Human/Mouse/Rat RSK1 Antibody

Catalog #: AF992
1 Citation Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF992
AF992-SP

Detection of Human/Mouse/Rat RSK1 by Western Blot.

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Human/Mouse/Rat RSK1 Antibody Summary

Species Reactivity

Human, Mouse, Rat

Specificity

Detects human, rat and mouse RSK1. This antibody does not detect recombinant human RSK2.

Source

Polyclonal Rabbit IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human RSK1
Accession # Q15418

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Purity

Immunogen affinity purified

Applications

Recommended Concentration

Sample

Western Blot

0.2 µg/mL

See below

Immunohistochemistry

5-15 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human/Mouse/Rat RSK1 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, HepG2 human hepatocellular carcinoma cell line, TS1 mouse helper T cell line, and PC-12 rat adrenal pheochromocytoma cell line. PVDF membrane was probed with 0.2 µg/mL of Human/Mouse/Rat RSK1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF992) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for RSK1 at approximately 90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry

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RSK1 in Human Ovarian Cancer Tissue. RSK1 was detected in immersion fixed paraffin-embedded sections of human ovarian cancer tissue using Human/Mouse/Rat RSK1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF992) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rabbit HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS005) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: RSK1

The 90 kDa ribosomal protein S6 kinases (RSK1-4) are a family of broadly expressed Ser/Thr kinases activated in response to mitogenic stimuli, including growth factors and tumor-promoting phorbol esters. RSKs are directly phosphorylated by ERK1 and ERK2, leading to RSK autophosphorylation and activation. Active RSKs appear to play a major role in transcriptional regulation by translocating to the nucleus and phosphorylating c-Fos and CREB.

Long Name

Ribosomal Protein S6 Kinase I

Entrez Gene IDs

6195 (Human); 20111 (Mouse); 81771 (Rat)

Alternate Names

90kD, polypeptide 1); EC 2.7.11; EC 2.7.11.1,90 kDa ribosomal protein S6 kinase 1; HU-1; MAP kinase-activated protein kinase 1a; MAPK-activated protein kinase 1a; MAPKAP kinase 1a; MAPKAPK-1a; MAPKAPL1A; p90RSK1; p90S6K; ribosomal protein S6 kinase alpha 1; ribosomal protein S6 kinase alpha-1; ribosomal protein S6 kinase, 90kD, polypeptide 1; ribosomal protein S6 kinase, 90kDa, polypeptide 1; Ribosomal S6 kinase 1; RPS6KA1; RSK; RSK1; RSK-1; RSK1p90-RSK 1; S6K-alpha 1; S6K-alpha-1

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Related Research Areas

Citation for Human/Mouse/Rat RSK1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1