Human/Mouse/Rat PKC alpha Antibody

Catalog #: AF5340
3 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF5340
AF5340-SP

Detection of Human/Mouse/Rat PKC alpha by Western Blot.

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All PKC alpha Antibodies

Product Details

Citations (3)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse/Rat PKC alpha Antibody Summary

Species Reactivity

Human, Mouse, Rat

Specificity

Detects endogenous human, mouse, and rat PKC alpha in Western blots.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human PKC alpha
Lys604-Val672
Accession # P17252

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Immunohistochemistry

5-15 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human/Mouse/Rat PKC alpha by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, Saos-2 human osteosarcoma cell line, Balb/3T3 mouse embryonic fibroblast cell line, and NRK rat normal kidney cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat PKCa Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5340) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for PKCa at approximately 90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry

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PKC alpha in Human Breast Cancer Tissue. PKCa was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Goat Anti-Human/Mouse/Rat PKCa Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5340) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cancer cell cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PKC alpha

PKC alpha (Protein kinase C alpha) is an 80 kDa member of the PKC subfamily, AGC Ser/Thr protein kinase family of enzymes. It is widely expressed, and serves multiple cell-specific functions. PKC alpha is activated by increased diacylglycerol and intracellular calcium, and translocates to several sites such as the Golgi, nucleus and plasma membrane. Human PKC alpha is 672 amino acids (aa) in length. It contains two zinc finger regions (aa 36‑151), a protein kinase domain (aa 339‑557), and an AGC kinase region (aa 598‑668). Phosphorylations on Thr497, Thr638 and Ser657 are necessary for kinase activity. Over aa 604‑672, human PKC alpha shows 100% aa identity to mouse PKC alpha.

Long Name

Protein Kinase C alpha

Entrez Gene IDs

5578 (Human); 18750 (Mouse); 24680 (Rat); 447031 (Xenopus)

Alternate Names

AAG6; aging-associated gene 6; EC 2.7.11; EC 2.7.11.13; MGC129901; PKC alpha; PKCA; PKC-A; PKC-alpha; PKCAMGC129900; PRKACA; PRKCA; protein kinase C alpha type; protein kinase C, alpha

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human/Mouse/Rat PKC alpha Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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Small-Molecule-Directed Endogenous Regeneration of Visual Function in a Mammalian Retinal Degeneration Model
Authors: Mokady, D;Charish, J;Barretto-Burns, P;Grisé, KN;Coles, BLK;Raab, S;Ortin-Martinez, A;Müller, A;Fasching, B;Jain, P;Drukker, M;van der Kooy, D;Steger, M;
International journal of molecular sciences
Species: Murine polyomavirus strain A3
Sample Types: Whole Tissue
Applications: Immunohistochemistry
Electroacupuncture Inhibits the Activity of Astrocytes in Spinal Cord in Rats with Visceral Hypersensitivity by Inhibiting P2Y1 Receptor-Mediated MAPK/ERK Signaling Pathway
Authors: J Zhao, H Li, C Shi, T Yang, B Xu
Evid Based Complement Alternat Med, 2020-02-25;2020(0):4956179.
Species: Rat
Sample Types: Tissue Homogenates
Applications: Western Blot
Spinal PKC? inhibition and gene-silencing for pain relief: AMPAR trafficking at the synapses between primary afferents and sensory interneurons
Authors: O Kopach, V Krotov, A Shysh, A Sotnic, V Viatchenko, V Dosenko, N Voitenko
Sci Rep, 2018-07-06;8(1):10285.
Species: Rat
Sample Types: Tissue Homogenates
Applications: Western Blot