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Human/Mouse/Rat RIPK1/RIP1 Antibody

Catalog #: MAB3585
13 Citations 1 Review Datasheet / COA / SDS
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MAB3585
MAB3585-SP
Detection of Human/Mouse/Rat RIPK1/RIP1 by Western Blot.
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Product Details
Citations (13)
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Supplemental Products
Reviews (1)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse/Rat RIPK1/RIP1 Antibody Summary

Species Reactivity
Human, Mouse, Rat
Specificity
Detects human, mouse and rat RIPK1/RIP1 in Western blots.
Source
Monoclonal Mouse IgG1 Clone # 334640
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human RIPK1/RIP1
Met1-Asn671
Accession # Q13546
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.5 µg/mL
See below
Simple Western
10 µg/mL
See below
Immunocytochemistry
8-25 µg/mL
See below
Knockout Validated
RIPK1/RIP1 is specifically detected in MCF‑7 human breast cancer cell line parental cell line but is not detectable in RIPK1/RIP1 knockout MCF‑7 cell line.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human/Mouse/Rat RIPK1/RIP1 antibody by Western Blot. View Larger

Detection of Human/Mouse/Rat RIPK1/RIP1 by Western Blot. Western blot shows lysates of Raji human Burkitt's lymphoma cell line, Jurkat human acute T cell leukemia cell line, DA3 mouse myeloma cell line, and L6 rat myoblast cell line. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human/Mouse/Rat RIPK1/RIP1 Monoclonal Antibody (Catalog # MAB3585) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for RIPK1/RIP1 at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Immunocytochemistry RIPK1/RIP1 antibody in MCF-7 Human Cell Line by Immunocytochemistry (ICC). View Larger

RIPK1/RIP1 in MCF‑7 Human Cell Line. RIPK1/RIP1 was detected in immersion fixed MCF-7 human breast cancer cell line using Mouse Anti-Human/Mouse/Rat RIPK1/RIP1 Monoclonal Antibody (Catalog # MAB3585) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Simple Western Detection of Human RIPK1/RIP1 antibody by Simple Western<SUP>TM</SUP>. View Larger

Detection of Human RIPK1/RIP1 by Simple WesternTM. Simple Western lane view shows lysates of Jurkat human acute T cell leukemia cell line and MCF‑7 human breast cancer cell line, loaded at 0.2 mg/mL. A specific band was detected for RIPK1/RIP1 at approximately 78 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human/Mouse/Rat RIPK1/RIP1 Monoclonal Antibody (Catalog # MAB3585). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody.

Knockout Validated Western Blot Shows Human RIPK1/RIP1 Antibody Specificity by Using Knockout Cell Line. View Larger

Western Blot Shows Human RIPK1/RIP1 Specificity by Using Knockout Cell Line. Western blot shows lysates of MCF-7 human breast cancer parental cell line and RIPK1/RIP1 knockout MCF-7 cell line (KO). PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human/Mouse/Rat RIPK1/RIP1 Monoclonal Antibody (Catalog # MAB3585) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for RIPK1/RIP1 at approximately 75 kDa (as indicated) in the parental MCF-7 cell line, but is not detectable in knockout MCF-7 cell line. GAPDH (Catalog # MAB5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: RIPK1/RIP1

Receptor-Interacting Protein 1 (RIP1, also known as RIPK1) is a 671 amino acid (aa) 75 kDa protein that contains an N-terminal protein kinase domain, a C-terminal death domain, and a unique internal region called the intermediate domain. RIP1 is a serine/threonine protein kinase and is constitutively expressed in many tissues. RIP1 interacts with the cytoplasmic death domain of FAS and TNF receptors and is an important element in the signal transduction machinery that mediates apoptosis. RIP1 has been shown to interact with a number of proteins including TRADD, TRAF1, TRAF2, and TRAF3, to form larger signaling complexes. These complexes, in turn, activate specific signaling cascades, such as NF kappa B. RIP1 also interacts through the C-terminal RIP homotypic interaction motif (RHIM) of TRIF in TLR3 dependent activation of NF kappa B.

