersreuther V, Burger F, Pelli G, Bertolotto M, Mach F, Steffens S
J. Mol. Cell. Cardiol., 2009-01-07;46(5):612-20.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot -
E-selectin regulates gene expression in metastatic colorectal carcinoma cells and enhances HMGB1 release.
Authors: Aychek T, Miller K, Sagi-Assif O, Levy-Nissenbaum O, Israeli-Amit M, Pasmanik-Chor M, Jacob-Hirsch J, Amariglio N, Rechavi G, Witz IP
Int. J. Cancer, 2008-10-15;123(8):1741-50.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
The RAS/Raf1/MEK/ERK signaling pathway facilitates VSV-mediated oncolysis: implication for the defective interferon response in cancer cells.
Authors: Noser JA, Mael AA, Sakuma R, Ohmine S, Marcato P, Lee PW, Ikeda Y
Mol. Ther., 2007-05-15;15(8):1531-6.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
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