Human/Mouse/Rat SMC1 Antibody

Catalog #: MAB3294 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB3294
MAB3294-SP

Detection of Human/Mouse/Rat SMC1 by Western Blot.

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Human/Mouse/Rat SMC1 Antibody Summary

Species Reactivity

Human, Mouse, Rat

Specificity

Detects human, mouse and rat SMC1 in Western blots.

Source

Monoclonal Mouse IgG_2B Clone # 317705

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human SMC1
Met837-Gln1233
Accession # Q14683

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Applications

Recommended Concentration

Sample

Western Blot

0.25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human/Mouse/Rat SMC1 by Western Blot. Western blot shows lysates of U2OS human osteosarcoma cell line, Balb/3T3 mouse embryonic fibroblast cell line, and NRK rat normal kidney cell line. PVDF membrane was probed with 0.25 µg/mL of Human/Mouse/Rat SMC1 Monoclonal Antibody (Catalog # MAB3294) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for SMC1 at approximately 145 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SMC1

Structural Maintenance of Chromosome 1 (SMC1) is an ATPase which, with SMC3, forms a heterodimeric complex. These dimers form a V-shaped molecule with two long coiled-coil arms, each having an ATP-binding domain at its distal end. The SMC1/SMC3 heterodimer plays an important role in sister chromatid cohesion during metaphase, associates with the nuclear matrix and centrosome during interphase and localizes to kinetochores, spindles and spindle poles during mitosis. Smc1 is also a component of the DNA damage response and participates in the cellular response to DNA damage through its phosphorylation on Ser 957 and 966.

Long Name

Structural Maintenance of Chromosomes 1

Entrez Gene IDs

8243 (Human); 63996 (Rat)

Alternate Names

CDLS2; DXS423ESMC1 structural maintenance of chromosomes 1-like 1 (yeast); KIAA0178DKFZp686L19178; SB1.8; SB1.8MGC138332; segregation of mitotic chromosomes 1; SMC protein 1A; SMC1 (structural maintenance of chromosomes 1, yeast)-like 1; SMC1 structural maintenance of chromosomes 1-like 1; SMC1; SMC1A; SMC-1A; SMC-1-alpha; SMC1L1; SMC1SMC1alpha; Smcb; structural maintenance of chromosomes 1A; structural maintenance of chromosomes protein 1A