Long Name
Receptor (TNFRSF)-Interacting Serine-Threonine Kinase 1
Entrez Gene IDs
8737 (Human); 19766 (Mouse)
Alternate Names
Cell death protein RIP; EC 2.7.11.1; receptor (TNFRSF)-interacting serine-threonine kinase 1; receptor interacting protein; Receptor-interacting protein 1; receptor-interacting serine/threonine-protein kinase 1; RIP; Rip1 kinase; RIP1; RIP-1; RIPFLJ39204; RIPK1; Serine/threonine-protein kinase RIP

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Citations for Human/Mouse/Rat RIPK1/RIP1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

13 Citations: Showing 1 - 10
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  1. Anagliptin prevents lipopolysaccharide (LPS)- induced inflammation and activation of macrophages
    Authors: F Yu, W Tian, J Dong
    International immunopharmacology, 2022-01-16;104(0):108514.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. A Novel Method of Mouse RPE Explant Culture and Effective Introduction of Transgenes Using Adenoviral Transduction for In Vitro Studies in AMD
    Authors: P Shang, NA Stepicheva, H Liu, O Chowdhury, J Franks, M Sun, S Hose, S Ghosh, M Yazdankhah, A Strizhakov, DB Stolz, JS Zigler, D Sinha
    International Journal of Molecular Sciences, 2021-11-05;22(21):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  3. ABT?737, a Bcl?2 family inhibitor, has a synergistic effect with apoptosis by inducing urothelial carcinoma cell necroptosis
    Authors: R Cheng, X Liu, Z Wang, K Tang
    Molecular Medicine Reports, 2021-03-31;23(6):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  4. Inhibition of MLKL Attenuates Necroptotic Cell Death in a Murine Cell Model of Ischaemia Injury
    Authors: R Baidya, J Gautheron, DHG Crawford, H Wang, KR Bridle
    Journal of Clinical Medicine, 2021-01-08;10(2):.
    Species: Mouse
    Sample Types: Cell Lysates, Whole Cells
    Applications: Flow Cytometry, Western Blot
  5. Interaction between the BAG1S isoform and HSP70 mediates the stability of anti-apoptotic proteins and the survival of osteosarcoma cells expressing oncogenic MYC
    Authors: VJ Gennaro, H Wedegaertn, SB McMahon
    BMC Cancer, 2019-03-22;19(1):258.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  6. Leishmania braziliensis Subverts Necroptosis by Modulating RIPK3 Expression
    Authors: NF Luz, R Khouri, J Van Weyenb, DL Zanette, PP Fiuza, A Noronha, A Barral, VS Boaventura, DB Prates, FK Chan, BB Andrade, VM Borges
    Front Microbiol, 2018-09-28;9(0):2283.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  7. RIP3 dependent NLRP3 inflammasome activation is implicated in acute lung injury in mice
    Authors: J Chen, S Wang, R Fu, M Zhou, T Zhang, W Pan, N Yang, Y Huang
    J Transl Med, 2018-08-20;16(1):233.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  8. Necrostatin-1 protects C2C12 myotubes from CoCl2-induced hypoxia
    Authors: R Chen, J Xu, Y She, T Jiang, S Zhou, H Shi, C Li
    Int. J. Mol. Med., 2018-02-06;41(5):2565-2572.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  9. MPP+ induces necrostatin-1- and ferrostatin-1-sensitive necrotic death of neuronal SH-SY5Y cells
    Authors: K Ito, Y Eguchi, Y Imagawa, S Akai, H Mochizuki, Y Tsujimoto
    Cell Death Discov, 2017-02-27;3(0):17013.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  10. Hypoxia potentiates the cytotoxic effect of piperlongumine in pheochromocytoma models
    Oncotarget, 2016-06-28;7(26):40531-40545.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  11. ESCRT-0 dysfunction compromises autophagic degradation of protein aggregates and facilitates ER stress-mediated neurodegeneration via apoptotic and necroptotic pathways
    Authors: R Oshima, T Hasegawa, K Tamai, N Sugeno, S Yoshida, J Kobayashi, A Kikuchi, T Baba, A Futatsugi, I Sato, K Satoh, A Takeda, M Aoki, N Tanaka
    Sci Rep, 2016-04-26;6(0):24997.
    Species: Rat
    Sample Types: Cell Lysates
    Applications: Western Blot
  12. Open reading frame 3 of genotype 1 hepatitis E virus inhibits nuclear factor-kappaappa B signaling induced by tumor necrosis factor-alpha in human A549 lung epithelial cells.
    Authors: Xu J, Wu F, Tian D, Wang J, Zheng Z, Xia N
    PLoS ONE, 2014-06-24;9(6):e100787.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  13. TWEAK Induces Apoptosis through a Death-signaling Complex Comprising Receptor-interacting Protein 1 (RIP1), Fas-associated Death Domain (FADD), and Caspase-8.
    Authors: Ikner A, Ashkenazi A
    J. Biol. Chem., 2011-04-27;286(24):21546-54.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot

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Human/Mouse/Rat RIPK1/RIP1 Antibody
By Anonymous on 05/04/2020
Application: WB Sample Tested: AML12 (alpha mouse liver 12) cell Species: Mouse

Aml12 cell line. Cells treated with Nec-1 (RIPK1 inhibitor) for 24 hours.
Primary antibody: 1:1000
Secondary antibody: 1: 200000

Western Blot Human/Mouse/Rat RIPK1/RIP1 Antibody MAB3585