Human/Mouse/Rat Phospho-MEK1 (S218/S222)/MEK2 (S222/S226) Antibody

Catalog #: MAB8407 Datasheet / COA / SDS

Recombinant Monoclonal Antibody

Catalog #	Availability	Size / Price	Qty
MAB8407
MAB8407-SP

Detection of Human Phospho-MEK1 (S218/S222)/ MEK2 (S222/S226) by Western Blot.

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Human/Mouse/Rat Phospho-MEK1 (S218/S222)/MEK2 (S222/S226) Antibody Summary

Species Reactivity

Human, Mouse, Rat

Specificity

Detects human, mouse, and rat MEK1 and MEK2 dually phosphorylated at S218/S222 and S222/S226, respectively.

Source

Recombinant Monoclonal Rabbit IgG Clone # 1020E

Purification

Protein A or G purified from cell culture supernatant

Immunogen

Phosphopeptide containing the human, mouse, and rat MEK1 S218/S222 site

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.5 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human Phospho-MEK1 (S218/S222)/ MEK2 (S222/S226) by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line and HeLa human cervical epithelial carcinoma cell line untreated (-) or treated (+) with 200 nM PMA or PMA and Ionomycin for 20 minutes. PVDF membrane was probed with 0.5 µg/mL of Rabbit Anti-Human/Mouse/Rat Phospho-MEK1 (S218/S222)/ MEK2 (S222/S226) Monoclonal Antibody (Catalog # MAB8407) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-MEK1 (S218/S222)/ MEK2 (S222/S226) at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MEK1/MEK2

MEK1 and MEK2, also known as MAP2K1 and MAP2K2, are activated in response to mitogenic stimuli. Activation occurs through dual phosphorylation at S218/S222 of MEK1 and S222/S226 of MEK2 by several upstream MAP3Ks, including the Raf kinases. Both MEK1 and MEK2 are dual-specificity protein kinases, phosphorylating and activating ERK1 and ERK2 at Thr and Tyr positions.

Long Name

Mitogen-activated Protein Kinase Kinase 1

Alternate Names

CFC3; CFC4; dual specificity mitogen-activated protein kinase kinase 1; dual specificity mitogen-activated protein kinase kinase 2; ERK activator kinase 1; ERK activator kinase 2; MAP kinase kinase 2; MAPK/ERK kinase 1; MAPK/ERK kinase 2; MAPKK 1; MAPKK1; MAPKK2; MEK 1; MEK1; MEK1/MEK2; MEK2; MEL; mitogen-activated protein kinase kinase 2, p45; MKK1; MKK2; PRKMK1; PRKMK2; protein kinase, mitogen-activated, kinase 1 (MAP kinase kinase 1)

Product Datasheets

Product Datasheet

COA

SDS

Product Specific Notices

* Contains <0.1% Sodium Azide, which is not hazardous at this concentration according to GHS classifications. Refer to SDS for additional information and handling instructions.